Search Results - (Author, Cooperation:L. Callegaro)

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  1. 1
    Staff View
    Publication Date:
    2013-07-13
    Publisher:
    American Association for the Advancement of Science (AAAS)
    Print ISSN:
    0036-8075
    Electronic ISSN:
    1095-9203
    Topics:
    Biology
    Chemistry and Pharmacology
    Computer Science
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Child ; Genetic Therapy/*methods ; Genetic Vectors ; *Hematopoietic Stem Cell Transplantation ; Hematopoietic Stem Cells/*metabolism ; Humans ; Lentivirus ; Male ; Transduction, Genetic ; Virus Integration ; Wiskott-Aldrich Syndrome/*therapy ; Wiskott-Aldrich Syndrome Protein/*genetics
    Published by:
    http://www.aaas.org/

    Latest Papers from Table of Contents or Articles in Press
  2. 2
    Staff View
    ISSN:
    0006-291X
    Keywords:
    [abr] BDNF; brain-derived neurotrophic factor ; [abr] CNS; central nervous system ; [abr] HPLC; high performance liquid chromatography ; [abr] IPTG; isopropyl-B-D-thiogalactopyranoside ; [abr] NGF; nerve growth factor ; [abr] PCR; polymerase chain reaction ; [abr] SDS-PAGE; SDS polyacrylamide gel electrophoresis
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Chemistry and Pharmacology
    Physics
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1016/S0006-291X(05)81584-0

    Articles: DFG German National Licenses
  3. 3
    Staff View
    ISSN:
    0003-2670
    Keywords:
    Bovine brain tissue ; DNA probes ; Gangliosides ; Pharmaceuticals ; Quality control
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Chemistry and Pharmacology
    Type of Medium:
    Electronic Resource
    URL:
    http://linkinghub.elsevier.com/retrieve/pii/0003-2670(91)87035-6

    Articles: DFG German National Licenses
  4. 4
    Staff View
    ISSN:
    0003-2670
    Keywords:
    Bovine brain tissue ; DNA probes ; Gangliosides ; Pharmaceuticals ; Quality control
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Chemistry and Pharmacology
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1016/0003-2670(91)87035-6

    Articles: DFG German National Licenses
  5. 5
    Staff View
    ISSN:
    0378-1119
    Keywords:
    Recombinant DNA ; dorsal root ganglion ; homodimer ; immunological cross-reactivity ; neurite outgrowth ; phage λ p"R and p"L promoters ; plasmid expression vector
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Type of Medium:
    Electronic Resource
    URL:
    http://linkinghub.elsevier.com/retrieve/pii/0378-1119(92)90657-B

    Articles: DFG German National Licenses
  6. 6
    Staff View
    ISSN:
    0301-6226
    Keywords:
    BSE ; Bovine spongiform encephalopathy ; Immunodiagnosis ; Prion protein
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1016/0301-6226(94)90241-0

    Articles: DFG German National Licenses
  7. 7
    Staff View
    ISSN:
    0008-6215
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Chemistry and Pharmacology
    Type of Medium:
    Electronic Resource
    URL:
    http://linkinghub.elsevier.com/retrieve/pii/0008-6215(92)84009-H

    Articles: DFG German National Licenses
  8. 8
    Staff View
    ISSN:
    0014-4827
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Medicine
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1016/0014-4827(91)90356-Y

    Articles: DFG German National Licenses
  9. 9
    Staff View
    ISSN:
    0731-7085
    Keywords:
    GM1 ; GM1 analysis ; cerebrospinal fluid ; competitive binding assay. ; ganglioside
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Chemistry and Pharmacology
    Medicine
    Type of Medium:
    Electronic Resource
    URL:
    http://linkinghub.elsevier.com/retrieve/pii/0731-7085(92)80024-H

    Articles: DFG German National Licenses
  10. 10
    Staff View
    ISSN:
    0006-291X
    Keywords:
    [abr] BDNF; brain-derived neurotrophic factor ; [abr] CNS; central nervous system ; [abr] HPLC; high performance liquid chromatography ; [abr] IPTG; isopropyl-B-D-thiogalactopyranoside ; [abr] NGF; nerve growth factor ; [abr] PCR; polymerase chain reaction ; [abr] SDS-PAGE; SDS polyacrylamide gel electrophoresis
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Chemistry and Pharmacology
    Physics
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1016/S0006-291X(05)81584-0

    Articles: DFG German National Licenses
  11. 11
    Staff View
    ISSN:
    0309-1651
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1016/0309-1651(90)90468-E

    Articles: DFG German National Licenses
  12. 12
    Staff View
    ISSN:
    1573-6903
    Keywords:
    CNTF ; neuroblastoma cells ; differentiation
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Medicine
    Notes:
    Abstract We have analyzed the response of the human neuroblastoma cell lines SK-N-SH (clone SY5Y) and SK-N-BE to the ciliary neurotrophic factor CNTF. In both cell lines CNTF induced the expression of the mRNA for two transcription factors, c-fos and NGF1A. The induction was rapid and transient reaching a maximum between 30 and 60 min after exposure to CNTF and subsequently declining. The level of induction of both c-fos and NGF1A mRNAs was much higher in SK-N-BE neuroblastoma cells compared to the SY5Y. Both cells express comparable levels of the transcript for the CNTF receptor-alpha. This mRNA was down regulated after 5 days of CNTF stimulation in both cell lines. CNTF also induced increased levels of the transcript for the growth cone associated protein GAP43 in SK-N-BE, but not in SY5Y cells. Induction followed a slower kinetic compared to that observed for c-fos and NGF1A. In fact, the GAP43 mRNA levels increased during 2 days of exposure to CNTF. Morphological analysis of CNTF treated cells showed that SK-N-BE undergo significant differentiation in response to CNTF (increased number of cells with neurites and increased neurite length) while SY5Y did not show appreciable morphological differentiation. These data shows that CNTF may elicit different response in neuroblastoma cell lines.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/BF01705535

    Articles: DFG German National Licenses
  13. 13
    Staff View
    ISSN:
    1590-3478
    Keywords:
    Nerve growth factor (NGF) ; cerebrospinal fluid (CSF) ; neurological diseases ; multiple sclerosis (MS) ; amyotrophic lateral sclerosis (ALS)
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Medicine
    Description / Table of Contents:
    Sommario È stato recentemente dimostrato che l'NGF non è solamente coinvolto nella sopravvivenza e lo sviluppo dei neuroni del sistema simpatico e in quelli sensitivi derivati dalla cresta neurale, ma anche in alcuni meccanismi del sistema immunitario. Per questo motivo abbiamo studiato il contenuto di NGF nel liquor di pazienti affetti da malattie quali la sclerosi multipla, la sclerosi laterale amiotrofica, la malattia di Alzheimer, le polineuropatie croniche recidivanti, la sindrome di Guillain-Barré e i tumori del sistema nervoso, nelle quali meccanismi neuroimmunologici paiono essere coinvolti, ed inoltre in un gruppo di soggetti neurologicamente sani presi come controlli. Abbiamo messo a punto una metodica ELISA per dosare la subunità beta dell'NGF, ottenendo buoni test di validazione e sensibilità fino a 28 pg βNGF per ml. Nessuno dei campioni conteneva livelli misurabili di NGF. Abbiamo quindi concentrato i campioni, ma soltanto un paziente ha presentato livelli significativi. Questo probabilmente mostra che l'NGF non è coinvolto nei meccanismi neuroimmunologici che potrebbero essere alla base di alcune malattie degenerative ed infiammatorie del sistema nervoso.
    Notes:
    Abstract It has been recently shown that NGF is not only involved in the survival and development of sympathetic and neural crest-derived sensory neurons, but also in some mechanisms of the immune system. For this reason, we studied the content of NGF in CSF samples from patients with diseases in which neuroimmonological mechanisms seem to be involved (multiple sclerosis, amyotrophic lateral sclerosis, Alzheimer disease, chronic relapsing polyradiculoneuritis, Guillain-Barré syndrome, and tumors of the nervous system), as well as from a number of normal control subjects. We setup an ELISA aimed at the beta subunit of NGF, obtaining good validation tests and a detection limit of 28 pg βNGF per ml. None of the samples was found to contain detectable levels of NGF and, when a concentration method for sample enrichment was used, only one patient was NGF-positive. This suggests that NGF is probably not involved in the neuroimmunological mechanisms underlying some inflammatory and degenerative diseases of the nervous system.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/BF02340121

    Articles: DFG German National Licenses
  14. 14
    Callegaro, L. ; Skaper, S. D. ; Vantini, G. ; Benvegnù, D. ; Di Martino, A. ; Schiavo, N. ; Triban, C. ; Minozzi, C. ; Leon, A.

    Chicester [u.a.] : Wiley-Blackwell
    Published 1990
    Staff View
    ISSN:
    0952-3499
    Keywords:
    Chemistry ; Biochemistry and Biotechnology
    Source:
    Wiley InterScience Backfile Collection 1832-2000
    Topics:
    Medicine
    Notes:
    A functional role for Nerve Growth Factor (NGF) in the peripheral nervous system is well-documented, but a similar case for NGF in the central nervous system remains to be established. One approach to answering this question would be the availability of high-affinity monospecific Fab fragments obtained against NGF. In the present studies we describe the preparation and characterization of such Fab fragments from anti-mouse NGF polyclonal antibodies. Following their purification by the use of a NGF Sepharose-coupled affinity column, the Fab fragments were examined for biological competence in several ways. In vitro, the anti-Fab fragments blcoked the neuronotrophic activity of NGF, as measured by the survival of chicken embryonic day 8 dorsal root ganglion neurons. In vivo, these Fab fragments, when administered systemically to neonatal rats, produced a decrease of noradrenaline levels in two sympathetically innervated organs, the heart and the spleen. These findings suggest that affinity purified Fab fragments of anti-NGF antibodies can be a useful tool for studying the physiological function of NGF in the nervous system.
    Additional Material:
    4 Ill.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1002/jmr.300030503

    Articles: DFG German National Licenses