Search Results - (Author, Cooperation:L. J. Sweetlove)
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1Latest Papers from Table of Contents or Articles in PressM. Schwarzlander ; S. Wagner ; Y. G. Ermakova ; V. V. Belousov ; R. Radi ; J. S. Beckman ; G. R. Buettner ; N. Demaurex ; M. R. Duchen ; H. J. Forman ; M. D. Fricker ; D. Gems ; A. P. Halestrap ; B. Halliwell ; U. Jakob ; I. G. Johnston ; N. S. Jones ; D. C. Logan ; B. Morgan ; F. L. Muller ; D. G. Nicholls ; S. J. Remington ; P. T. Schumacker ; C. C. Winterbourn ; L. J. Sweetlove ; A. J. Meyer ; T. P. Dick ; M. P. Murphy
Nature Publishing Group (NPG)
Published 2014Staff ViewPublication Date: 2014-10-25Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Animals ; Caenorhabditis elegans/*metabolism ; *Longevity ; Male ; Mitochondria/*metabolism ; Superoxides/*metabolismPublished by: -
2Articles: DFG German National LicensesStaff View
ISSN: 1365-3040Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: BiologyNotes: The aim of this work was to investigate the effect of environmental conditions on the control of source to sink carbon flux in potato during tuberization. Top down metabolic control analysis was used to quantitatively determine the extent to which source and sink reactions control the flux from CO2 to the tuber. Under all conditions investigated (beginning of the dark period, low nitrogen availability, variation in temperature) source metabolism accounts for at least 80% of the control. In plants grown with limiting nitrogen, or when measurements were made at low temperature the extent to which source metabolism controls flux increased to 90%.Type of Medium: Electronic ResourceURL: -
3Articles: DFG German National LicensesSweetlove, L. J. ; Dunford, R. ; Ratcliffe, R. G. ; Kruger, N. J.
Oxford, UK : Blackwell Science Ltd
Published 2000Staff ViewISSN: 1365-3040Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: BiologyNotes: The aim of this work was to investigate the effect of decreased activity of lactate dehydrogenase (EC 1.1.1.27; LDH) on lactate metabolism in potato tubers. By expressing a cDNA-encoding potato tuber LDH in the antisense orientation, we generated transgenic potato plants with a preferential decrease in two of the five isozymes of LDH. Surprisingly, transgenic tubers grown under normoxic conditions did not contain less lactate, but rather instead contained approximately two-fold more lactate than control tubers. This result is explicable if the decreased isozymes of LDH are responsible for the oxidation of lactate to pyruvate in vivo. This was confirmed by measurements of the rate of metabolism of lactate supplied to tuber discs: the rate in transgenic tubers was approximately half that of control tubers. The decrease in LDH activity had no measurable effect on the accumulation of lactate in cold-stored tubers under anoxia, nor during the subsequent utilization of this lactate upon return to normoxia. In both control and transgenic tubers, the accumulation of lactate during anoxia was not accompanied by an induction of LDH activity or a change in isozyme distribution. In contrast, the metabolism of lactate after a period of anoxia was accompanied by a two-fold increase in LDH activity and the induction of two isozymes that were distinct from those which had been decreased in the transgenic plants.Type of Medium: Electronic ResourceURL: