Search Results - (Author, Cooperation:K. Perry)

2015-02-14

American Association for the Advancement of Science (AAAS)

0036-8075

1095-9203

Biology

Chemistry and Pharmacology

Computer Science

Medicine

Natural Sciences in General

Physics

Amino Acid Sequence ; Catalytic Domain ; Conserved Sequence ; Crystallography, X-Ray ; Hepacivirus/enzymology/genetics/*physiology ; Molecular Sequence Data ; Protein Structure, Secondary ; RNA Replicase/*chemistry ; RNA, Viral/*biosynthesis ; Ribonucleotides/*chemistry ; Sofosbuvir ; Uridine Monophosphate/analogs & derivatives/chemistry ; Viral Nonstructural Proteins/*chemistry ; *Virus Replication

2012-03-01

American Association for the Advancement of Science (AAAS)

0036-8075

1095-9203

Biology

Chemistry and Pharmacology

Computer Science

Medicine

Natural Sciences in General

Physics

Amino Acid Sequence ; Bacterial Proteins/*chemistry/metabolism ; Binding Sites ; Botulinum Toxins, Type A/*chemistry/metabolism ; Crystallography, X-Ray ; Hydrogen-Ion Concentration ; Models, Molecular ; Molecular Sequence Data ; Multiprotein Complexes/chemistry/metabolism ; Mutagenesis ; Protein Binding ; Protein Conformation ; Protein Interaction Domains and Motifs ; Protein Structure, Secondary

2014-06-21

American Association for the Advancement of Science (AAAS)

0036-8075

1095-9203

Biology

Chemistry and Pharmacology

Computer Science

Medicine

Natural Sciences in General

Physics

Animals ; Botulinum Toxins, Type A/*chemistry/genetics ; Cadherins/*chemistry/genetics ; Crystallography, X-Ray ; Gene Knockdown Techniques ; HT29 Cells ; Hemagglutinins/*chemistry/genetics ; Humans ; Mice ; Protein Structure, Secondary ; Recombinant Proteins/chemistry

2014-09-05

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Arabidopsis/chemistry ; Bacterial Proteins/*chemistry/metabolism ; Crystallography, X-Ray ; Evolution, Molecular ; Glucose/metabolism ; Leptospira/*chemistry/genetics ; Models, Molecular ; Monosaccharide Transport Proteins/*chemistry/genetics/metabolism ; Movement ; Protein Conformation ; Protein Multimerization ; Structure-Activity Relationship ; Vibrio/*chemistry

2015-10-20

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Arabidopsis/chemistry ; Arabidopsis Proteins/chemistry/genetics/metabolism ; Biological Transport ; Crystallography, X-Ray ; Glucose Transport Proteins, Facilitative/*chemistry/genetics/metabolism ; HEK293 Cells ; Humans ; Models, Molecular ; Monosaccharide Transport Proteins/chemistry/genetics/metabolism ; Oryza/*chemistry/genetics ; Phloem ; Plant Proteins/*chemistry/metabolism ; *Protein Multimerization ; Protein Structure, Quaternary ; Protein Structure, Secondary ; Structure-Activity Relationship

2018-05-11

American Association for the Advancement of Science (AAAS)

0036-8075

1095-9203

Biology

Chemistry and Pharmacology

Geosciences

Computer Science

Medicine

Natural Sciences in General

Physics

Biochemistry, Microbiology

2018-06-29

American Society of Hematology (ASH)

0006-4971

1528-0020

Biology

Medicine

Immunobiology and Immunotherapy, Lymphoid Neoplasia

2018-06-30

The American Society for Microbiology (ASM)

0022-538X

1098-5514

Medicine

1365-3059

Blackwell Publishing Journal Backfiles 1879-2005

Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

A varied population of Cucumber Mosaic Virus (CMV) isolates is reported from peppers (Capsicum annuum) in Australia. Isolates representing both CMV subgroups (serogroups) I and II have been obtained from the same field at the same sampling time. The CMV isolates were typed into subgroup I (eight isolates) and subgroup II (two isolates) using both a nucleic acid hybridization assay and an immunological assay with monoclonal antibodies. The immunological assay described allows the typing of strains in crude sap extracts, obviating the need for purified virions. The spatial and temporal coincidence of both CMV subgroups presents a situation in which pseudorecombinants with reassorted genomic components might arise.

Electronic Resource

0034-5687

Blood ; Diffusing capacity, lung, hypoxia, exercise ; Equilibrium curve ; Exercise, hypoxia, pulmonary gas exchange (rat) ; Gas exchange, pulmonary, exercise, hypoxic rat ; Hypoxia, exercise, pilmonary gas exchange (rat) ; Mammals ; Rat ; Temperature coefficients

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Medicine

Electronic Resource

1399-3054

Blackwell Publishing Journal Backfiles 1879-2005

Biology

Transfer RNA metabolism in developmentally-abnormal ove strains of Physcomitrella patens (Hedw.) Br. Eur. which produce more than 100 times the wild-type level of cytokinin, was analysed. tRNA from ove and wild-type strains of P. patens was extracted and characterised and tRNA metabolism in these strains was compared. No differences large enough to account for the observed levels of cytokinin production by ove strains were found. The amount of cellular tRNA and the rate of cytokinin degradation were similar in ove and wild-type strains suggesting that the cause of over-production in the mutants may be due to changed control of a biosynthetic route independent of tRNA.

Electronic Resource

1435-1463

Springer Online Journal Archives 1860-2000

Medicine

Summary The enantiomers of LY141865, trans-(±)-4,4a,5,6,7,8a,9-octahydro-5-propyl-2H-pyrazolo [3,4-g]quinoline, were compared as dopamine D2 agonists by determining their abilities to elevate acetylcholine concentrations in rat corpus striatum. The levorotatory isomer, LY156258, increased striatal acetlycholine concentration at doses of 0.1–1 mg/kg i.p., whereas the dextrorotatory isomer had no effect even at doses as high as 30 mg/kg. The levorotatory isomer also decreased striatal concentrations of the dopamine metabolites, 3,4-dihydroxyphenylacetic acid and homovanillic acid, but did not significantly alter dopamine or 5-hydroxyindoleacetic acid concentration. The dextrorotatory isomer had no effect on any of these substances alone and did not alter the effects of the levorotatory isomer. The elevation of striatal acetylcholine levels by LY156258 was mimicked by pergolide, a dopamine agonist, and was totally prevented by pretreatment with haloperidol, a dopamine antagonist. The elevation of striatal acetylcholine concentration by LY157258 was maximal at 0.5 hour and declined thereafter, following a time course similar to that of pergolide. Neither LY141865 nor LY156258 shared with peroglide and dopamine the ability to activate striatal adenylate cyclasein vitro, an effect mediated by D1 receptors. LY141865 and LY156258 (but not the dextrorotatory isomer) inhibited the binding of tritiated apomorphine and spiperone to striatal membrane receptors, but were not as potent as pergolide, they also had less effect, or no effect, on the binding of other tritiated ligands (dopamine, WB4101, clonidine, dihydroalprenolol, pyrilamine or quinuclidinyl benzilate) to their membrane receptors. These results indicate that LY156258 stereospecifically activates dopamine D2 receptors and the studies are the first evidence of stereospecificity of dopamine receptors mediating an increase in striatal acetylcholine concentration.

Electronic Resource

1420-9071

Springer Online Journal Archives 1860-2000

Biology

Medicine

Summary The spontaneous conversion of mammalian cyclic GMP-dependent protein kinase (G-PK) into modulator-dependent protein kinase (type II) (M-PKII) in the absence of cGMP or histone was observed in vitro. The findings, together with similarity in substrate protein specificity, suggest that M-PKII is the catalytic subunit of mammalian G-PK.

Electronic Resource

0160-2527

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Psychology

Law

Electronic Resource

0019-7939

Economics

Labor Organizations

BOOK REVIEWS

1420-908X

Springer Online Journal Archives 1860-2000

Medicine

Abstract Cartilage which undergoes extensive autolysisin vitro (spontaneous or stimulated) is characterized by proteoglycan loss. Experimental conditions and inhibitor profils studies suggest neutral metalloproteinases induce the autolysis. In these preliminary studies we compared the degradation of Na2 35SO4 labeled bovine nasal cartilage (BNC) plugs placed in dialysis tubingin vitro andin vivo. The dialysis tubing was used to exclude large molecules (molecular weights greater than 2000) like proteinases, and proteinase inhibitors (e.g. α2-macroglobulin) but not potential test agents from the implanted cartilage. Cartilage autolysis occurred with live tissue but not with heat-killed tissue in both thein vitro andin vivo systems. In addition retinoic acid and phenanthroline were effective when placed inside or outside the dialysis tubing. A potentially useful procedure to evaluate agents which affect cartilage degradation is described.

Electronic Resource

1420-9071

Trans splicing ; spliced leader addition ; RNA editing

Springer Online Journal Archives 1860-2000

Biology

Medicine

Summary Members of the Trypanosomatidae, which include the African trypanosomes, the American trypanosomes and the leishmanias, cause disease in vast proportions in man and his livestock and are a major detrimental factor to the social and economic well-being of the third world. Current research using the techniques of molecular biology has revealed two unusual types of mRNA processing in these protozoans; these are the addition of a shared leader sequence to the 5′ ends of nuclear mRNAs by a mechanism oftrans splicing, and the insertion and deletion of specific uridine residues in mitochondrial transcripts by RNA editing. The presence of these two mRNA processing pathways in the Trypanosomatidae has profound consequences for the organization and expression of their genetic information.

