Search Results - (Author, Cooperation:J. F. Jia)
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1C. L. Song ; Y. L. Wang ; P. Cheng ; Y. P. Jiang ; W. Li ; T. Zhang ; Z. Li ; K. He ; L. Wang ; J. F. Jia ; H. H. Hung ; C. Wu ; X. Ma ; X. Chen ; Q. K. Xue
American Association for the Advancement of Science (AAAS)
Published 2011Staff ViewPublication Date: 2011-06-18Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyComputer ScienceMedicineNatural Sciences in GeneralPhysicsPublished by: -
2M. X. Wang ; C. Liu ; J. P. Xu ; F. Yang ; L. Miao ; M. Y. Yao ; C. L. Gao ; C. Shen ; X. Ma ; X. Chen ; Z. A. Xu ; Y. Liu ; S. C. Zhang ; D. Qian ; J. F. Jia ; Q. K. Xue
American Association for the Advancement of Science (AAAS)
Published 2012Staff ViewPublication Date: 2012-03-17Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyComputer ScienceMedicineNatural Sciences in GeneralPhysicsPublished by: -
3Staff View
ISSN: 0039-6028Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: PhysicsType of Medium: Electronic ResourceURL: -
4Staff View
ISSN: 1432-203XKeywords: Key wordsAstragalus adsurgens ; Protoplast culture ; Plant regeneration ; EmbryogenesisSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract A reproducible release of viable protoplasts was obtained from friable calli of Astragalus adsurgens. Protoplasts underwent sustained divisions and formed cell colonies when cultured in either liquid or agarose-solidified Kao and Michayluk (1975) protoplast medium (KM8P) supplemented with 1.5 mg/l 2,4-D, 0.5 mg/l BA and 0.5 M glucose. Compared to liquid culture, agarose bead culture improved division frequency effectively, the two culture systems showing a plating efficiency of 0.8±0.5% and 6.5±0.7%, respectively. Upon transfer to Murashige and Skoog (1962) medium (MS) with 1–2 mg/l BA, alone or in combination with NAA or 2,4-D at 0.1 mg/l, the protoplast-derived calli produced complete plantlets through somatic embryogenesis. The maximum percentage of calli producing somatic embryos (52.5± 2.2%) occurred on MS medium containing 0.1 mg/l NAA and 1 mg/l BA, whereas the maximum number of calli regenerating plantlets (64.7±6.2) was obtained on MS medium with 0.1 mg/l NAA and 2 mg/l BA.Type of Medium: Electronic ResourceURL: -
5Staff View
ISSN: 1432-203XKeywords: Key words Callus induction ; Plant regeneration ; Astragalus adsurgensSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract An efficient procedure was developed for inducing callus and plant regeneration using hypocotyl segments of Astragalus adsurgens. The combinations and concentrations of different growth regulators were shown to be critical factors for both the frequency and the type of callus formation as well as for the potential of callus differentiation. Of the four morphologically distinct types of calli that were induced, a friable, yellow callus, i.e. type I, induced on MS medium supplemented with 9.0 µM 2,4-dichlorophenoxyacetic acid and 2.2 µM N6-benzylaminopurine (BA), and then transferred to MS medium containing 0.5 µM α-naphthaleneacetic acid and 8.9 µM BA, exhibited the maximum frequency of shoot regeneration (75%). After regenerated shoots were transferred onto half-strength MS medium without growth regulators, they rooted and complete plants were obtained. Plantlet regeneration from callus cultures required 7–8 weeks.Type of Medium: Electronic ResourceURL: