Search Results - (Author, Cooperation:J. Lukes)

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  1. 1
    Staff View
    Publication Date:
    2015-05-23
    Publisher:
    American Association for the Advancement of Science (AAAS)
    Print ISSN:
    0036-8075
    Electronic ISSN:
    1095-9203
    Topics:
    Biology
    Chemistry and Pharmacology
    Computer Science
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Animals ; *Biodiversity ; DNA Barcoding, Taxonomic ; DNA, Ribosomal/genetics ; Eukaryota/*classification/genetics ; Oceans and Seas ; Phylogeny ; Plankton/*classification/genetics ; Ribosomes/genetics ; Sequence Analysis, DNA ; Sunlight
    Published by:
    http://www.aaas.org/

    Latest Papers from Table of Contents or Articles in Press
  2. 2
    VONDRUSKOVÁ, E. ; BURG, J. ; ZÍKOVÁ, A. ; ERNST, N.L. ; STUART, K. ; BENNE, R. ; LUKES, J.

    Oxford, UK : Blackwell Science Inc
    Published 2005
    Staff View
    ISSN:
    1550-7408
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Biology
    Notes:
    In trypanosomes, MRP1 and MRP2 (previously called gBPx and gBPy, in which x and y indicate the MW of these proteins in a particular species) are guide (g)RNA binding proteins that are part of a large heteromeric complex that may play a role in U-insertion/deletion editing of mitochondrial mRNAs. In order to shed more light on the function of these proteins, we generated procyclic Trypanosoma brucei cell lines in which the levels of MRP 1 and/or MRP2 mRNA were downregulated by RNA interference (RNAi). Here we report that the RNAi-mediated knockdown of MRP1 and/or MRP2 resulted in severe growth inhibition and loss of both proteins. This loss occurred even in cells in which only one of the MRPs was targeted by RNAi, indicating a mutual dependence for stability of these proteins. The elimination of the MRPs substantially reduced the levels of edited cytB and RPS12 mRNAs, but resulted in little or no reduction of edited cox2, cox3 and A6 mRNAs, as measured in poisoned primer extension analyses. Surprisingly, we found a five-fold increase in ND7 mRNA editing in MRP1+2 double knockdown cells. In addition, the knockdowns also resulted in reduction in the amounts of mRNAs that do not undergo RNA editing (cox1, ND4 and ND5 mRNAs), but little change was observed for mitoribosomal 12S rRNA. Together, the results indicate that in procyclic T. brucei, MRP1 and MRP2 play a role in transcript-specific editing and other RNA processing activities.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1111/j.1550-7408.2005.05202003_5_6.x

    Articles: DFG German National Licenses
  3. 3
    Lukes, J. ; Vavra, J.V.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0022-2011
    Keywords:
    Aedes, mosquito ; Microsporidium aedium n. sp ; gastric caeca ; microsporidia
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1016/0022-2011(91)90072-X

    Articles: DFG German National Licenses
  4. 4
    Wagner, A. ; Fortier, N. ; Giroux, A. ; Lukes, J. ; Snyder, L. M.
    Springer
    Published 1979
    Staff View
    ISSN:
    1420-9071
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Medicine
    Notes:
    Summary Patients with iron deficiency whether uncomplicated or associated with other types of anemias, had plasma catecholamine levels which were significantly increased above normal controls. Patients with a variety of other anemias had no significant increase in catecholamine levels. Plasma catecholamine levels in uncomplicated iron deficient patients approached normal values as early as 3 h following oral FeSO4.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/BF01960396

    Articles: DFG German National Licenses