Search Results - (Author, Cooperation:K. D. Wittrup)
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1Latest Papers from Table of Contents or Articles in PressGreen, D. J., OSteen, S., Lin, Y., Comstock, M. L., Kenoyer, A. L., Hamlin, D. K., Wilbur, D. S., Fisher, D. R., Nartea, M., Hylarides, M. D., Gopal, A. K., Gooley, T. A., Orozco, J. J., Till, B. G., Orcutt, K. D., Wittrup, K. D., Press, O. W.
American Society of Hematology (ASH)
Published 2018Staff ViewPublication Date: 2018-02-09Publisher: American Society of Hematology (ASH)Print ISSN: 0006-4971Electronic ISSN: 1528-0020Topics: BiologyMedicineKeywords: Multiple Myeloma, Immunobiology and Immunotherapy, Lymphoid NeoplasiaPublished by: -
2Latest Papers from Table of Contents or Articles in PressZ. A. Bornholdt ; H. L. Turner ; C. D. Murin ; W. Li ; D. Sok ; C. A. Souders ; A. E. Piper ; A. Goff ; J. D. Shamblin ; S. E. Wollen ; T. R. Sprague ; M. L. Fusco ; K. B. Pommert ; L. A. Cavacini ; H. L. Smith ; M. Klempner ; K. A. Reimann ; E. Krauland ; T. U. Gerngross ; K. D. Wittrup ; E. O. Saphire ; D. R. Burton ; P. J. Glass ; A. B. Ward ; L. M. Walker
American Association for the Advancement of Science (AAAS)
Published 2016Staff ViewPublication Date: 2016-02-26Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyComputer ScienceMedicineNatural Sciences in GeneralPhysicsPublished by: -
3Articles: DFG German National LicensesWITTRUP, K. D. ; ROBINSON, A. S. ; PAREKH, R. N. ; FORRESTER, K. J.
Oxford, UK : Blackwell Publishing Ltd
Published 1994Staff ViewISSN: 1749-6632Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: Natural Sciences in GeneralType of Medium: Electronic ResourceURL: -
4Articles: DFG German National LicensesWittrup, K. D. ; Mann, M. B. ; Fenton, D. M. ; Tsai, L. B. ; Bailey, J. E.
[s.l.] : Nature Publishing Company
Published 1988Staff ViewISSN: 1546-1696Source: Nature Archives 1869 - 2009Topics: BiologyProcess Engineering, Biotechnology, Nutrition TechnologyNotes: [Auszug] Accumulation of foreign proteins as inclusion bodies in Escherichia coli was investigated using single–cell light scatter measurements from flow cytometry. Significant increases in forward–angle light scatter and right–angle light scatter were observed for E. coli strains ...Type of Medium: Electronic ResourceURL: -
5Articles: DFG German National LicensesStaff View
ISSN: 1432-0797Source: Springer Online Journal Archives 1860-2000Topics: Process Engineering, Biotechnology, Nutrition TechnologyNotes: Abstract The effects of dissolved oxygen concentration on hybridoma cell growth, metabolism, and antibody production were studied. A mouse hybridoma cell line producing an IgG1 directed at a consensus α-interferon was grown in batch cultures in a 5 dm3 stirred bioreactor at dissolved oxygen (DO) concentrations of 5, 30, 90 and 95% or air saturation. High oxygen tension (95% of air saturation) reduced specific growth rate without affecting cell viability. At lower dissolved oxygen levels, specific growth rates were approximately independent of DO, although changes in mitochondrial function and antibody production were observed. Flow cytometry assays of a fluorescent mitochondria-specific marker (Rhodamine 123) show significant single-cell heterogeneities during late exponential growth and greater average fluorescence in cultures grown at 95% DO. The quantity of cell-surface immunoglobulin, measured by an immunofluorescent flow cytometric technique, was the same at high (95%) and low (5%) dissolved oxygen concentrations. Myeloma cells of the type used in constructing the above hybridoma line were much less sensitive to dissolved oxygen level. Specific respiration rate, pyruvate utilization rate, cytochrome oxidase activity, and succinate-cytochrome c oxidoreductase activity were significantly greater (62–116%) for the hybridoma cells than for the myeloma cells in T-flask cultures.Type of Medium: Electronic ResourceURL: -
6Articles: DFG German National LicensesStaff View
ISSN: 1573-6784Source: Springer Online Journal Archives 1860-2000Topics: Process Engineering, Biotechnology, Nutrition TechnologyNotes: Summary The two most commonly used amino acid media supplements do not improve the specific rate of secretion of Schizosaccharomyces pombe acid phosphatase in S. cerevisiae relative to unsupplemented Synthetic Dextrose (SD) media. New amino acid supplement formulations have been obtained which produce an 8-fold increase in total phosphatase secretion, and a 70% increase in specific phosphatase secretion.Type of Medium: Electronic ResourceURL: -
7Articles: DFG German National LicensesStaff View
ISSN: 0006-3592Keywords: Chemistry ; Biochemistry and BiotechnologySource: Wiley InterScience Backfile Collection 1832-2000Topics: BiologyProcess Engineering, Biotechnology, Nutrition TechnologyNotes: A segregated model of multicopy plasmid propagation has been formulated which incorporates plasmid replication and partition functions, as well as the effect of plasmid presence on host growth rate. Growth of plasmid-free cells in selective medium is explicitly analyzed. The model parameters can be determined from experimentally measurable quantities. Propagation of a recombinant multicopy plasmid in the yeast Saccharomyces cerevisiae is analyzed using this model.Additional Material: 6 Ill.Type of Medium: Electronic ResourceURL: -
8Articles: DFG German National LicensesStaff View
ISSN: 0006-3592Keywords: microencapsulation ; selection ; secretion ; yeast ; Chemistry ; Biochemistry and BiotechnologySource: Wiley InterScience Backfile Collection 1832-2000Topics: BiologyProcess Engineering, Biotechnology, Nutrition TechnologyNotes: We have developed a microencapsulation selection method which allows the rapid and quantitative screening of 〉106 yeast cells for enhanced secretion of Aspergillus awamori glucoamylase. The method provides a 400-fold single-pass enrichment for high-secreting mutants, and can be straightforwardly adapted for application to growth-based selection schemes with other microorganisms and enzymes. © 1993 John Wiley & Sons, Inc.Additional Material: 2 Ill.Type of Medium: Electronic ResourceURL: -
9Articles: DFG German National LicensesStaff View
ISSN: 0006-3592Keywords: Chemistry ; Biochemistry and BiotechnologySource: Wiley InterScience Backfile Collection 1832-2000Topics: BiologyProcess Engineering, Biotechnology, Nutrition TechnologyType of Medium: Electronic ResourceURL: