Search Results - (Author, Cooperation:J. Storz)
-
1Latest Papers from Table of Contents or Articles in PressJ. Aleksic ; S. Ansoldi ; L. A. Antonelli ; P. Antoranz ; A. Babic ; P. Bangale ; J. A. Barrio ; J. Becerra Gonzalez ; W. Bednarek ; E. Bernardini ; B. Biasuzzi ; A. Biland ; O. Blanch ; S. Bonnefoy ; G. Bonnoli ; F. Borracci ; T. Bretz ; E. Carmona ; A. Carosi ; P. Colin ; E. Colombo ; J. L. Contreras ; J. Cortina ; S. Covino ; P. Da Vela ; F. Dazzi ; A. De Angelis ; G. De Caneva ; B. De Lotto ; E. de Ona Wilhelmi ; C. Delgado Mendez ; D. Dominis Prester ; D. Dorner ; M. Doro ; S. Einecke ; D. Eisenacher ; D. Elsaesser ; M. V. Fonseca ; L. Font ; K. Frantzen ; C. Fruck ; D. Galindo ; R. J. Garcia Lopez ; M. Garczarczyk ; D. Garrido Terrats ; M. Gaug ; N. Godinovic ; A. Gonzalez Munoz ; S. R. Gozzini ; D. Hadasch ; Y. Hanabata ; M. Hayashida ; J. Herrera ; D. Hildebrand ; J. Hose ; D. Hrupec ; W. Idec ; V. Kadenius ; H. Kellermann ; K. Kodani ; Y. Konno ; J. Krause ; H. Kubo ; J. Kushida ; A. La Barbera ; D. Lelas ; N. Lewandowska ; E. Lindfors ; S. Lombardi ; F. Longo ; M. Lopez ; R. Lopez-Coto ; A. Lopez-Oramas ; E. Lorenz ; I. Lozano ; M. Makariev ; K. Mallot ; G. Maneva ; N. Mankuzhiyil ; K. Mannheim ; L. Maraschi ; B. Marcote ; M. Mariotti ; M. Martinez ; D. Mazin ; U. Menzel ; J. M. Miranda ; R. Mirzoyan ; A. Moralejo ; P. Munar-Adrover ; D. Nakajima ; A. Niedzwiecki ; K. Nilsson ; K. Nishijima ; K. Noda ; R. Orito ; A. Overkemping ; S. Paiano ; M. Palatiello ; D. Paneque ; R. Paoletti ; J. M. Paredes ; X. Paredes-Fortuny ; M. Persic ; J. Poutanen ; P. G. Prada Moroni ; E. Prandini ; I. Puljak ; R. Reinthal ; W. Rhode ; M. Ribo ; J. Rico ; J. Rodriguez Garcia ; S. Rugamer ; T. Saito ; K. Saito ; K. Satalecka ; V. Scalzotto ; V. Scapin ; C. Schultz ; T. Schweizer ; S. N. Shore ; A. Sillanpaa ; J. Sitarek ; I. Snidaric ; D. Sobczynska ; F. Spanier ; V. Stamatescu ; A. Stamerra ; T. Steinbring ; J. Storz ; M. Strzys ; L. Takalo ; H. Takami ; F. Tavecchio ; P. Temnikov ; T. Terzic ; D. Tescaro ; M. Teshima ; J. Thaele ; O. Tibolla ; D. F. Torres ; T. Toyama ; A. Treves ; M. Uellenbeck ; P. Vogler ; R. Zanin ; M. Kadler ; R. Schulz ; E. Ros ; U. Bach ; F. Krauss ; J. Wilms
American Association for the Advancement of Science (AAAS)
Published 2014Staff ViewPublication Date: 2014-11-08Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyComputer ScienceMedicineNatural Sciences in GeneralPhysicsPublished by: -
2Articles: DFG German National LicensesStorz, J. ; Collier, J. R. ; Eugster, A. K. ; Altera, K. P.
Oxford, UK : Blackwell Publishing Ltd
Published 1971Staff ViewISSN: 1749-6632Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: Natural Sciences in GeneralType of Medium: Electronic ResourceURL: -
3Articles: DFG German National LicensesStaff View
ISSN: 1476-4687Source: Nature Archives 1869 - 2009Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsNotes: [Auszug] THE cell-free synthesis of infectious ribonucleic acid (UNA) of tobacco mosaic virus (TMV) using extracts from virus-infected plant cells has been reported1"7. Recently we recorded that extracts from normal plant cells apparently synthesized infectious TMV UNA when primed with either ...Type of Medium: Electronic ResourceURL: -
4Articles: DFG German National LicensesStaff View
ISSN: 0022-5320Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyType of Medium: Electronic ResourceURL: -
5Articles: DFG German National LicensesStaff View
ISSN: 0093-691XSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: MedicineType of Medium: Electronic ResourceURL: -
6Articles: DFG German National LicensesStaff View
ISSN: 1432-8798Source: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Summary A hemadsorbing enteric virus (HADEN), isolated from the gastrointestinal tract of cattle and previously classified as a bovine enterovirus, was found to induce Feulgen-positive intranuclear inclusions and to be inhibited in its replication by FUDR, BUDR and actinomycin D, indicating DNA as genetic material. Because of this fact and the other known properties of HADEN virus it should be considered a bovine parvovirus.Type of Medium: Electronic ResourceURL: -
7Articles: DFG German National LicensesStaff View
ISSN: 1432-8798Source: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Summary Hemagglutinating and acetylesterase functions as well as the 124 kDa glycoprotein were present in the highly cell-culture adapted, avirulent bovine coronavirus strain BCV-L9, in the Norden vaccine strain derived from it, and in 5 wild-type, virulent strains that multiplied in HRT-18 cells but were restricted in several types of cultured bovine cells. The BCV-L9 and the wild-type strain BCV-LY-138 agglutinated chicken and mouse erythrocytes. The acetylesterase facilitated break-down of the BCV-erythrocyte complex with chicken but only to a minimal extent with mouse erythrocytes in the receptor-destroying enzyme test. Purified preparations of the vaccine and the wild-type strains agglutinated chicken erythrocytes at low titers and mouse erythrocytes at 128 to 256 times higher titers whereas receptor destroying enzyme activity was detectable only with chicken erythrocytes. When wild-type strains were propagated in HRT cells at low passage levels, they produced 5×105 to 4.5×106 plaque forming units per 50 µl which agglutinated erythrocytes from mice but not from chickens. Diisopropylfluoro-phosphate moderately increased the hemagglutination titers, but completely inhibited the receptor destroying enzyme of purified virus of all strains. It had virtually no influence on the plaque-forming infectivity of the different BCV strains. The acetylesterase of strain BCV-L9 reacting in the receptor-destroying enzyme test was stable for 3h at 37 and 42°C. It was inactivated within 30 min at 56°C while the hemagglutinin function of this strain was stable for 3 h at 37, 42, and 56°C, but it was inactivated at 65°C within 1 h.Type of Medium: Electronic ResourceURL: -
8Articles: DFG German National LicensesStaff View
ISSN: 1432-8798Source: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Summary Tritiated uridine was selectively incorporated into the cytoplasm but not the nucleus of BEK cells infected with bovine enterovirus. This was accompanied by complete shutdown of cellular RNA synthesis. Bovine embryonic spleen cells infected with the BVD virus incorporated3H-uridine into the nucleus and cytoplasm, while uninfected cells showed only nuclear and nucleolar incorporation. Bovine embryonic spleen cells that were preinfected with BVD virus for 20 hours and then superinfected with bovine enterovirus showed complete absence of nuclear and nucleolar labelling in the early stages of superinfection with the bovine enterovirus, but nuclear labelling returned in reduced amounts 8 to 10 hours after superinfection and persisted throughout the remainder of the double infection ending in cell death.Type of Medium: Electronic ResourceURL: -
9Articles: DFG German National LicensesStaff View
ISSN: 1432-8798Source: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Summary Polykaryon formation in bovine fetal spleen (BFS) cells infected with bovine coronavirus L9 occurred only in media supplemented with trypsin. A single 1 to 2 h trypsin treatment 10 h and later after infection induced formation of polykaryons. Trypsin treatment at pH 7.5 and 8.0 induced polykaryons while treatments at lower or higher pH levels did not. Cell fusion activity was partially suppressed by the presence of antibody.Type of Medium: Electronic ResourceURL: -
10Articles: DFG German National LicensesStaff View
ISSN: 1432-8798Source: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Summary The polypeptide profile of the cell-adapted strain of bovine coronavirus (Mebus BCV-L9) is remarkably affected by the host cell and trypsin. We compared the structural proteins of virus purified from different cell lines and found cell-dependent differences in the virus structure. BCV was purified from four clones of human rectal tumour cells (HRT-18): 3 F3, D 2, 3 E 3, and 4 B 3. The structural profiles of BCV propagated in clones 3 E 3 and 3 F 3 were identical, consisting of proteins with molecular weights of 185, 160, 140, 125, 110, 100, 52, 46, 37, 31–34, and 26–28 kilodaltons (kd). BCV purified from clone D2 lacked the 100 kd species, and clone 4 B 3 yielded virus lacking the 46 kd protein. We compared the structures of BCV propagated in HRT-18 cells [BCV(HRT-18)] and virus raised in bovine fetal spleen cells [BCV(D 2 BFS)]. The concentration of the 185 kd protein was higher in BCV (D 2BFS), and it also contained a 200 kd species. Protein profiles of in vitro trypsin treated and untreated BCV(HRT-18) differed only under reducing conditions, suggesting that trypsin cleavage sites are located within disulfide-linked regions of affected proteins. Propagation of BCV in D 2 BFS cells in the presence of trypsin resulted in cleavage of the 185 kd protein and a concommitant increase of the 100 kd protein. Activation of the fusion function probably depends on this cleavage process because fusion of BCV-infected D 2 BFS cells is trypsin dependent.Type of Medium: Electronic ResourceURL: -
11Articles: DFG German National LicensesStaff View
ISSN: 1432-8798Source: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Summary The early events in the infection of human rectal tumor cells by bovine coronavirus were investigated by colloidal gold-mediated immunoelectron microscopy and by analysis of the effect of lysosomotropic weak bases on virus yield. Electron microscopic studies revealed sites of fusion between the virus envelope and the plasmalemma but fusion events along intracellular membranes were not observed despite extensive searches. Virion-antibody-colloidal gold complexes were, in fact, endocytosed by synchronously infected cells. These complexes were apparently non-infectious, and they accumulated in vacuoles that resembled secondary lysosomes. Exposure of cells to ammonium chloride or to methylamine during the first hour of infection had little inhibitory effect on the production of infectious virus. Chloroquine treatments were inhibitory but this effect depended on relatively late events in the infectious process. The chloroquine inhibitory step blocked infection of virus adsorbed to cells that were exposed to buffers in the pH range of 4.4 to 8.4. These findings indicate that BCV penetrates its host cell by direct fusion with the plasmalemma and does not require an acidic intracellular compartment for infectious entry.Type of Medium: Electronic ResourceURL: -
12Articles: DFG German National LicensesStaff View
ISSN: 1432-8798Source: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Summary The bovine coronavirus strain LY-138 was purified by differential as well as velocity and isopycnic centrifugation in sucrose or CsCl gradients. The substrate for purification was contents of the small intestine of experimentally inoculated calves. This strain is highly enteropathogenic, but it could not yet be propagated in cultured cells. Intact virions had a density of 1.245 g/cm3 in CsCl and 1.185 g/cm3 in sucrose. A spherical core-like structure with an average diameter of 82 nm remaining after treatment with chloroform had a density of 1.299 g/cm3 in CsCl and 1.201 g/cm3 in sucrose. Seven distinct bands of polypeptides and 4 shoulders were detected after electrophoresis of SDS-solubilized virions in polyacrylamide gels. The approximate molecular weights ranged from 110,000 to 36,000. Four of the bands gave a PAS positive reaction. These 4 glycoproteins and an additional protein with an approximate molecular weight of 70,000 were removed by chloroform treatment. The remaining core-like structure contained the 2 polypeptides VP3 and VP7.Type of Medium: Electronic ResourceURL: -
13Articles: DFG German National LicensesStaff View
ISSN: 1432-8798Source: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Summary The nucleotide sequence of the S gene of the bovine respiratory coronavirus (BRCV) strain G95, which was isolated from nasal swabs of a calf suffering from respiratory disorders, was determined and compared with the S gene of the enteropathogenic bovine coronavirus (BECV) strain LY138. Sequence analysis revealed 98.7% nucleotide and 98.3% deduced amino acid identities between the S genes of BRCV-G95 and BECV-LY138 without any deletions or insertions. Nucleotide substitutions were distributed randomly throughout the gene. Five monoclonal antibodies specific for the S protein distinguished BRCV-G95 from BECV-L9, but failed to differentiate it from BECV-LY138 in Western blots under denatured and native conditions. BRCV-G95 induced cytopathic changes in cell cultures that were similar to BECV-LY138 but different from BECV-L9. These results suggest that strain BRCV-G95 is more closely related to the virulent strain BECV-LY138 than to the avirulent, cell culture-adapted strain BECV-L9.Type of Medium: Electronic ResourceURL: -
14Articles: DFG German National LicensesStaff View
ISSN: 1432-8798Source: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Summary. Antibody responses against respiratory bovine coronavirus (RBCV) infections were monitored in cattle from the onset of a naturally occurring severe shipping fever (SF) epizootic to complete recovery of affected cattle or fatal outcomes. The infection with RBCV was detected in nasal secretions of 86 cattle, and 81 of them developed acute respiratory tract disease, including fatal pneumonia. Cattle nasally shedding RBCV at the beginning of the epizootic experienced characteristic primary immune responses with specific antibodies for hemagglutinin-esterase (HE) and spike (S) glycoproteins. Virus shedding in nasal secretions of the majority of the cattle ceased between days 7 and 14 with the appearance of HE- and S-specific antibodies. Nasal samples and lung tissues from 9 of the 10 fatal cases had high titers of RBCV, but these cattle had only IgM responses to RBCV infections. Cattle remaining negative in RBCV isolation tests entered this epizootic with antibodies against HE and S. Protection against respiratory tract disease was apparently associated with high level of opsonic and virus-neutralizing IgG2. The HE and S glycoproteins were recognized earliest by the bovine immune system while the N protein induced antibody responses during the later stage of initial infection and the early stage of reinfection. The membrane (M) glycoprotein was the least immunogenic of the major viral structural proteins.Type of Medium: Electronic ResourceURL: -
15Articles: DFG German National LicensesStaff View
ISSN: 1432-8798Source: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Summary One-step growth curves of a bovine enterovirus and the virus of bovine diarrhea (BVD) in singly and doubly infected bovine embryonic kidney (BEK) and bovine embryonic spleen (BES) cells were determined. In BEK cells, bovine enterovirus infectivity increased exponentially in both tissue culture fluid and cells 4 to 10 hours after inoculation and then levelled off. In BES cells, the exponential increase lasted 14 to 17 hours. In the growth curve of the BVD virus in BEK cells, the extracellular and cell-associated viruses increased exponentially until hour 12, increasing gradually thereafter. In BES cells, BVD virus infectivity increased exponentially 24 hours after infection and then levelled off. The peak infectivity in BES cells was reached 24 hours before that in BEK cells. Growth of bovine enterovirus was studied in cells of BEK and BES monolayers preinfected with the BVD virus for 20 hours. The infectivity titer in preinfected BEK cells was approximately 2 logs less than in preinfected BES cells. A decreased yield in infectivity was observed compared to the bovine enterovirus one-step growth curve.Type of Medium: Electronic ResourceURL: -
16Articles: DFG German National LicensesStaff View
ISSN: 1432-1831Source: Springer Online Journal Archives 1860-2000Topics: MedicineDescription / Table of Contents: Zusammenfassung 5 identische Virusisolate wurden aus fetalen bovinen Milzzellen gewonnen, die 30–67 Tage in Kultur gehalten worden waren. Während dieser Zeit wurden die Zellen 3–8 mal subkultiviert. Die Zellkulturen anderer Organe von 3 Spenderfeten zeigten keine Hinweise einer lytischen Virusinfection. Die Virusisolate wurden morphologisch, biologisch und durch Charakterisierung ihrer Virus-DNS als Herpesvirus der Infektiösen Bovinen Rhinotracheitis identifiziert. Die erfaßbaren Daten deuten darauf hin, daß das Virus in latenter Form in den Spenderfeten vorhanden war.Notes: Abstract Five identical viral isolates were recovered from bovine fetal spleen cells maintained in culture for 30 to 67 days. During this time the cells were sub-cultured 3 to 8 times. Cells cultured from other organs of the 3 donor fetuses did not evidence lytic viral infections. The viral isolates were identified serologically, morphologically, culturally and by characterization of the virus DNA as the herpesvirus of infectious bovine rhinotracheitis. The available data indicate latent infection of the donor fetuses as the source of these viral isolates.Type of Medium: Electronic ResourceURL: -
17Articles: DFG German National LicensesStaff View
ISSN: 1432-1831Source: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Abstract Antibodies in human serum against an enteropathogenic bovine coronavirus were detected by double immunodiffusion (DID), neutralization of infectivity, indirect immunofluorescence, and immune electron microscopy. Human sera reacting in the DID test neutralized the infectivity of the bovine coronaviruses to indices of 2.5 to 〉 5. Nineteen of 40 DID-negative, heat-inactivated sera had neutralizing indices of 1 to 3.0. Human serum with neutralizing and DID antibodies produced juxtanuclear and cytoplasmic fluorescence identical to that of bovine immune serum in cells infected with the bovine coronavirus. Antibodies in human and bovine sera interacted with the peplomeres of the bovine coronavirus, matting and bridging them, when present in excess, and facilitated formation of large viral aggregates when present in equivalent concentrations. Complement added to the virus-antibody complexes did not alter specifically the morphology of single, antibody-laden viral particles or viral particles in aggregates. Evidence of the transmission of coronavirus from experimentally inoculated calves to man, with ensuing gastroenteritis, was found by electron microscopic tracing of the coronavirus and its virus-antibody complexes.Type of Medium: Electronic ResourceURL: