Search Results - (Author, Cooperation:J. M. Silva)

2015-08-13

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Antioxidants/metabolism ; Carcinoma, Hepatocellular/genetics/*metabolism/*virology ; Carrier Proteins/genetics/*metabolism ; *Cell Culture Techniques ; Cell Line, Tumor ; Cells, Cultured ; Gene Library ; Genome, Viral/genetics ; *Genotype ; Hepacivirus/*genetics/*growth & development/physiology ; Host-Derived Cellular Factors/genetics/*metabolism ; Humans ; Lentivirus/genetics ; Lipid Peroxidation ; Lipoproteins/genetics/*metabolism ; Mutation/genetics ; RNA, Viral/biosynthesis/genetics ; Replicon/genetics ; Serum/virology ; Trans-Activators/genetics/*metabolism ; Transduction, Genetic ; *Virus Replication/genetics ; Vitamin E/metabolism

1365-4632

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Electronic Resource

2018-04-17

American Physical Society (APS)

1098-0121

1095-3795

Physics

Magnetism

2018-03-09

American Association for the Advancement of Science (AAAS)

0036-8075

1095-9203

Biology

Chemistry and Pharmacology

Geosciences

Computer Science

Medicine

Natural Sciences in General

Physics

Anthropology

2018-07-05

American Association for the Advancement of Science (AAAS)

2375-2548

Natural Sciences in General

1431-4630

Key words Enzymes ; Enology ; Enzyme preparations ; Pectolytic activity

Springer Online Journal Archives 1860-2000

Process Engineering, Biotechnology, Nutrition Technology

Abstract  The purpose of this research was to determine the activity of several pectolytic enzymes, such as pectin methyl esterase, endopolymethylgalacturonase, endopolygalacturonase, exopolymethylgalacturonase, endopolygalacturonase, pectin transeliminase and pectic acid transeliminase, from within preparations that are available on the Portuguese market. The selected preparations were as follows: 15 pectolytic enzyme preparations, one hemicellulolytic preparation and one glucanase preparation. The measurements of enzyme activity were carried out using model solutions with 50 mg·l–1 SO2, pH 3.2, 25°C, with and without 12% ethanol (v/v), and a 24-h incubation period. Quantitatively, the results proved to be heterogeneous, significantly varying from preparation to preparation: all pectolytic enzyme activities in the 17 preparations analysed in this study were significantly different. The presence of ethanol reduced the enzymatic activity, apart from that of endopolymethylgalacturonase, and, in general, an increase of enzyme concentration raised the pectolytic enzyme activity.

Electronic Resource

1569-8041

circulating DNA ; plasma DNA alterations ; prognostic factor ; SCLC ; tumor DNA alterations

Springer Online Journal Archives 1860-2000

Medicine

Abstract Background:Small-cell lung cancer (SCLC), one of the major types of lung cancer, is associated with many different somatic molecular genetic changes. These alterations, observed in tumor DNA, have also been identified in the plasma DNA of patients. We undertook the present study to make a prospective investigation into the correlation between abnormal plasma DNA and patient survival. Patients and methods:Thirty-five patients with SCLC were selected after histological diagnosis. Polymorphic markers (ACTBP2, UT762 and AR) were chosen for their reported high rate of alterations in SCLC and analyzed in tumor tissue, normal blood cells and plasma DNA. Furthermore, we looked for mutations of the TP53 gene in tumor and plasma DNA. Results:In 25 patients (71%) at least one molecular change precisely matching that of the primary tumor was detected in the plasma DNA. No difference in survival was observed between patients with aberrant plasma DNA and patients without plasma DNA alterations. However, patients with microsatellite modifications and TP53 mutations concomitantly, showed a significant difference (P = 0.02) in survival compared with patients bearing only one of these molecular changes. In 15 cases it was possible to find a correlation either between tumor response and disappearance of abnormal plasma DNA, or tumor progression and persistence of plasma DNA alterations. Conclusions:Free plasma DNA with molecular alterations is present to a high degree in plasma DNA of SCLC patients and may have a role as a prognostic factor.

Electronic Resource

1572-9613

Renormalization group ; Glauber dynamics ; Ising chain ; critical dynamics ; anomalous dynamics ; breakdown of dynamic scaling ; dynamic correlation length

Springer Online Journal Archives 1860-2000

Physics

Abstract We analyze a 1D Ising system with anomalous distributions of nearest neighbor interactions and show that the single-spin-flip dynamics exhibit breakdown of dynamic scaling. The results are obtained by a real-space numerical method applied to the exact equations of motion and they may be explained by domain wall motion arguments reformulated in terms of extreme value statistics.

Electronic Resource

1588-2837

Springer Online Journal Archives 1860-2000

Chemistry and Pharmacology

Abstract The treatment of MFI zeolites with ammonium hexafluorosilicate causes a selective dealumination of their outer surface. This dealumination improves the selectivity to the para isomer of m-xylene isomerization.

Electronic Resource

1741-2765

Springer Online Journal Archives 1860-2000

Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics

Abstract The use of modal-analysis techniques as nondestructive methods for the detection of cracks in beams is a current field of interest for various researchers. However, an extensive search of the published literature shows that there is very little information on experimental results, thus making it almost impossible for theoretical researchers to use published experimental data to validate their models. The authors, who are currently working in the field of crack detection through modal analysis, performed an extensive experimental dynamic analysis of free-free beams with the objective of validating theoretical techniques, under development, for the prediction of the location and depth of cracks in straight beams. This paper describes the first part of the authors' work. The experimental techniques used are described and results obtained for various locations and depths of cracks in a straight free-free beam are presented.

Electronic Resource

0197-8462

60-Hz ; field meter ; exposure assessment ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology

Wiley InterScience Backfile Collection 1832-2000

Biology

Physics

A small, lightweight meter has been developed for magnetic-field measurements, particularly those needed for exposure-assessment purposes. This meter, known as the AMEX-3D, continuously measures all three axes of magnetic-flux density and electronically combines the data into a single estimate of cumulative exposure to the root-mean-square (rms) resultant flux density. The AMEX-3D weighs about 120 g, measures 2.7 cm x 5.1 cm x 10.2 cm, and is battery powered. Two panel-mounted jacks are provided for measuring battery voltage and for reading cumulative exposure data from the unit. The instrument has, within 3 dB, a flat response to magnetic flux densities at all frequencies in its 30-1,000 Hz bandwidth. A detailed analysis of error sources in the AMEX-3D leads to an estimate of ±20% as the accuracy of the instrument over its dynamic range, which extends from 0.02 to 15 μT. The AMEX-3D was tested in the field by asking electricutility distribution linemen to wear AMEX-3D and EMDEX meters simultaneously while working. Agreement between the two measures of exposure was excellent. 1992 Wiley-Liss, Inc.

7 Ill.

Electronic Resource