Search Results - (Author, Cooperation:J. R. Patel)

2015-09-30

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

2015-01-22

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Amino Acids/*chemical synthesis/chemistry/metabolism/*pharmacology ; Amino Acyl-tRNA Synthetases/genetics/metabolism ; Catalytic Domain/genetics ; Codon/genetics ; Containment of Biohazards/*methods ; Culture Media/chemistry/pharmacology ; Environment ; Escherichia coli/cytology/*drug effects/*genetics/metabolism ; Escherichia coli Proteins/biosynthesis/chemistry/genetics/metabolism ; Evolution, Molecular ; Gene Transfer, Horizontal/genetics ; Genes, Essential/genetics ; Genetic Code/genetics ; Genetic Engineering/methods ; Genome, Bacterial/genetics ; Microbial Viability/*drug effects/genetics ; Molecular Sequence Data ; Organisms, Genetically Modified/genetics/growth & development/metabolism ; Peptide Termination Factors/genetics ; Phenylalanine/chemistry/metabolism ; Protein Multimerization/genetics ; RNA, Transfer/genetics ; Synthetic Biology/*methods

2018-12-11

The American Association of Immunologists (AAI)

0022-1767

1550-6606

Medicine

1077-3118

AIP Digital Archive

Physics

The position of As atoms on the silicon(100) surface has been determined using the x-ray standing-wave technique and synchrotron radiation. Since the experiments were performed in air, the As-covered (100) surface was capped with amorphous silicon. The results of the measurements are in agreement with the symmetric As dimer model for the (100) surface. The distance of the As dimers normal to the ideal (100) surface was found to be 1.26±0.01 A(ring) which agrees with the value obtained from total energy minimization calculations. With no protective coating the As position was higher.

Electronic Resource

1476-4687

Nature Archives 1869 - 2009

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

[Auszug] Stormont et l.6 have demonstrated the homology of nine genetic systems of cattle blood groups with those of bison. Owen et al. were unable to demonstrate clearly definite differences between cattle and bison blood groups by means of cross-absorptions of hetero-immune (rabbit) antisera. This ...

Electronic Resource

1432-8798

Springer Online Journal Archives 1860-2000

Medicine

Summary Subtype-1 isolates of Equine herpesvirus-1 (EHV-1) from a quadriplegic horse and from an aborted foetus were compared with each other and with a subtype-2 respiratory isolate. All 3 isolates were detected in the epithelium and macrophages of the respiratory tract. Both the paresis and foetal subtype-1 isolates replicated in the epithelium of the ileum and this correlated with the recovery of virus from faecesin vivo. The paresis subtype-1 isolate also had a predelection for vascular endothelial cells, particularly in the nasal mucosa, but also in the lungs, central nervous system, adrenal and thyroid. In the 9 foals inoculated with the paresis isolate two developed hind limb dysfunction, four developed diarrhoea, and one of these 4 died with an intussusception. The differences between these isolates are discussed in relation to other herpesviruses.

Electronic Resource

1432-8798

Springer Online Journal Archives 1860-2000

Medicine

Summary Eight mares were infected with equid herpesvirus-1 subtype 1 isolated from a case of equine paresis. In two mares killed at 4 d.p.i. immunofluorescence showed endothelial cell infection together with thrombosis in the rete arteriosus of the nasal mucosa and also in the spinal cord of one of these mares. Circulating platelet counts in the other six mares fell as early as 2 d.p.i. and remained depressed for seven days. Circulating immune complexes started to appear at 2 d.p.i., reached maximum levels at 10 d.p.i., but were undetectable at 28 d.p.i. Three of the six remaining mares developed varying degrees of inco-ordination at 8 and 9 d.p.i. In the two inco-ordinate mares that were killed at 9 and 10 d.p.i. the haemorrhages in the spinal cord and brain were associated with extensive endothelial cell fluorescence and thrombus formation. Clinical paresis coincided with an increase in circulating complement fixing and neutralising antibodies which in all six mares were higher against the subtype 2 isolate than subtype 1. In five yearlings infected with a subtype 2 isolate of EHV-1 platelet counts remained normal and neither immune complexes nor viraemia, nor inco-ordination were detected.

Electronic Resource

1432-8798

Springer Online Journal Archives 1860-2000

Medicine

Summary Lymphoproliferative disease virus of turkeys (LPDV), a C-type retrovirus, was shown to contain 3 major [32 kilodaltons (kd, p 32), 26 kd, 22/21 kd] and 2 minor (41 kd and 12 kd) polypeptides. Preliminary evidence suggests a glycoprotein of 76 kd (GP 76) and a major doublet polypeptide of 13.5/13 kd to be also of viral origin. Of these GP 76 was susceptible to bromelain action implying its surface location in the virion, while p 32, p 26 and p 13.5/13 were the main constituents of viral cores. p 13.5/13 bound an RNA probe, suggesting it to be the main constituent of viral ribonucleoprotein. p 22/21 was not cleaved by bromelain, and was absent in viral cores suggesting its intramembrane location between virion envelope and core. The polypeptide profile of LPDV is distinct from those of avian sarcomaleukosis viruses and avian reticuloendotheleosis viruses.

Electronic Resource

1432-8798

Springer Online Journal Archives 1860-2000

Medicine

Summary In calves as in rabbits MCFV infectivity was detected in differentiated lymphocytes of 11 to 13 µm diameter and not in the proliferating lymphoblastoid cells. The increase in the number of infectious lymphocytes on explant was less in cattle than in rabbits but the initial infectivity was higher. Explanations for these disparities are suggested.

Electronic Resource

1432-8798

Springer Online Journal Archives 1860-2000

Medicine

Summary A tissue-culture-grown enteric coronavirus infected the whole of the gastrointestinal tract and caused enteritis and diarrhoea in a 10-day-old gnotobiotic Friesian calf. Diarrhoea occurred 2 days after inoculation and excretion of virus in faeces and rectal swabs increased until necropsy at 3 days. Virus growth, as detected by indirect immunofluorescence, virus isolation, and thin section electron microscopy, was most extensive in the epithelium of the colon and rectum at 3 days after inoculation. No virus was detected in other organs or in the pleural, peritoneal and oropharyngeal cavities. The origin and host specificity of this coronavirus is discussed.

Electronic Resource

1573-4811

Springer Online Journal Archives 1860-2000

Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics

Electronic Resource

0006-3525

Chemistry ; Polymer and Materials Science

Wiley InterScience Backfile Collection 1832-2000

Chemistry and Pharmacology

Intrinsic viscosity and specific optical rotation measurements have been carried out for Carboxymethyl amylose (CMA) solutions in 0.15M sodium chloride at various pH values. Potentiometric titrations of CMA have been performed at various polymer concentrations as well as in presence of different divalent cations. The amount of divalent cation (Ni+2) bound to CMA has been determined by using a new method af analysis based on polyelectrolyte theory. Finally from the results it is shown that CMA may exist as a random coil in solution.

7 Ill.

Electronic Resource

0025-116X

Chemistry ; Polymer and Materials Science

Wiley InterScience Backfile Collection 1832-2000

Chemistry and Pharmacology

Physics

Die Viskosität verdünnter Lösungen von fünf Fraktionen der Natriumcarboxymethylamylose (NaCMA) wurde bei Temperaturen zwischen 25 und 62,5°C bestimmt. Werte für die scheinbare Aktivierungsenergie des Viskositätsflusses Q und der präexponentielle Therm A wurden auf Grund der Beziehung \documentclass{article}\pagestyle{empty}\begin{document}$$\eta = Ae^{{Q \mathord{\left/ {\vphantom {Q {RT}}} \right. \kern-\nulldelimiterspace} {RT}}} $$\end{document} erhalten. Die Abhängigkeit von Q und A von der Konzentration c wurde gemäß den Gleichungen \documentclass{article}\pagestyle{empty}\begin{document}$$ Q = Q_0 + K_e Mc $$\end{document} \documentclass{article}\pagestyle{empty}\begin{document}$${\rm and }A = A_0 \exp \left( { - K'_e M^\alpha c} \right) $$\end{document} diskutiert, wobei Q0 und A0 die Werte für das Lösungsmittel und Ke, Ka′ und α die Parameter darstellen, die von der Natur des Polymeren und des Lösungsmittels abhängen. Aus diesen Parametern wird geschlossen, daß NaCMA-Ketten steif sind. Die HUGGINSkonstante k′ wird in 0,03, 0,12 und 0,25 m NaCl-Lösungen bestimmt und ihre Abhängigkeit von der Güte des Lösungsmittels und der Temperatur diskutiert.

Viscosities of dilute solutions of five fractions of sodium carboxymethyl amylose (NaCMA) have been determined at different temperatures between 25 and 62.5°C. Values of the apparent activation energy of viscous flow Q and the pre-exponential term A have been obtained using the relationship \documentclass{article}\pagestyle{empty}\begin{document}$$ \eta = Ae^{{Q \mathord{\left/ {\vphantom {Q {RT}}} \right. \kern-\nulldelimiterspace} {RT}}} $$\end{document} Variation of Q and A with polymer concentration c has been discussed according to the equations \documentclass{article}\pagestyle{empty}\begin{document}$$ Q = Q_0 + K_e Mc $$\end{document} \documentclass{article}\pagestyle{empty}\begin{document}$${\rm and }A = A_0 \exp \left( { - K'_e M^\alpha c} \right) $$\end{document} where Q0 and A0 are the values for the solvent, Ke, K'a and α are the parameters which depend on the nature of the polymer and solvent. From these parameters it is concluded that NaCMA chains are stiff chains. The HUGGINS constant k' is determined in 0.03, 0.12, and 0.25 M NaCl solutions and its variation with solvent power and temperature is discussed.

5 Ill.

Electronic Resource

0025-116X

Chemistry ; Polymer and Materials Science

Wiley InterScience Backfile Collection 1832-2000

Chemistry and Pharmacology

Physics

Es wurde gefunden, daß die von FLORY-FOX-SCHAEFGEN angegebene Beziehung zur Bestimmung der ungestörten Dimensionen auf viele Polymer/Lösungsmittel-Systeme nicht anwendbar ist. Trägt man jedoch [η]2/3/M1/3 Für verschiedene Lösungsmittel gegen M/[η] auf, so ergibt sich ein gemeinsamer Schnittpunkt, der aber nicht auf der Ordinate liegt. Die ungestörten Dimensionen, die mit Hilfe dieses Punktes berechnet werden, stimmen mit denjenigen überein, die nach andern Methoden erhalten wurden.

The relationship for the determination of unperturbed dimensions, as proposed by FLORY-FOX-SCHAEFGEN, has been found to be inapplicable to many polymer-solvent systems. However, while plotting [η]2/3/M1/3 vs. M/[η] for different solvents a common point of intersection which is not on the ordinate has been observed in all the cases. Unperturbed dimensions obtained from this point have been found to agree with those derived by using other methods.

6 Ill.

Electronic Resource

0025-116X

Chemistry ; Polymer and Materials Science

Wiley InterScience Backfile Collection 1832-2000

Chemistry and Pharmacology

Physics

Es wurde eine kritische Analyse der Daten durchgeführt, die von NAGASAWA und Mitarbeitern für den durch Lichtstreuungsmessungen am Natriumsalz der Polystyrolsulfonsäure (NaPSS) bestimmten zweiten Virialkoeffizienten erhalten wurden, und zwar an Hand der verschiedenen einschlägigen Theorien. Schließlich folgt eine kritische Erörterung über die vorgesehlagene Theorie zur getrennten Bestimmung der elektrostatischen Wechselwirkungen und des Kettencharakters des Polyelektrolyten, mit deren Hilfe die Abweichung vom idealen Zustand gemessen wurde.

A critical analysis of the data, obtained by NAGASAWA et al. for the second virial coefficient (A2) determined by light scattering measurements have been carried out for sodium polystyrene sulfonate (NaPSS). The result are analyzed in light of the different theories for the second virial coefficient. Finally, a critical discussion has been made of the theory proposed for the separate determination of the electrostatic interactions and the chain character of the polyelectrolyte by means of which the deviation from ideality are meansured.

6 Ill.

Electronic Resource