Search Results - (Author, Cooperation:J. P. Vasilakos)

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  1. 1
    Staff View
    Publication Date:
    2011-04-29
    Publisher:
    Nature Publishing Group (NPG)
    Print ISSN:
    0028-0836
    Electronic ISSN:
    1476-4687
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Animals ; Antigens, CD45/deficiency/metabolism ; Cell Wall/chemistry/immunology ; Cells, Cultured ; Humans ; Immunity, Innate/*immunology ; Immunological Synapses/*immunology ; Lectins, C-Type ; Macrophages/immunology ; Membrane Proteins/deficiency/genetics/*immunology ; Mice ; *Models, Immunological ; Nerve Tissue Proteins/deficiency/genetics/*immunology ; Phagocytosis/*immunology ; Reactive Oxygen Species/metabolism ; Receptor-Like Protein Tyrosine Phosphatases, Class 3/deficiency/metabolism ; Saccharomyces cerevisiae/chemistry/immunology ; Signal Transduction/immunology ; Solubility ; beta-Glucans/chemistry/immunology
    Published by:
    http://www.nature.com/

    Latest Papers from Table of Contents or Articles in Press
  2. 2
    Staff View
    ISSN:
    1573-675X
    Keywords:
    Caspase ; fluoromethylketones ; CPP32 ; PARP ; T cells
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Medicine
    Notes:
    Abstract CTLL cells undergo apoptosis when cultured in the absence of IL-2. The IL-1β-converting-enzyme (ICE)/ caspase family has been implicated as an integral component of some forms of apoptosis. Numerous members of the caspase family have been identified, and it appears as if caspase-3/CPP32 plays a critical role. Previously we demonstrated that ICE/caspase-1 expression increases in CTLL cells during apoptosis; however, inhibition of ICE activity did not abrogate apoptotic death. The purpose of this report is to determine if other members of the caspase family are involved in T cell apoptosis induced by growth factor starvation. We show that cytosolic CPP32-like activity, as measured by the cleavage of DEVD-pNA and poly(ADP-ribose) polymerase (PARP), increases during apoptosis following growth factor deprivation. Cytosolic CPP32-like activity is inhibited in cells treated with the broad spectrum ICE family inhibitor boc-aspartyl(OMe)-fluoromethylketone (D-FMK) and by VAD-FMK and DEVD-FMK which have greater specificity for CPP32-like ICE homologs; however, only the broad spectrum ICE inhibitor D-FMK inhibited apoptosis. Our results suggest that apoptosis induced by growth factor deprivation involves the caspase family, but increased CPP32-like activity is not sufficient to mediate apoptosis induced by IL-2 starvation.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1023/A:1026489104188

    Articles: DFG German National Licenses