Search Results - (Author, Cooperation:J. Hume)
-
1Latest Papers from Table of Contents or Articles in PressD. P. Locke ; L. W. Hillier ; W. C. Warren ; K. C. Worley ; L. V. Nazareth ; D. M. Muzny ; S. P. Yang ; Z. Wang ; A. T. Chinwalla ; P. Minx ; M. Mitreva ; L. Cook ; K. D. Delehaunty ; C. Fronick ; H. Schmidt ; L. A. Fulton ; R. S. Fulton ; J. O. Nelson ; V. Magrini ; C. Pohl ; T. A. Graves ; C. Markovic ; A. Cree ; H. H. Dinh ; J. Hume ; C. L. Kovar ; G. R. Fowler ; G. Lunter ; S. Meader ; A. Heger ; C. P. Ponting ; T. Marques-Bonet ; C. Alkan ; L. Chen ; Z. Cheng ; J. M. Kidd ; E. E. Eichler ; S. White ; S. Searle ; A. J. Vilella ; Y. Chen ; P. Flicek ; J. Ma ; B. Raney ; B. Suh ; R. Burhans ; J. Herrero ; D. Haussler ; R. Faria ; O. Fernando ; F. Darre ; D. Farre ; E. Gazave ; M. Oliva ; A. Navarro ; R. Roberto ; O. Capozzi ; N. Archidiacono ; G. Della Valle ; S. Purgato ; M. Rocchi ; M. K. Konkel ; J. A. Walker ; B. Ullmer ; M. A. Batzer ; A. F. Smit ; R. Hubley ; C. Casola ; D. R. Schrider ; M. W. Hahn ; V. Quesada ; X. S. Puente ; G. R. Ordonez ; C. Lopez-Otin ; T. Vinar ; B. Brejova ; A. Ratan ; R. S. Harris ; W. Miller ; C. Kosiol ; H. A. Lawson ; V. Taliwal ; A. L. Martins ; A. Siepel ; A. Roychoudhury ; X. Ma ; J. Degenhardt ; C. D. Bustamante ; R. N. Gutenkunst ; T. Mailund ; J. Y. Dutheil ; A. Hobolth ; M. H. Schierup ; O. A. Ryder ; Y. Yoshinaga ; P. J. de Jong ; G. M. Weinstock ; J. Rogers ; E. R. Mardis ; R. A. Gibbs ; R. K. Wilson
Nature Publishing Group (NPG)
Published 2011Staff ViewPublication Date: 2011-01-29Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Animals ; Centromere/genetics ; Cerebrosides/metabolism ; Chromosomes ; Evolution, Molecular ; Female ; Gene Rearrangement/genetics ; Genetic Speciation ; *Genetic Variation ; Genetics, Population ; Genome/*genetics ; Humans ; Male ; Phylogeny ; Pongo abelii/*genetics ; Pongo pygmaeus/*genetics ; Population Density ; Population Dynamics ; Species SpecificityPublished by: -
2Articles: DFG German National LicensesAdams, J. Hume ; Doyle, D. ; Ford, I. ; Gennarelli, T. A. ; Graham, D. I. ; Mclellan, D. R.
Oxford, UK : Blackwell Publishing Ltd
Published 1989Staff ViewISSN: 1365-2559Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Diffuse axonal injury is one of the most important types of brain damage that can occur as a result of non-missile head injury, and it may be very difficult to diagnose post mortem unless the pathologist knows precisely what he is looking for. Increasing experience with fatal non-missile head injury in man has allowed the identification of three grades of diffuse axonal injury. In grade 1 there is histological evidence of axonal injury in the white matter of the cerebral hemispheres, the corpus callosum, the brain stem and, less commonly, the cerebellum; in grade 2 there is also a focal lesion in the corpus callosum; and in grade 3 there is in addition a focal lesion in the dorsolateral quadrant or quadrants of the rostral brain stem. The focal lesions can often only be identified microscopically. Diffuse axonal injury was identified in 122 of a series of 434 fatal non-missile head injuries–-10 grade 1, 29 grade 2 and 83 grade 3. In 24 of these cases the diagnosis could not have been made without microscopical examination, while in a further 31 microscopical examination was required to establish its severity.Type of Medium: Electronic ResourceURL: -
3Articles: DFG German National LicensesStaff View
ISSN: 1476-4687Source: Nature Archives 1869 - 2009Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsNotes: [Auszug] So far back as June, 1898, you published in NATURE short article from my pen dealing with “Horn-feeding Larvæ”; it opened up the question as to whether the larvæ of the insect Tinea vastella, Zell. = gigantella, Stn. = lucidella, WKr., fed on the horns of living animals. I mentioned at the time ...Type of Medium: Electronic ResourceURL: -
4Articles: DFG German National LicensesXIE, H. S. ; HSIAO, A. I. ; QUlCK, W. A. ; HUME, J. A.
Oxford, UK : Blackwell Publishing Ltd
Published 1996Staff ViewISSN: 1365-3180Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: The influence of water stress on the absorption and translocation of 14C-labelled fenoxapropethyl and imazamthabenz-methyl in Avena fatua L. (wild oat) was studied. The phytoioxicity to A. fatua of both herbicides with a droplet application was also examined under water stress conditions. The absorption of both fenoxaproethyl and imazamethabenz-methyl was reduced by waler stress when the plants were harvested within 24 h after herbicide application. Up to 48 h after the application, the translocation out of the treated lamina of both herbicides, based on percentage of applied 14C. was reduced under water stress conditions. When havested 96 h after herbicide application, however, water stress no longer significantly affeaed the absorption and translocation of either herbicide. When the herbicides were applied as individual droplets, water stress reduced the phytotoxicity of fenoxaprop-ethyl but not that of imazamethabenz-methyl. It is concluded that the changes in herbieide absorption and translocation may not be the major physiological processes associated with differential whole-plant response oi A faiua to fenoxaprop-ethyl and imazamefhabenz-methyl under water stress.Type of Medium: Electronic ResourceURL: -
5Articles: DFG German National LicensesStaff View
ISSN: 0156-7446Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionEconomicsURL: -
6Articles: DFG German National LicensesStaff View
ISSN: 0022-1325Topics: PsychologyURL: -
7Articles: DFG German National LicensesStaff View
ISSN: 1476-4687Source: Nature Archives 1869 - 2009Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsNotes: [Auszug] Weidmann2 first reported that the local anaesthetic cocaine depresses the maximum upstroke velocity (V^ax) of the action potential in sheep Purkinje fibres; his results also suggested that this effect was produced by a voltage-dependent shift in the inactivation mechanism governing /Na. Johnson and ...Type of Medium: Electronic ResourceURL: -
8Articles: DFG German National LicensesStaff View
ISSN: 1432-2013Keywords: Key words Chloride channels ; CFTR ; Protein kinase A ; Cardiac electrophysiologySource: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Abstract Cardiac Cl–channels may play an important role in both the physiological as well as the pathophysiological regulation of the heart. Due to the present controversy regarding the role that Na+ may play in the regulation of the adenosine 3′,5′-cyclic monophosphate (cAMP)-dependent Cl–channels in the heart, we have re-examined the effects of intracellular and extracellular Na+ on I Cl,cAMP in isolated guinea-pig ventricular myocytes. In conventional, whole-cell patch-clamp experiments, exchanging of extracellular Na+ for N-methyl-d-glucamine (NMG) did not have a significant effect on the magnitude of the I Cl,cAMP activated by external forskolin (FSK) in the presence of β-adrenergic and muscarinic receptor blockade compared with normal rundown of the current with time. Utilization of the amphotericin B-perforated-patch technique prevented rundown of FSK-stimulated I Cl,cAMP, and failed to reveal alterations in this conductance when NMG was substituted for extracellular Na+. Finally, intracellular dialysis of cells during whole-cell experiments with both 0 and 10 mM Na+-containing solutions failed to show any significant effect of intracellular Na+ on the magnitude of I Cl,cAMP. We therefore conclude that intracellular or extracellular Na+ plays little or no direct regulatory role in modulation of cAMP-dependent Cl–channels in heart.Type of Medium: Electronic ResourceURL: -
9Articles: DFG German National LicensesStaff View
ISSN: 1432-2013Keywords: Key words K+ channels ; pH ; Pulmonary artery ; Hypoxic pulmonary vasoconstrictionSource: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Abstract Voltage-dependent K+ currents (Kv) may play a role in hypoxic pulmonary vaso constriction. The effects of changes in extracellular pH (pHo) and intracellular pH (pHi) on Kv currents in smooth muscle cells isolated from canine pulmonary artery were studied using the amphotericin B perforated-patch technique for whole-cell recording. Under these conditions, cellular mechanisms for pHi regulation remain intact, and the effects of pHo were examined by directly changing the pH of external solutions and changes in pHi were produced by external application of weak extracellular acids and bases and the cation/H+ ionophore, nigericin. Ca2+-free external solutions were used to isolate whole-cell Kv currents from contaminating Ca2+-activated K+ currents. Extracellular acidification (pHo = 6.4–7.0) reduced Kv currents, produced a positive voltage shift in steady-state activation and reduced maximum Kv conductance (-g K). Extracellular alkalinization (pHo = 8.0–8.4) increased Kv currents, produced a small negative voltage shift in steady-state activation, and increased -g K. Intracellular acidification produced by exposure of cells to external sodium butyrate (20 mM) or nigericin (5 μg/ml) increased Kv currents, produced a negative voltage shift in steady-state activation, and increased -g K. Intracellular alkalinization produced by exposure of cells to external trimethylamine (20 mM) reduced Kv currents, produced a small positive voltage shift in steady-state activation and reduced -g K. These results suggest that the effects of pHo and pHi on Kv currents are distinctly different, but are consistent with reported effects of pHo and pHi on hypoxic pulmonary vasoconstriction, suggesting that such modulation may be mediated in part by pH-induced alterations in Kv channel activity.Type of Medium: Electronic ResourceURL: -
10Articles: DFG German National LicensesStaff View
ISSN: 1432-2013Keywords: Ionic channels ; Potassium channels ; Smooth muscle ; Patch-clamp ; VasodilatorsSource: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Abstract The effects of cromakalim (BRL 34915) and its (−) optical isomer, lemakalim (BRL 38227) on the activity of 265-pS Ca2+-activated K+ channels (BK channels) were examined in cell-attached and inside-out patches from canine colonic myocytes. In cell-attached patches lemakalim increased the open probability (P o) of BK channels. Mean NP o, where N is the number of channels per patch, at + 50 mV increased from 0.08 to 0.26 (20 μM lemakalim). In inside-out patches, cromakalim and lemakalim increased channel NP o rapidly and reversibly. This increase in NP o was due to a shift in half-maximal activation. Glyburide (20 μM) prevented the increase in NP o caused by lemakalim in cell-attached patches and reversed the increase in NP o in inside-out patches. Under conditions where Ca2+-activated K+ channels were maximally activated, lemakalim failed to increase current or induce a second type of K+ channel activity. When tetraethylammonium (200 μM) was added to the pipette solution to block the BK channel half maximally, lemakalim also failed to induce a second type of channel. Adenosine triphosphate (1 or 2 mM) applied to the inner surface of inside-out patches had no effect on P o of BK channels. Finally, the effects of lemakalim on ensemble average currents, constructed from multiple openings of BK channels in cell-attached patches was found to successfully mimic the effects of the drug on whole-cell membrane currents. We conclude that cromakalim and lemakalim activate BK channels in canine colonic cells. Whether this action participates in the membrane hyperpolarization and the decrease in frequency and duration of slow waves produced by these compounds in intact colonic muscles remains to be investigated.Type of Medium: Electronic ResourceURL: -
11Articles: DFG German National LicensesLevesque, P. C. ; Clark, C. D. ; Zakarov, S. I. ; Rosenshtraukh, L. V. ; Hume, J. R.
Springer
Published 1993Staff ViewISSN: 1432-2013Keywords: Cardiac electrophysiology ; Ion channels ; Chloride channels ; Action potentials ; Patch clampSource: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Abstract Modulation of the ventricular action potential by β-adrenergic activation of Ca2+, K+ and cyclic adenosine monophosphate (cAMP)-dependent Cl− channels was assessed in enzymatically isolated guinea-pig ventricular myocytes. The effectiveness and relative selectivity of 9-anthracene carboxylic acid (9-AC), as an antagonist of cAMP-dependent Cl− channels was also tested. Membrane currents and action potentials were recorded using the conventional whole-cell variant of the patch-clamp technique or with the amphotericin B perforated-patch technique. The β-adrenergic agonist isoproterenol either increased or decreased action potential duration depending on whether the dominant effect was on inward Ca2+ currents or on outward K+ or Cl− currents. When Ca2+ and K+ channel modulation was prevented by nisoldipine and low temperature respectively, β-adrenergic activation of Cl− channels caused a significant reduction in action potential duration and a slight depolarization of the membrane potential. The β-adrenergic-mediated effects were reversed by the Cl− channel blocker, 9-AC. In the absence of β-adrenergic stimulation, 9-AC had no detectable effects on action potentials or Ca2+ currents. These results suggest that β-adrenergic activation of Cl− channels is a potent mechanism for regulation of action potential duration and that 9-AC may be a useful, relatively specific, pharmacological tool for evaluating the physiological role of cAMP-activated Cl− channels in heart. 9-AC also reversed the ability of isoproterenol to antagonize prolongation of action potential duration by the class III antiarrhythmic agent E-4031.Type of Medium: Electronic ResourceURL: -
12Articles: DFG German National LicensesStaff View
ISSN: 1573-4919Keywords: electrophysiology ; patch clamp ; ionic channelsSource: Springer Online Journal Archives 1860-2000Topics: BiologyChemistry and PharmacologyMedicineNotes: Summary A variant of the whole-cell patch clamp technique is described which allows measurement of whole-cell ionic currents in small cells while minimizing cell dialysis with the pipette solution. The technique involves the application of negative pressure to the inside of small (〈 1 µm) tip diameter pipettes placed on the cell surface to achieve high resistance seals and membrane rupture. The technique has been used successfully in a variety of different types of cells to study membrane currents carried by Ca and K, currents generated by exchange carriers as well as electrical coupling between cells. Overall, the technique seems well suited for the study of ionic currents in small cells, and provides an alternative to conventional patch clamping techniques which necessitate intracellular dialysis.Type of Medium: Electronic ResourceURL: -
13Articles: DFG German National LicensesZschacke, F. H. ; Ray, F. E. ; Hume, J. ; Frerichs, G. ; Lochte, H. L. ; Hoover, Anna ; Whitnah, C. H. ; Jackson, J. ; Dietz, A. ; Guttmann, E. ; Dunbar, R. E. ; Freye, H. A. ; Wirth, C. ; Sperry, W. A. ; Hasselblatt, M. ; Burschkies, K. ; Gollnow, G. ; How, A. ; Trültzsch ; Mayer, F. ; Thiessen, G. W. ; Wertz, J. E. ; Szeszich, L. v. ; Vollbrecht, H. ; Hilpert, S.
Springer
Published 1934Staff ViewISSN: 1618-2650Source: Springer Online Journal Archives 1860-2000Topics: Chemistry and PharmacologyType of Medium: Electronic ResourceURL: -
14Articles: DFG German National LicensesStaff View
ISSN: 1432-136XSource: Springer Online Journal Archives 1860-2000Topics: BiologyMedicineNotes: Summary Nitrogen metabolism and urea kinetics were studied in rock hyraxes (Procavia habessinica) fed diets of different protein content. The maintenance nitrogen (N) requirement of the rock hyrax (311 mg·kg−0.75·24 h−1 of dietary N, or 209 mg·kg−0.75·24 h−1 of truly digestible N) is similar to that of several marsupial species, and thus lower than that of other eutherians. Urea recycled to the gut, measured with single injections of14C-urea, was 63% and 60% of urea entry rate on diets with 14.6% and 8.2% crude protein, respectively. Urea recycling increased to 70% when water intake was restricted, but decreased to 40% on a low (5.3%) protein diet, presumably because of a low energy intake. Urea utilization in the gut, measured with single injections of15N-urea, was 59% and 53% of urea degradation on the 14.6% and 8.2% protein diets, respectively. Urea utilization increased to 71% on the low protein diet, and increased to 98% with water restriction. The low maintenance nitrogen requirement appears to be the main physiological attribute of the rock hyrax enabling it to survive periods of low dietary protein availability. However, this low requirement can be related to the low basal metabolic rate of the Hyracoidea in general, and thus is not necessarily a primary adaptation to the environment.Type of Medium: Electronic ResourceURL: -
15Articles: DFG German National LicensesStaff View
ISSN: 0308-0501Keywords: Chemistry ; Polymer and Materials ScienceSource: Wiley InterScience Backfile Collection 1832-2000Topics: Architecture, Civil Engineering, SurveyingMechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision MechanicsNotes: Quantitative pyrolysis gas chromatography studies have been conducted on three grades of cross-linked polyester resin formulated for improved resistance to surface spread of flame. Pyrolysis temperatures from 723 to 1273 K were used, and these values correspond to ‘surface’ temperatures of materials exposed to heat fluxed of 15-149 kW m-2 which are typical of conditions experienced in real fires. Comparison is made with earlier macro studies on the evolution of smoke from plastics materials exposed to similar heat fluxes under an inert atmosphere. There is some correlation between the smoke obscuration data obtained in previous macro studies and the yield of volatile aromatic products evolved on pyrolysis. The presence of flame retardants increases the formation of carbon in the pyrolysis residue and decreases the yield of volatile aromatic products isolated. The results are considered in the context of mechanisms of smoke formation.Additional Material: 6 Tab.Type of Medium: Electronic ResourceURL: