Search Results - (Author, Cooperation:K. G. Skryabin)
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1X. Xu ; S. Pan ; S. Cheng ; B. Zhang ; D. Mu ; P. Ni ; G. Zhang ; S. Yang ; R. Li ; J. Wang ; G. Orjeda ; F. Guzman ; M. Torres ; R. Lozano ; O. Ponce ; D. Martinez ; G. De la Cruz ; S. K. Chakrabarti ; V. U. Patil ; K. G. Skryabin ; B. B. Kuznetsov ; N. V. Ravin ; T. V. Kolganova ; A. V. Beletsky ; A. V. Mardanov ; A. Di Genova ; D. M. Bolser ; D. M. Martin ; G. Li ; Y. Yang ; H. Kuang ; Q. Hu ; X. Xiong ; G. J. Bishop ; B. Sagredo ; N. Mejia ; W. Zagorski ; R. Gromadka ; J. Gawor ; P. Szczesny ; S. Huang ; Z. Zhang ; C. Liang ; J. He ; Y. Li ; Y. He ; J. Xu ; Y. Zhang ; B. Xie ; Y. Du ; D. Qu ; M. Bonierbale ; M. Ghislain ; R. Herrera Mdel ; G. Giuliano ; M. Pietrella ; G. Perrotta ; P. Facella ; K. O'Brien ; S. E. Feingold ; L. E. Barreiro ; G. A. Massa ; L. Diambra ; B. R. Whitty ; B. Vaillancourt ; H. Lin ; A. N. Massa ; M. Geoffroy ; S. Lundback ; D. DellaPenna ; C. R. Buell ; S. K. Sharma ; D. F. Marshall ; R. Waugh ; G. J. Bryan ; M. Destefanis ; I. Nagy ; D. Milbourne ; S. J. Thomson ; M. Fiers ; J. M. Jacobs ; K. L. Nielsen ; M. Sonderkaer ; M. Iovene ; G. A. Torres ; J. Jiang ; R. E. Veilleux ; C. W. Bachem ; J. de Boer ; T. Borm ; B. Kloosterman ; H. van Eck ; E. Datema ; B. Hekkert ; A. Goverse ; R. C. van Ham ; R. G. Visser
Nature Publishing Group (NPG)
Published 2011Staff ViewPublication Date: 2011-07-12Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Evolution, Molecular ; Gene Duplication ; Gene Expression Regulation, Plant ; Genes, Plant/genetics ; Genetic Variation ; Genome, Plant/*genetics ; *Genomics ; Haplotypes/genetics ; Heterozygote ; Homozygote ; Immunity, Innate ; Inbreeding ; Molecular Sequence Annotation ; Molecular Sequence Data ; Plant Diseases/genetics ; Ploidies ; Solanum tuberosum/*genetics/physiologyPublished by: -
2Hahn, K. D. ; Buck, F. ; Rüterjans, H. ; Chernov, B. K. ; Skryabin, K. G. ; Kirpichnikov, M. P.
Springer
Published 1985Staff ViewISSN: 1432-1017Keywords: O R 3 operator ; nuclear magnetic resonance ; assignment of proton resonances ; two-dimensional nuclear Overhauser effect spectroscopySource: Springer Online Journal Archives 1860-2000Topics: BiologyPhysicsNotes: Abstract The 17 base pair operator O R 3 oligonucleotide, which is the preferential binding site for the Cro repressor of phage λ, was studied by two-dimensional NMR spectroscopy. A sequential assignment procedure based on two-dimensional Nuclear Overhauser Effect (NOESY) and scalar coupling correlated (COSY) NMR spectroscopy, together with the knowledge of the oligodesoxynucleotide sequence, made it possible to assign the non-exhangeable base protons and the H1′ and the H2′-H2″ sugar protons of the O R 3 operator DNA. The pattern of the observed NOE connectivities is consistent with a right-handed helical DNA structure. The base and sugar proton assignments provide the necessary information for further studies of the O R 3 operator — Cro repressor interaction.Type of Medium: Electronic ResourceURL: -
3Vicente-Carbajosa, J. ; Beritashvili, D. R. ; Kraev, A. S. ; Skryabin, K. G.
Springer
Published 1992Staff ViewISSN: 1573-5028Keywords: B hordein genes ; barley ; PCR ; Southern blot analysisSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract This work presents new information on the structure and organization of B hordein genes in the Hor 2 locus of barley. Data obtained by Southern blot analysis and cloning and sequencing of different members of this multigene family are discussed.Type of Medium: Electronic ResourceURL: -
4Expression of env sequences of the bovine leukemia virus (BLV) in the yeast Saccharomyces cerevisiaeBrantl, S. ; Eldarov, M. A. ; Rößler, H. ; Drescher, B. ; Lang, H. ; Rosenthal, S. ; Skryabin, K. G.
New York, NY [u.a.] : Wiley-Blackwell
Published 1988Staff ViewISSN: 0749-503XKeywords: Bovine leukemia virus ; PH05 ; PGK ; tumor virus ; Saccharomyces cerevisiae ; viral antigens ; Life and Medical Sciences ; GeneticsSource: Wiley InterScience Backfile Collection 1832-2000Topics: BiologyNotes: DNA sequences of the envelop (env) gene of the bovine leukemia virus (BLV) were expressed in the yeast saccharomyces cerevisiae. Two yeast promoters, the responsible PH05 promoter and the constitutive PGK promoter, were used to construct four expression plasmids either a sequence of the surface antigen gp51 or a (gp51 + gp30) sequence.The expressed hetrologous gene products were characterized by Western blot analysis and competitive radio-immunoassay. By means of Northern blot analysis the steady-state level of env-specific mRNA was analysed.The highest expression rate was obtained from recombinant plasmid YEpSG 94 comprising a gp51 sequence - a 630 base pair fragment containing 70% of the gp51 but lacking the N terminus - as well as the PH05 promoter including PH05 signal sequence and the PH05 terminator. The recombinant gp51 was partially lycosylated but the PH05 signal peptide did not seem to be cleaved off. No immunoreactive material could be found in the periplasm or in the culture medium.By means of monoclonal antibodies directed against eight different epitopes of viral gp51, all for sequential antigenic determinants were detected in the AH 216(YEpSG 94) expression product.Additional Material: 7 Ill.Type of Medium: Electronic ResourceURL: