Search Results - (Author, Cooperation:K. Shekhar)

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  1. 1
    Staff View
    Publication Date:
    2013-11-01
    Publisher:
    Nature Publishing Group (NPG)
    Print ISSN:
    0028-0836
    Electronic ISSN:
    1476-4687
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Animals ; Antibodies, Monoclonal/*therapeutic use ; Antibodies, Neutralizing/*therapeutic use ; DNA, Viral/blood ; HIV Antibodies/immunology ; HIV-1/*immunology ; Macaca mulatta ; Simian Acquired Immunodeficiency Syndrome/*therapy ; Simian Immunodeficiency Virus/*physiology ; T-Lymphocytes/immunology ; Viremia/therapy
    Published by:
    http://www.nature.com/

    Latest Papers from Table of Contents or Articles in Press
  2. 2
    Farrell, J. A., Wang, Y., Riesenfeld, S. J., Shekhar, K., Regev, A., Schier, A. F.
    American Association for the Advancement of Science (AAAS)
    Published 2018
    Staff View
    Publication Date:
    2018-06-01
    Publisher:
    American Association for the Advancement of Science (AAAS)
    Print ISSN:
    0036-8075
    Electronic ISSN:
    1095-9203
    Topics:
    Biology
    Chemistry and Pharmacology
    Geosciences
    Computer Science
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Development, Online Only
    Published by:
    http://www.aaas.org/

    Latest Papers from Table of Contents or Articles in Press
  3. 3
    Staff View
    Publication Date:
    2018-11-16
    Publisher:
    American Association for the Advancement of Science (AAAS)
    Print ISSN:
    0036-8075
    Electronic ISSN:
    1095-9203
    Topics:
    Biology
    Chemistry and Pharmacology
    Geosciences
    Computer Science
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Neuroscience, Online Only
    Published by:
    http://www.aaas.org/

    Latest Papers from Table of Contents or Articles in Press
  4. 4
    Staff View
    ISSN:
    0268-2575
    Keywords:
    immobilized enzymes ; site-specific modification of enzyme ; polymeric membranes ; kinetics ; Chemistry ; Biochemistry and Biotechnology
    Source:
    Wiley InterScience Backfile Collection 1832-2000
    Topics:
    Process Engineering, Biotechnology, Nutrition Technology
    Notes:
    A comparison of enzyme activities has been made between a site-specifically immobilized and a randomly immobilized bacterial alkaline phosphatase (BAP) on macroporous membranes. An octapeptide tag (FLAG™) was attached at the N-terminus of alkaline phosphatase by recombinant DNA techniques (gene fusion) to yield BAP that is modified in a site-directed fashion (SDBAP). The corresponding antibody (antiFLAG™) was immobilized on an aldehyde-modified polyethersulfone (MPS) membrane via protein A. Immobilization of SDBAP on this membrane result in a membrane-protein A-antiFLAG-SDBAP linkage. This site-specifically immobilized enzyme demonstrated a relative activity (RA), defined as the ratio of immobilized activity (Vmax) to the corresponding homogeneous enzymatic activity, of 85% as compared with the randomly immobilized BAP which had an RA of 0·8%. BAP, when chemically conjugated to the FLAG peptide and immobilized via antiFLAG and protein A on the MPS membrane, showed an RA of only 1·9%, demonstrating the effectiveness of site-directed immobilization. SDBAP was also immobilized on the MPS membrane in the absence of protein A. In this case, the RA dropped to 22%, further explaining the effectiveness of ordered immobilizations as compared with random immobilizations. The ratio of immobilized enzyme activity to the activity in the absence of added phosphate inhibitor for the immobilized BAP was three-fold higher than the corresponding homogeneous ratio, showing a reduction in product inhibition for the immobilized enzyme. © 1997 SCI.
    Additional Material:
    7 Ill.
    Type of Medium:
    Electronic Resource

    Articles: DFG German National Licenses