Search Results - (Author, Cooperation:J. Linehan)

2015-09-17

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

2015-09-12

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Adult ; Alleles ; Alzheimer Disease/*etiology/genetics/metabolism/pathology ; Amyloid beta-Peptides/administration & dosage/analysis/*metabolism ; Autopsy ; Blood Vessels/metabolism/pathology ; Case-Control Studies ; Cerebral Amyloid Angiopathy/*etiology/metabolism/pathology ; Creutzfeldt-Jakob Syndrome/complications/*etiology/metabolism ; *Drug Contamination ; Endothelium, Vascular/metabolism/pathology ; Gray Matter/metabolism/pathology ; Human Growth Hormone/*administration & dosage ; Humans ; *Iatrogenic Disease ; Middle Aged ; Prions/administration & dosage/metabolism ; Risk Factors

1749-6632

Blackwell Publishing Journal Backfiles 1879-2005

Natural Sciences in General

Electronic Resource

1573-9686

Springer Online Journal Archives 1860-2000

Medicine

Technology

Electronic Resource

1573-9686

Oxidation-reduction ; Toluidine blue O ; Endothelium ; Cell: column chromatography ; Membrane ; Electron transport ; Pulmonary ; Endothelial cell ; Cell culture

Springer Online Journal Archives 1860-2000

Medicine

Technology

Abstract Thiazine dyes such as toluidine blue O (TBO) are reduced at the luminal endothelial surface. The purpose of this study was to determine the rate of this reaction in endothelial cells in culture. A multiple indicator dilution method was used to measure the reaction kinetics during transient passage of a TBO-containing bolus through a chromatographic column filled with bovine pulmonary arterial endothelial cells grown on microcarrier beads (cell-column). A bolus containing TBO and an inert extracellular reference indicator (FITC-Dextran) was injected upstream of the cell-column, and the indicator concentrations were measured downstream using on-line photodetection. The effects of column flow rate, PO2, and TBO concentration were studied. The fraction of TBO reduced upon passage through the cell-column decreased with increasing flow indicating that the reaction rate rather than TBO delivery controlled TBO reduction. The fraction of TBO reduced did not change with PO2 or dose in the ranges studied. TBO reduction was about 10 times that for steady state TBO sequestration by these cells which, along with the lack of a PO2 effect indicates that the rapid rate of reduction is not the rate-limiting step in steady state sequestration. PAC98: 8722Fy, 8220-w

Electronic Resource

1573-9686

Flow distribution ; Axial diffusion ; Mathematical model ; Perfusion heterogeneity ; Relative dispersion ; Heterogeneity ; Capillary transit times ; Indicator dilution

Springer Online Journal Archives 1860-2000

Medicine

Technology

Abstract To mathematically model multiple indicator dilution (MID) data for the purpose of estimating parameters descriptive of indicator-tissue interactions, it is necessary to account for the effects of the distribution of capillary transit times, hc(t) In this paper, we present an efficient approach for incorporating hc(t) in the mathematical modeling of MID data. In this method, the solution of the model partial differential equations obtained at different locations along the model capillary having the longest transit time provides the outflow concentrations for all capillaries. When weighted by hc(t) these capillary outflow concentrations provide the outflow concentration versus time curve for the capillary bed. The method is appropriate whether the available data on capillary dispersion are in terms of capillary transit time or relative flow distributions, and whether the dispersion results from convection time differences among heterogeneous parallel pathways or axial diffusion along individual pathways. Finally, we show that the knowledge of a relationship among the moments of hc(t) rather than hc(t) per se, is sufficient information to account for the effect of hc(t) in the mathematical modeling interpretation of MID data. This relationship can be determined by including a flow-limited indicator in the injected bolus, thus providing an efficient means for obtaining the experimental data sufficient to account for capillary flow and transit time heterogeneity in MID modeling. © 1998 Biomedical Engineering Society. PAC98: 8745Ft, 8710+e, 0230Jr

Electronic Resource

0001-1541

Chemistry ; Chemical Engineering

Wiley InterScience Backfile Collection 1832-2000

Chemistry and Pharmacology

Process Engineering, Biotechnology, Nutrition Technology

An experimental investigation of an impinging water jet freezing on a melting solid surface has been carried out. Attention was focused on the stagnation region of an axisymmetric jet. In the experiment, a water jet was directed upward against the lower end of a meltable rod, having a diameter about twice that of the jet orifice. Solid octane (m.p. - 56.5°C) and solid mercury (m.p. - 38.9°C) served as the meltable materials. A laminar-axisymmetric flow model was developed to describe melting heat transfer in the presence of jet solidification within the impingement region. Measurements of the melting rate and conditions for the onset of jet solidification were found to agree quite well with the values predicted with this model.

8 Ill.

Electronic Resource