Search Results - (Author, Cooperation:L. Fontana)

2012-03-03

American Association for the Advancement of Science (AAAS)

0036-8075

1095-9203

Biology

Chemistry and Pharmacology

Computer Science

Medicine

Natural Sciences in General

Physics

Base Sequence ; DNA, Chloroplast/genetics ; DNA, Mitochondrial/genetics ; *Ecosystem ; Europe ; *Fossils ; Geologic Sediments ; Haplotypes ; *Ice Cover ; Molecular Sequence Data ; Mutation ; Norway ; *Picea/genetics ; *Pinus/genetics ; Scandinavian and Nordic Countries ; Time

2014-07-25

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Age of Onset ; Aging/*drug effects/genetics/*physiology ; Animals ; Biomarkers ; Biomedical Research/economics/*trends ; Caloric Restriction ; Clinical Trials as Topic ; Diet ; Exercise ; Geriatrics/economics/*trends ; Health ; Humans ; Longevity/*drug effects/genetics/physiology ; Metformin/pharmacology ; Models, Animal ; Precision Medicine/trends ; Preventive Medicine/economics/*trends ; Rejuvenation/*physiology ; Sirolimus/pharmacology

2011-05-21

American Association for the Advancement of Science (AAAS)

0036-8075

1095-9203

Biology

Chemistry and Pharmacology

Computer Science

Medicine

Natural Sciences in General

Physics

*Adaptation, Physiological ; Amino Acids/biosynthesis ; Animals ; Bacteria/classification/genetics/metabolism ; Biological Evolution ; Biostatistics ; Caloric Restriction ; *Diet ; Enzymes/genetics/metabolism ; Feces/*microbiology ; Gastrointestinal Tract/*microbiology/physiology ; Genes, Bacterial ; Genes, rRNA ; Humans ; Least-Squares Analysis ; Mammals/*microbiology/physiology ; Metagenome/*physiology ; Monte Carlo Method ; *Phylogeny ; Proteins/metabolism ; RNA, Ribosomal, 16S/genetics ; Sequence Analysis, DNA

2018-12-15

The American Association for Cancer Research (AACR)

1078-0432

1557-3265

Medicine

1089-7690

AIP Digital Archive

Physics

Chemistry and Pharmacology

Vibrational Raman, infrared absorption, and infrared circular dichroism (VCD) spectra were measured for methylthiirane. Ab inito vibrational frequencies were calculated with a 6-31G basis, and vibrational assignments were suggested by comparing the experimental and theoretical data. These assignments were used for the interpretation of the experimental VCD spectrum of (R)-methylthiirane.

Electronic Resource

1365-3083

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

The complement system was examined in a group of eight patients (six with lymphoadenopathy syndrome (LAS); two with acquired immunodeficiency syndrome (AIDS)-related complex (ARC)), who were found to be human immunodeficiency virus (HIV)-positive, for the presence of specific HIV-anti-HIV complexes, A significant impartment of the classical and/or alternative pathway was found associated with the presence of cleavage fragments of C3 and/or B and a significant reduction in the complement factors studied. Ultracentrifugation fractions of serum samples obtained from one of the patients were assessed for the detection of specific HIV-anti-HIV (GP41-anti-GP41) complexes and were incubated with normal human serum to determine their complement activation capacity. A clear complement activation was found with the fraction in which a clear peak of HIV-anti-HIV (GP41-anti-GP41) immune complexes was present. The results demonstrate that specific immune complexes and complement activation are sometimes concomitantly present in patients with AIDS-related disease and that specific immune complexes may be one of the causal factors of the pathogenesis of complement activation in these patients. Possible consequences for the severe immune regulation with relevance to the dramatic failure in treating the virus effectively are discussed.

Electronic Resource

1398-9995

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

We recently described a protective effect of the low molecular weight protein tyrosine phosphatase (LMPTP) BC genotype, associated with the highest total enzymatic activity, against high serum IgE levels both in the English and the Italian populations. Here we test the hypothesis of a role of LMPTP in the negative modulation of IL-4 signal transduction checking for genetic interaction between interleukin-4 receptor alpha chain (IL-4RA) genetic polymorphisms and LMPTP polymorphism in the predisposition to high total IgE levels in the English population. We find a significant interaction between LMPTP polymorphism and the intracellular Gln/Arg polymorphism in position 551 of IL-4RA. Our data support the hypothesis of a direct or indirect biochemical interaction between LMPTP and IL-4RA resulting in different modulation of IL-4 signal transduction among joint genotypes.