Electronic Resource

1432-2072

Atypical antipsychotic ; Olanzapine ; Dopamine antagonist ; Dopamine ; Serotonin ; Muscarinic

Springer Online Journal Archives 1860-2000

Medicine

Abstract The ability of the atypical antipsychotic drug candidate olanzapine to antagonize dopamine, serotonin, α-adrenergic and muscarinic receptors in vivo was assessed by various neurochemical measurements in rat brain. Olanzapine increased the concentrations of the dopamine metabolites DOPAC and HVA in striatum and nucleus accumbens. Olanzapine antagonized the pergolide-induced decrease of striatal DOPA concentrations in rats treated with gammabutyrolactone and NSD1015 and increased striatal 3-methoxytyramine concentrations in nomifensine-treated rats (but not after gammabutyrolactone administration), suggesting that olanzapine blocked terminal and somatodendritic autoreceptors on dopamine neurons. Inactivation of dopamine D1 and D2 receptors by EEDQ was antagonized by olanzapine. The ex vivo binding of the 5HT2 radioligand [3H]-ketanserin was inhibited by olanzapine treatment, as was quipazine-induced increases in MHPG-SO4, evidence suggesting that olanzapine antagonized 5HT2 receptors. At higher doses, olanzapine increased the concentration of the norepinephrine metabolite, MHPG-SO4, probably by blocking α1-adrenergic receptors. Olanzapine inhibited ex vivo binding of the muscarinic antagonist radioligand [3H]-pirenzepine and lowered concentrations of striatal, but not hippocampal, acetylcholine levels. The findings provide evidence that olanzapine antagonized dopamine, serotonin, α-adrenergic and muscarinic receptors in vivo, consistent with its high affinity for these receptor sites in vitro.

Electronic Resource

1573-4803

Springer Online Journal Archives 1860-2000

Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics

Abstract Several mechanisms have been proposed to describe crack initiation and propagation in ductile-brittle composites. This experimental study shows that the failure of metal intermetallic (metal-aluminides) composites was initiated by cracking initiation in the intermetallic layers. For metal layers that allowed shear deformation, crack initiation in adjacent intermetallic layers resulted from shear bands propagating from a crack tip in the intermetallic layer through the metal layer and producing stress concentration points at the interfaces of adjacent intermetallic layers. For metal layers that did not support shear deformation, crack initiation in the intermetallic layers resulted from the continued build up of stresses within the intermetallic layers, resulting in a relatively uniform distribution of cracks within the individual intermetallic layers. Prior to failure, lateral constraints produce lateral cracks in the intermetallic layers. The final fracture features of both failure mechanisms were similar for both metal-intermetallic systems.

Electronic Resource

1432-2072

Key words A10 region ; Muscarinic receptor ; Microdialysis ; Mesocorticolimbic dopaminergic system

Springer Online Journal Archives 1860-2000

Medicine

Abstract  Objective: To investigate the function of muscarinic receptors in the ventral tegmental area in vivo, the release of endogenous monoamines was simultaneously measured in the somatodendritic (ventral tegmental area) and terminal (frontal cortex and nucleus accumbens) regions of the mesocorticolimbic dopaminergic system in rats, using dual probe microdialysis. Methods: Rats were implanted with dual microdialysis probes ipsilaterally into the ventral tegmental area (VTA) and nucleus accumbens (NAC) or frontal cortex (FC). Results: Intrategmental infusion of the muscarinic agonist oxotremorine M (OXO M, 0.1 and 1 mM) increased extracellular levels of dopamine and serotonin, but not noradrenaline, in the VTA to a maximum of 200% over baseline in both urethane-anaesthetized and unanaesthetized rats. In freely moving animals, this effect was accompanied by strong motor agitation. Both VTA dopamine and serotonin levels dropped to 60% or less of baseline when the perfusion medium was replaced by a calcium-free medium containing OXO M. In the NAC and FC, a similar increase in extracellular dopamine, but not serotonin and noradrenaline, was observed during OXO M infusion in the VTA. The removal of calcium during OXO M infusion in the VTA did not cause a decrease in NAC dopamine levels. Activation of serotonin and dopamine release by OXO M in the VTA and FC was dramatically reduced or prevented by the co-infusion of the muscarinic antagonist N-methylscopolamine (0.1 mM). Conclusion: These data demonstrate that VTA dopamine cells possess functional muscarinic receptors whose activation stimulates the release of dopamine in the VTA, NAC and FC. These results also suggest that muscarinic receptors may modulate the synaptic release of serotonin in the VTA.

Electronic Resource