Search Results - (Author, Cooperation:L. Bolund)

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  1. 1
    M. A. Groenen ; A. L. Archibald ; H. Uenishi ; C. K. Tuggle ; Y. Takeuchi ; M. F. Rothschild ; C. Rogel-Gaillard ; C. Park ; D. Milan ; H. J. Megens ; S. Li ; D. M. Larkin ; H. Kim ; L. A. Frantz ; M. Caccamo ; H. Ahn ; B. L. Aken ; A. Anselmo ; C. Anthon ; L. Auvil ; B. Badaoui ; C. W. Beattie ; C. Bendixen ; D. Berman ; F. Blecha ; J. Blomberg ; L. Bolund ; M. Bosse ; S. Botti ; Z. Bujie ; M. Bystrom ; B. Capitanu ; D. Carvalho-Silva ; P. Chardon ; C. Chen ; R. Cheng ; S. H. Choi ; W. Chow ; R. C. Clark ; C. Clee ; R. P. Crooijmans ; H. D. Dawson ; P. Dehais ; F. De Sapio ; B. Dibbits ; N. Drou ; Z. Q. Du ; K. Eversole ; J. Fadista ; S. Fairley ; T. Faraut ; G. J. Faulkner ; K. E. Fowler ; M. Fredholm ; E. Fritz ; J. G. Gilbert ; E. Giuffra ; J. Gorodkin ; D. K. Griffin ; J. L. Harrow ; A. Hayward ; K. Howe ; Z. L. Hu ; S. J. Humphray ; T. Hunt ; H. Hornshoj ; J. T. Jeon ; P. Jern ; M. Jones ; J. Jurka ; H. Kanamori ; R. Kapetanovic ; J. Kim ; J. H. Kim ; K. W. Kim ; T. H. Kim ; G. Larson ; K. Lee ; K. T. Lee ; R. Leggett ; H. A. Lewin ; Y. Li ; W. Liu ; J. E. Loveland ; Y. Lu ; J. K. Lunney ; J. Ma ; O. Madsen ; K. Mann ; L. Matthews ; S. McLaren ; T. Morozumi ; M. P. Murtaugh ; J. Narayan ; D. T. Nguyen ; P. Ni ; S. J. Oh ; S. Onteru ; F. Panitz ; E. W. Park ; H. S. Park ; G. Pascal ; Y. Paudel ; M. Perez-Enciso ; R. Ramirez-Gonzalez ; J. M. Reecy ; S. Rodriguez-Zas ; G. A. Rohrer ; L. Rund ; Y. Sang ; K. Schachtschneider ; J. G. Schraiber ; J. Schwartz ; L. Scobie ; C. Scott ; S. Searle ; B. Servin ; B. R. Southey ; G. Sperber ; P. Stadler ; J. V. Sweedler ; H. Tafer ; B. Thomsen ; R. Wali ; J. Wang ; S. White ; X. Xu ; M. Yerle ; G. Zhang ; J. Zhang ; S. Zhao ; J. Rogers ; C. Churcher ; L. B. Schook
    Nature Publishing Group (NPG)
    Published 2012
    Staff View
    Publication Date:
    2012-11-16
    Publisher:
    Nature Publishing Group (NPG)
    Print ISSN:
    0028-0836
    Electronic ISSN:
    1476-4687
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Animals ; Demography ; Genome/*genetics ; Models, Animal ; Molecular Sequence Data ; *Phylogeny ; Population Dynamics ; Sus scrofa/*classification/*genetics
    Published by:
    http://www.nature.com/

    Latest Papers from Table of Contents or Articles in Press
  2. 2
    Toldbod, H. E. ; Agger, R. ; Bolund, L. ; Hokland, M.

    Oxford, UK : Blackwell Science Ltd
    Published 2003
    Staff View
    ISSN:
    1365-3083
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Medicine
    Notes:
    Typically autologous dendritic cells (DCs) intended for vaccination are generated from bone marrow derived stem cells or blood monocytes, loaded with antigen and introduced into the organism. However, addition of serum to DC culture medium is often necessary. Thus, serum proteins will be taken up and presented by the DCs together with other antigens. If heterologous serum is used, some of the serum proteins might be antigenic and thus induce a strong immune response when introduced in the recipient. We used the murine model of malignant melanoma, B16, to investigate the consequences of addition of fetal calf serum (FCS) to the medium for culturing murine DCs. The results showed that vaccination of mice with DCs cultured in vitro in the presence of FCS but in the absence of extraneous tumour antigens, protected the mice from challenge with B16 tumour cells similarly cultured in FCS. This protection could not be elicited by vaccination with FCS alone. Interestingly, the protective effect of DC vaccination was abolished when the challenging B16 tumour cells were free of serum proteins. Thus, these results show that DCs grown in the presence of FCS are able to induce immunity, which may be mistaken to be tumour immunity.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1046/j.1365-3083.2003.01267.x

    Articles: DFG German National Licenses
  3. 3
    Jensen, U. B. ; Petersen, M. S. ; Lund, T. B. ; Jensen, T. G. ; Bolund, L.

    Copenhagen : Munksgaard International Publishers
    Published 2000
    Staff View
    ISSN:
    1600-0625
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Medicine
    Notes:
    Abstract: We have analysed the consequences of liposome mediated gene transfer into human primary epidermal keratinocytes and compared non-Epstein–Barr Virus (EBV) and EBV based expression vectors that carry the genes encoding human Growth Hormone (hGH) or Enhanced Green Fluorescent Protein (EGFP). Different kinetics between the non-EBV and EBV based vectors were revealed upon subcultivation of hGH transfected keratinocytes. The keratinocytes transfected with non-EBV based vector showed a rapid reduction in hGH production. Although the EBV based vector resulted in more stable expression, this was also reduced over time. Chromatin inactivation by deacetylation was investigated by treatment with sodium butyrate and found not to be the reason for the decreasing expression. Keratinocytes divided into subpopulations enriched for either stem cells or transit amplifying cells, based on β1-integrin expression and function, do not differ significantly with respect to susceptibility to productive transfection. However, when the keratinocytes were transfected with the EGFP gene and sorted live by FACS into EGFP negative and positive populations, only the negative cells were capable of forming significant numbers of colonies. This is consistent with the observation that the ability to incorporate BrdU was dramatically reduced in the EGFP expressing population within 24–48 h post transfection indicating an almost complete cell cycle arrest. p53 levels were unaffected by the procedures, and the keratinocyte cell line HaCat, mutated in both p53 alleles, also shows a marked reduction in clonogenic potency upon transfection. There was a slight increase of TUNEL positive apoptotic nuclei in the positive population at early time points. However, the apoptotic index was still very low. When we measured the frequency of involucrin expressing cells, we found an increase in the productively transfected population over time indicating an initiation of terminal differentiation. In contrast to the transfected cultures, keratinocytes that were transduced using a retroviral vector showed no decrease in colony forming efficiency. In conclusion we find that transgene expressing cells from transfected cultures of epidermal keratinocytes undergo cell cycle arrest and initiate terminal differentiation by mechanisms which are independent of p53 levels.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1034/j.1600-0625.2000.009004298.x

    Articles: DFG German National Licenses
  4. 4
    Staff View
    ISSN:
    1546-1718
    Source:
    Nature Archives 1869 - 2009
    Topics:
    Biology
    Medicine
    Notes:
    [Auszug] Comparative genomic hybridization using arrays of genomic cosmid, P1 and BAC clones (array CGH) provides quantitative copy number data over a wide dynamic range with resolution determined solely by the size and genomic spacing of the arrayed clones. We have demonstrated previously that array CGH ...
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1038/14259

    Articles: DFG German National Licenses
  5. 5
    Staff View
    ISSN:
    1546-1718
    Source:
    Nature Archives 1869 - 2009
    Topics:
    Biology
    Medicine
    Notes:
    [Auszug] We have mapped the autosomal sex reversal locus, SRA1, associated with campomelic dysplasia (CMPD1) to 17q24.3–q25.1 by three independent apparently balanced de novo reciprocal translocations. Chromosome painting indicates that the translocated segment of 17q involves about 15% of chromosome ...
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1038/ng0693-170

    Articles: DFG German National Licenses
  6. 6
    Ringertz, N.R. ; Bolund, L.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0005-2787
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Type of Medium:
    Electronic Resource
    URL:
    http://linkinghub.elsevier.com/retrieve/pii/0005-2787(69)90237-8

    Articles: DFG German National Licenses
  7. 7
    Staff View
    ISSN:
    0009-8981
    Keywords:
    Bio-dUTP ; Biotinylated DNA-probe ; Non-radioactive DNA-probe ; RFLP analysis
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Medicine
    Type of Medium:
    Electronic Resource
    URL:
    http://linkinghub.elsevier.com/retrieve/pii/0009-8981(87)90327-5

    Articles: DFG German National Licenses
  8. 8
    Staff View
    ISSN:
    0925-4439
    Keywords:
    COS-7 cell ; EBV-based expression vector ; G985 MCAD mutation ; Protein instability ; Subcellular location
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Physics
    Type of Medium:
    Electronic Resource
    URL:
    http://linkinghub.elsevier.com/retrieve/pii/0925-4439(92)90028-L

    Articles: DFG German National Licenses
  9. 9
    Staff View
    ISSN:
    0925-4439
    Keywords:
    Aggregation ; Chaperonin ; Folding ; GroE ; MCAD deficiency ; Oligomer assembly
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Physics
    Type of Medium:
    Electronic Resource
    URL:
    http://linkinghub.elsevier.com/retrieve/pii/0925-4439(93)90068-C

    Articles: DFG German National Licenses
  10. 10
    Staff View
    ISSN:
    0009-8981
    Keywords:
    (PCR) ; Allele specific oligonucleotide analysis ; Biotinylated oligonucleotides ; Enzymatic amplification ; Polymerase chain reaction ; alpha-1-Antitrypsin
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Medicine
    Type of Medium:
    Electronic Resource
    URL:
    http://linkinghub.elsevier.com/retrieve/pii/0009-8981(89)90074-0

    Articles: DFG German National Licenses
  11. 11
    Staff View
    ISSN:
    0009-8981
    Keywords:
    (MCAD) ; Dried blood spots ; G985 (PCR) ; G985 MCAD mutation ; Medium-chain acyl-CoA dehydrogenase deficiency ; polymerase chain reaction assay ; β-Oxidation defect
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Medicine
    Type of Medium:
    Electronic Resource
    URL:
    http://linkinghub.elsevier.com/retrieve/pii/0009-8981(91)90153-4

    Articles: DFG German National Licenses
  12. 12
    Ringertz, N.R. ; Bolund, L. ; Debault, L.E.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0014-4827
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Medicine
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1016/0014-4827(67)90157-7

    Articles: DFG German National Licenses
  13. 13
    Staff View
    ISSN:
    0014-4827
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Medicine
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1006/excr.1993.1326

    Articles: DFG German National Licenses
  14. 14
    Bolund, L.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0014-4827
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Medicine
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1016/0014-4827(70)90346-0

    Articles: DFG German National Licenses
  15. 15
    Staff View
    ISSN:
    0014-4827
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Medicine
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1016/0014-4827(91)90459-8

    Articles: DFG German National Licenses
  16. 16
    Liedeman, R. ; Bolund, L.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0014-4827
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Medicine
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1016/0014-4827(76)90426-2

    Articles: DFG German National Licenses
  17. 17
    Liedeman, R. ; Bolund, L.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0014-4827
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Medicine
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1016/0014-4827(76)90427-4

    Articles: DFG German National Licenses
  18. 18
    Darzynkiewicz, Z. ; Bolund, L. ; Ringertz, N.R.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0014-4827
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Medicine
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1016/0014-4827(69)90465-0

    Articles: DFG German National Licenses
  19. 19
    Jensen, P.K.A. ; Bolund, L.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0014-4827
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Medicine
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1016/0014-4827(88)90255-8

    Articles: DFG German National Licenses
  20. 20
    Appels, R. ; Bolund, L. ; Goto, S. ; Ringertz, N.R.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0014-4827
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Medicine
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1016/0014-4827(74)90229-8

    Articles: DFG German National Licenses