Electronic Resource

1398-9995

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Atopic dermatitis (AD) is a chronic inflammatory skin disease frequently associated with asthma, rhinitis, and food allergy. Lymphocytes producing Th2-type cytokines (such as interleukin [IL]-3, IL-4, and IL-5) have been thought to have a key role in the pathogenesis of the disease. We have recently demonstrated that elevated serum levels of the soluble form of CD30 (sCD30), an activation marker of Th2-cell clones, correlates with disease activity in pediatric patients suffering from AD. Clinical trials have demonstrated that cyclosporin A (CyA) treatment resulted in significant improvement of clinical symptoms in patients affected with AD. In this study, we evaluated the role of CyA in modulating sCD30 release in a group of adult patients affected by severe AD treated with CyA at the dosage of 3.5 mg/kg body weight for 12 weeks. Our results demonstrated, in parallel with an improvement of clinical symptoms, a significant reduction of serum levels of both IL-4 and sCD30, thus suggesting that CyA can prevent the activation of Th2 cells observed in AD.

Electronic Resource

0020-711X

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Chemistry and Pharmacology

Electronic Resource

1398-9995

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Electronic Resource

1398-9995

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Electronic Resource

1420-9071

Springer Online Journal Archives 1860-2000

Biology

Medicine

Summary β-endorphin, met-enkephalin, leu-enkephalin and morphine significantly inhibit rosette formation between human T lymphocytes and sheep red blood cells. This effect is completely reversed by naloxone, a specific antagonist, while naloxone by itself does not influence rosette formation. A further link between the immune system and the neuroendocrine system is suggested.

Electronic Resource

1420-9071

Springer Online Journal Archives 1860-2000

Biology

Medicine

Summary The ultrastructural comparison between E and EA rosettes showed that, in the former, the rosetting lymphocytes are mostly round in shape and their interaction with sheep erythrocytes only consists of limited areas of membrane contact, in the latter, rosetting lymphocytes are mostly in the shape of uropods and surrounding ox red cells show pseudopods protruding towards the lymphocyte and coming into contact with it.

Electronic Resource

1420-9071

Springer Online Journal Archives 1860-2000

Biology

Medicine

Summary In the course of antibody-mediated lymphocyte cytotoxicity, ultrastructural studies show interaction between effector and target cells characterized by interdigitations. The significance of the observation is discussed.

Electronic Resource

1420-9071

Springer Online Journal Archives 1860-2000

Biology

Medicine

Summary The immuno-electronmicroscopic study of human EA rosettes, using ferritin-labelled anti-human IgG antiserum, showed clusters of ferritin granules at the points of contact between erythrocytes and lymphoid cells, indicating that the links between Fc fragment of IgG on the surface of erythrocytes and specific receptor on the surface of lymphoid cell correspond to the sites of morphological interaction between the 2 cell types.

Electronic Resource

1432-0584

Hairy Cell Leukemia ; NK activity ; Single cell cytotoxicity assay ; Membrane phenotype

Springer Online Journal Archives 1860-2000

Medicine

Summary Peripheral blood lymphocytes (PBL) of eleven patients with Hairy Cell Leukemia were studied for surface phenotype and for NK activity against the K 562 cell line (using both the standard 51Cr Release Assay and the Single Cell Cytotoxicity Assay on poly-L-lysine coated coverslips). A significant reduction in NK activity, target binding cells (TBC) and NK active cells (NKa) was detected. In some cases however, despite a very low percentage of NKa, residual NK activity was observed, suggesting an efficient recycling capacity.

Electronic Resource

1433-0369

Springer Online Journal Archives 1860-2000

Mathematics

Abstract. In this paper we propose a numerical method for the solution of the “Quasi-3D” hydrodynamic equations. This approach uses a combination of standard linear finite elements along the vertical direction and non-conforming Raviart-Thomas elements in the horizontal planes. We describe, also, a suitable approximation for the convective terms.

Electronic Resource

0263-6484

polyamines ; uptake ; efflux ; placenta ; JAR cells ; Life Sciences ; Molecular Cell Biology

Wiley InterScience Backfile Collection 1832-2000

Biology

Medicine

Uptake of polyamines has been investigated extensively in many cells, but not in placenta, where the polyamine- polyamine oxidase system is supposed to have an immunoregulatory function in pregnancy. Due to the importance of the transfer in this tissue, we have started this study. JAR human placental choriocarcinoma cells in monolayer at confluency were used as a model for measuring the key enzymes of polyamine synthesis and interconversion, rate of uptake and efflux, and the polyamine content. Polyamines were taken up by JAR cells and released by an independent mechanism. Ornithine decarboxylase and spermidine acetyltransferase activities and the rate of transport in and out of the cell were much higher than in other cells, such as L1210 cells. However the systems used for uptake and release appear in many respects to be similar to those observed in L1210 cells, but different from others. The uptake appears to be regulated by an inhibitory protein. Moreover, protein kinase C appears to be involved in the process. The efflux also is regulated as in L1210 cells, through control of H+ and Ca2+ concentration. In conclusion, this study shows that, in JAR cells, ornithine decarboxylase and spermidine acetyltransferase activities were much higher than in other cells, and so was the rate of transport in and out of the cells. As a result, a much higher polyamine content was observed.

2 Ill.

Electronic Resource