Search Results - (Author, Cooperation:J. H. Sinclair)
-
1Latest Papers from Table of Contents or Articles in PressM. P. Weekes ; S. Y. Tan ; E. Poole ; S. Talbot ; R. Antrobus ; D. L. Smith ; C. Montag ; S. P. Gygi ; J. H. Sinclair ; P. J. Lehner
American Association for the Advancement of Science (AAAS)
Published 2013Staff ViewPublication Date: 2013-04-13Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyComputer ScienceMedicineNatural Sciences in GeneralPhysicsKeywords: Antigens, CD34/analysis ; Cell Line, Tumor ; Cytomegalovirus/genetics/*physiology ; Cytomegalovirus Infections/*metabolism/*virology ; Dendritic Cells/physiology ; Down-Regulation ; Humans ; Lysosomes/metabolism ; Monocyte-Macrophage Precursor Cells/metabolism/virology ; Monocytes/metabolism/virology ; Multidrug Resistance-Associated Proteins/genetics/*metabolism ; Vincristine/metabolism/pharmacology ; Viral Proteins/*metabolism ; *Virus LatencyPublished by: -
2Articles: DFG German National LicensesSinclair, J. H. ; Sang, J. H. ; Burke, J. F. ; Ish-Horowicz, D.
[s.l.] : Nature Publishing Group
Published 1983Staff ViewISSN: 1476-4687Source: Nature Archives 1869 - 2009Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsNotes: [Auszug] Copia is present at 20-40 copies per haploid genome in flies, and at about 150 copies per cell in Drosophila tissue culture cells3,4. It is also found as extrachromosomal circular molecules the structure of which resembles that of an unintegrated circular retroviral provirus2. The integrated copia ...Type of Medium: Electronic ResourceURL: -
3Articles: DFG German National LicensesBurke, J. F. ; Pinchin, S. M. ; Ish-Horowicz, D. ; Sinclair, J. H. ; Sang, J. H.
Springer
Published 1984Staff ViewISSN: 1572-9931Source: Springer Online Journal Archives 1860-2000Topics: BiologyMedicineNotes: Abstract We have used DNA-mediated gene transfer to introduce intoDrosophila melanogaster cells DNA sequences for which no selective criteria exist. We have introduced a Drosophila heat-shock locus into culturedDrosophila cells by calcium phosphate cotransfection with the copia vector pCV31gpt and selection for xanthine utilization. We recovered cell lines containing between three and about 50 copies of both pCV31 gpt and pMH10A, a cloned 87A7hsp70 heat-shock locus that encodes a mutant 40,000-dalton heat-shock protein (hsp40). The stable inheritance of the transformed DNAs argues that the input DNAs have integrated into the genome. We show that this is indeed the case for one cell line by cloning back the transfected DNA and detecting the flanking chromocentral sequences by in situ hybridization. Surprisingly, the integrated hsp70 genes are not expressed. This report represents the first example of the cointroduction of DNA sequences intoDrosophila cells by cotransfection with a dominant selectable marker.Type of Medium: Electronic ResourceURL: -
4Articles: DFG German National LicensesStaff View
ISSN: 1572-9931Source: Springer Online Journal Archives 1860-2000Topics: BiologyMedicineNotes: Abstract At the nonpermissive temperature, the shibirets gene of Drosophilacauses adult paralysis and cell death during development. We have used DNA-mediated gene transfer with wildtype genomic DNA to rescue the lethal phenotype of shits cultured cells and have isolated cell lines which grow at the nonpermissive temperature of 29° C. By using restriction endonucleases, we confirm that this rescue is DNA sequence dependent. Further, cells cotransfected with wildtype genomic DNA and plasmid DNA contain plasmid sequences when selected for survival at 29° C. This confirms the uptake of DNA by the transformed cells and provides a simple temperature selection technique allowing coselection of genes which cannot themselves be selected for.Type of Medium: Electronic ResourceURL: -
5Articles: DFG German National LicensesStaff View
ISSN: 1741-2765Source: Springer Online Journal Archives 1860-2000Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision MechanicsNotes: Abstract A combined theoretical and experimental investigation was conducted to assess the suitability of the 10-deg off-axis tensile-test specimen for the intralaminar-shear characterization of unidirectional composites. Composite mechanics, a combined-stress failure criterion and a finiteelement analysis were used to determine theoretically the stress-strain variation across the specimen width and the relative stress and strain magnitudes at the 10-deg plane. Strain gages were used to measure the strain variation across the specimen width at specimen midlength and near the end tabs. Specimens from Mod-I/epoxy. T-300/epoxy, and S-glass/epoxy were used in the experimental program. It was found that the 10-deg off-axis tensile-test specimen is suitable for intralaminar-shear characterization and it, is recommended that it should be considered as a possible standard test specimen for such a characterization.Type of Medium: Electronic ResourceURL: -
6Articles: DFG German National LicensesStaff View
ISSN: 0032-3888Keywords: Chemistry ; Chemical EngineeringSource: Wiley InterScience Backfile Collection 1832-2000Topics: Chemistry and PharmacologyMechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision MechanicsPhysicsNotes: Finite element analyses were performed to investigate theoretically the effects of in-plane and out-of-plane eccentricities, bending or twisting, and thickness nonuniformity on the axial stress and strain variations across the width of off-axis specimens. The results are compared with measured data and are also used to assess the effects of these eccentricities on the fracture stress of off-axis fiber composites. Guidelines for detecting and minimizing the presence of eccentricities are described.Additional Material: 14 Ill.Type of Medium: Electronic ResourceURL: -
7Articles: DFG German National LicensesStaff View
ISSN: 0272-8397Keywords: Chemistry ; Chemical EngineeringSource: Wiley InterScience Backfile Collection 1832-2000Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision MechanicsNotes: A mechanics theory is developed for predicting the physical thermal, hygral, and mechanical properties (including various strengths) of unidirectional intraply hybrid composites (UIHC) based on unidirectional properties of the constituent composites. Procedures are described which can use this theory in conjunction with composite mechanics computer codes and general purpose structural analysis finite element programs for the analysis/design of structural components made from intraply hybrid angleplied laminates (IHAL). Comparisons with limited data show that this theory predicts mechanical properties of UIHC and flexural stiffnesses of IHAL which are in good agreement with experimental data. The theory developed herein makes it possible to design and optimize structural components from IHAL based on a large class of available constituent fibers.Additional Material: 8 Ill.Type of Medium: Electronic ResourceURL: -
8Articles: DFG German National LicensesStaff View
ISSN: 0272-8397Keywords: Chemistry ; Chemical EngineeringSource: Wiley InterScience Backfile Collection 1832-2000Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision MechanicsNotes: Criteria have been developed for identifying, characterizing, and quantifying fracture modes in high-modulus graphite-fiber/resin unidirectional composites subjected to off-axis tensile loading. Procedures are described which use sensitivity analyses and off-axis data to determine the uniaxial strength of fiber composites. It was found that off-axis composites failed by three fracture modes, which produce unique fracture surface characteristics. The stress that dominates each fracture mode and the load angle range of its dominance can be identified. Linear composite mechanics is adequate to describe quantitatively the mechanical behavior of off-axis data are comparable to those measured in uneasily tests.Additional Material: 13 Ill.Type of Medium: Electronic ResourceURL: