Search Results - (Author, Cooperation:K. Shirasu)

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  1. 1
    C. E. Conn ; R. Bythell-Douglas ; D. Neumann ; S. Yoshida ; B. Whittington ; J. H. Westwood ; K. Shirasu ; C. S. Bond ; K. A. Dyer ; D. C. Nelson
    American Association for the Advancement of Science (AAAS)
    Published 2015
    Staff View
    Publication Date:
    2015-08-01
    Publisher:
    American Association for the Advancement of Science (AAAS)
    Print ISSN:
    0036-8075
    Electronic ISSN:
    1095-9203
    Topics:
    Biology
    Chemistry and Pharmacology
    Computer Science
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Arabidopsis/*metabolism/*parasitology ; Arabidopsis Proteins/*classification/genetics/metabolism ; *Biological Evolution ; Gene Dosage ; Germination ; Heterocyclic Compounds, 1-Ring/*metabolism ; Host-Parasite Interactions ; Hydrolases/*classification/genetics/metabolism ; Lactones/*metabolism ; Orobanchaceae/*enzymology/genetics/growth & development ; Phylogeny ; Plant Growth Regulators/*metabolism ; Plant Roots/metabolism/parasitology ; Plants, Genetically Modified/genetics/metabolism
    Published by:
    Latest Papers from Table of Contents or Articles in Press
  2. 2
    Wakatake, T., Yoshida, S., Shirasu, K.
    The Company of Biologists
    Published 2018
    Staff View
    Publication Date:
    2018-08-02
    Publisher:
    The Company of Biologists
    Print ISSN:
    0950-1991
    Electronic ISSN:
    1477-9129
    Topics:
    Biology
    Keywords:
    Plant development
    Published by:
    Latest Papers from Table of Contents or Articles in Press
  3. 3
    Shirasu, K. ; Morel, P. ; Kado, C. I.

    Oxford, UK : Blackwell Publishing Ltd
    Published 1990
    Staff View
    ISSN:
    1365-2958
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Biology
    Medicine
    Notes:
    The virulence regulon of the Agrobacterium tumefaciens TiC58 plasmid is composed of six operons, virA, virB, virG, virC, virD and virE, which direct the transfer of T-DNA into plant cells. The 9.5kbp virB operon is the largest of these operons and its entire nucleotide sequence was determined and found to contain eleven open reading frames (ORFs). Gene fusions of each VirB ORF to T7φ10 were made and over-expressed in Escherichia coli to confirm that they encode proteins of predicted size. Hydrophobic analysis of these peptide sequences revealed nine proteins that contain hydrophobic spanning regions including signal-peptide-like sequences. These data suggest that the majority of VirB proteins may associate with bacterial cell membranes, while the two additional proteins possess a potential ATP-binding site. Strong homologies in amino acid sequences were observed between nopaline- and octopine-type plasmids. Specific differences in amino acid sequence encoded by VirB ORFs of nopaline and octopine Ti plasmid and a functional role of the gene products are discussed.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  4. 4
  5. 5
    Staff View
    ISSN:
    1432-119X
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Medicine
    Notes:
    Summary The distribution of estrogen target neurons which contain atrial natriuretic factor (ANF) in female rat hypothalamus was investigated by thaw-mount autoradiography combined with immunocytochemistry using tritium-labeled estradiol and antibodies against ANF. Colocalization of the two hormones was found in the arcuate nucleus, periventricular nucleus, lateral ventromedial nucleus, ventral premammillar nucleus and lateral basal hypothalamus. The percentage of ANF containing cells which concentrate estradiol varies among the different hypothalamic nuclei with the highest number of ANF-positive cells showing nuclear concentration of 3H-estradiol (80–90%) in the nucleus premammillaris ventralis, but less (5–15%) in the other nuclei. These data, together with topographical correspondence in extrahypothalamic brain regions between sites of action of estradiol and production of ANF, suggest extensive interrelationships and modulatory effects of estradiol on ANF production and secretion in the brain, similar to the atrium of the heart.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  6. 6
    Lahaye, T. ; Shirasu, K. ; Schulze-Lefert, P.
    Springer
    Published 1998
    Staff View
    ISSN:
    1617-4623
    Keywords:
    Key words Resistance reaction ; Powdery mildew ; Yeast artificial chromosome (YAC) ; Map-based cloning ; Cleaved amplified polymorphic sequence (CAPS)
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Notes:
    Abstract The barley Rar1 gene is an essential component of the race-specific, Mla-12-specified powdery mildew resistance reaction. As part of a map-based cloning strategy designed to isolate Rar1, five barley yeast artificial chromosomes (YACs) have been identified, ranging in size from 300 to 1100 kb. PCR-based YAC end-specific markers have been established and were employed to construct a local YAC contig. Four out of five YAC clones were found to be non-colinear with the source DNA. High-resolution genetic mapping of the YAC ends demonstrated that the set of five overlapping YAC clones encompasses the barley Rar1 gene.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses