Search Results - (Author, Cooperation:J. Cha)

2018-04-07

American Chemical Society (ACS)

0002-7863

1520-5126

Chemistry and Pharmacology

2013-02-19

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

CLOCK Proteins/chemistry/*genetics/metabolism ; Circadian Clocks/genetics/physiology ; Circadian Rhythm/genetics/physiology ; Codon/*genetics ; Feedback, Physiological ; Fungal Proteins/*chemistry/genetics/*metabolism ; *Gene Expression Regulation, Fungal ; *Neurospora crassa/chemistry/genetics/metabolism ; Open Reading Frames ; Phosphorylation ; Protein Conformation ; Protein Stability ; Trypsin/metabolism

2018-01-18

The American Society for Microbiology (ASM)

0099-2240

1098-5336

Biology

1524-475X

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Experimentally induced wounds in animal models are useful in gaining a better understanding of the cellular and molecular processes of wound healing and in the initial evaluation of the safety and effectiveness of potential therapeutic agents. However, studying delayed healing has proven difficult in animals, whose wounds heal within a few days. In this report, we describe a novel method for establishing mouse wounds that require up to more than three weeks for complete closure, and we show the validity of this model in Smad3 null mice, which are known to display accelerated healing. Full-thickness wounds, measuring 0.3 by 1.0 cm, were made down to fascia on the dorsal aspect of the mouse tail in Smad3 KO mice and control littermates, approximately 1 cm distal to the body of the animal. The wounds were left to heal by secondary intention and were assessed histologically by computerized planimetry for wound closure at various times after wounding. These wounds in wild-type mice displayed delayed healing, with full closure occurring between 14 and 25 days after wounding. Complete closure of similar wounds in Smad3 null mice healed 30% faster (p 〈 0.01). By immunostaining with ki67, a marker for proliferation, Smad3 null animals also showed increased proliferation of dermal wound cells. Cultured dermal fibroblasts from Smad3 null mice showed increased baseline DNA synthesis and, interestingly, enhanced response to TGF-β1. By Western blot analysis, Smad3 null mice fibroblasts showed a compensatory increase in MAPK phosphorylation in response to TGF-β1, suggesting that MAPK overcompensation together with loss of Smad3 may be involved in the modulation of faster healing. We conclude that this novel tail wounding model can be useful for studying delayed or prolonged wound closure.Experimentally induced wounds in animal models can be useful in gaining a better understanding of the cellular and molecular processes of wound healing. Such models have also proven themselves valuable in the initial evaluation of the safety and effectiveness of potential therapeutic agents targeted for chronic non-healing wounds. (Gottrup, Agren et al. 2000). However, no ideal animal model exists which reliably reproduces delayed healing. In the mouse, a mammal whose genome has been completely cloned and which is easily manipulated genetically, wounds normally heal within a few days, and with a great deal of contraction. (Morris, Wu et al. 1997; Gottrup, Agren et al. 2000) There are models utilizing either genetically altered and inbred mice with certain characteristics that cause delayed healing. (Carmeliet 1995) However, it would be useful to have wound healing models that are applicable to most wild type mice used as controls and which would have a large enough window of observation before healing occurs. In this report, we describe a novel method for studying delayed healing in mice. This method utilizes full-thickness wounds made down to fascia on the dorsal and mostly hairless aspect of the mouse tail. The wounds are left to heal by secondary intention and assessed histologically by computerized planimetry for wound closure at various times after wounding. In this first report, the validity of the model was determined by studying control littermates and Smad3 null mice, which have been shown to display accelerated healing. The results shown here suggest that this is a useful model for studying delayed healing in mice.

Electronic Resource

1439-0329

Blackwell Publishing Journal Backfiles 1879-2005

Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

To determine the phylogenetic positions of two new species, Armillaria jezoensis and Armillaria singula, and one new subspecies, Armillaria mellea suhsp. nipponica, the nucleotide sequences of the intergenic spacers (IGS) of their ribosomal DNA were investigated, and compared with those of tour other Armillaria species from Japan, and those of nine Armillaria species from Europe and North America. We conclude that Armillaria jezoensis, and Armillaria singula belong to the Armillaria gallica cluster as Armillaria cepistipes, Armillaria gallica and Armillaria sinapina from Japan. Two isolates of Armillaria ostoyae from Japan were placed within the Armillaria ostoyae cluster. Armillaria mellea subsp. nipponica had an IGS sequence as long as the IGS of Armillaria mellea from Europe and North America. However, the IGS sequences of Armillaria mellea subsp. nipponica, whose basidium base lacks a clamp connection could not be satisfactorily aligned with the IGS sequences of other species possessing this morphological feature.

Electronic Resource

1365-2133

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Electronic Resource

0378-1119

(Recombinant DNA ; T/A cloning vectors ; phage M13mp)

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Electronic Resource

0378-1119

Recombinant DNA ; T/A cloning vectors ; phage M13mp

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Electronic Resource

0029-5493

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Energy, Environment Protection, Nuclear Power Engineering

Electronic Resource

0003-3472

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Electronic Resource

0040-4039

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Chemistry and Pharmacology

Electronic Resource

0040-4039

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Chemistry and Pharmacology

Electronic Resource

1572-8900

Starch ; loose-fill ; humidity ; aging

Springer Online Journal Archives 1860-2000

Chemistry and Pharmacology

Energy, Environment Protection, Nuclear Power Engineering

Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics

Abstract Loose-fill packaging sponges were extruded from mixtures of 54–62% hydroxypropylated (HP ≍ 5%) amylomaize V (50% amylose) and wheat starches, 17–24% synthetic polymer, ∼13% water, 7% blowing agent(s), and 0.5% nucleating agent. One product made from 28% HP wheat starch, 28% HP amylomaize V starch, 12% ethylene vinyl alcohol (EVOH) copolymer, 8% polystyrene (PS), and 3% polystyrene maleic anhydride (PSMA) copolymer, plus the other raw materials, had a compressibility and resilience that matched those of expanded polystyrene (EPS), although its bulk density was four times higher. The starchy sponge showed 16% shrinkage in volume at 90% relative humidity and was 2% soluble in excess water, both at 25°C. After aging for 18 months near 25°C, the HP starchy sponge gave only a trace of fines in a simulated shipping test, compared to 20% fines from a biodegradable, starch-based, loose-fill sponge of commerce.

Electronic Resource

0040-4039

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Chemistry and Pharmacology

Electronic Resource

0040-4039

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Chemistry and Pharmacology

Electronic Resource

0040-4039

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Chemistry and Pharmacology

Electronic Resource

1432-0509

Key words: Bile ducts—Bile ducts, CT—Bile ducts, neoplasm.

Springer Online Journal Archives 1860-2000

Medicine

Abstract Background: To assess the accuracy of spiral computed tomography (CT) in predicting the resectability of Klatskin tumor as determined by vascular invasion. Methods: Twenty-one consecutive patients with Klatskin tumor who had undergone laparotomy were included in this study. The preoperative thin-section (5-mm-thick) spiral CT scans of these patients were assessed for the surgical resectability of tumor by evaluating the vascular invasion. The criterion for vascular invasion indicating unresectability was the tumoral invasion of the proper hepatic artery or main portal vein or simultaneous invasion of one side of the hepatic artery and the other side of the portal vein. Results: All nine patients with tumors thought to be unresectable on the basis of CT findings had tumors that were unresectable at surgery (positive predictive value, 100%). Of 12 patients with tumors thought to be resectable, six had resectable tumors (negative predictive value, 50%). Spiral CT failed to detect small hepatic metastasis (n= 1), lymph node metastasis (n= 1), extensive tumor (n= 2) and variation of bile duct (n= 2), which precluded surgical resection. Conclusion: Spiral CT is a reliable method for detecting vascular invasion and unresectable tumors. However, it has limitations in detecting variations of the bile duct or the intraductal extent of tumor.

Electronic Resource

1432-1793

Springer Online Journal Archives 1860-2000

Biology

Abstract Eupolymnia nebulosa (Montagu) is a widely distributed terebellid polychaete that builds its tubes on the coastal shelf in areas with mixed soft and hard bottoms. From a long-term survey in the Bay of Banyuls, France (NW Mediterranean), we found an advancement of the timing of the spawning period coincident with a delayed breakdown of the thermocline. We postulate that persistent high temperatures can influence gamete development by stimulating oocyte growth, resulting in earlier spawning. During 1992 and 1993, we used a between-individuals experimental approach to assess the possible effect of temperature on oocyte growth based on: (1) determination of the growing fraction of the oocyte population (i.e. oocyte net growth); (2) identification of differences in oocyte growth-rate among females; (3) comparison of non-significantly different size-distributions of the growing oocyte fraction (net oocyte size-distributions) at the beginning of the experiments with those at the end. No effect of temperature on oocyte growth was detectable at the population level, but a positive individual response to prolonged high temperature was evident. Thus, the lack of a significant response by the population to prolonged high temperature does not imply a lack of individual response. We propose a model of oocyte-growth dynamics based on temperature that incorporates previous observations of extended oogenesis and oocyte growth during periods of both increasing and decreasing temperature and on the scattered pattern of oocyte size-distributions at the onset of spawning.

Electronic Resource

1573-5036

Springer Online Journal Archives 1860-2000

Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Summary Roots of detopped tobacco plants (Nicotiana tabacum var. Virginia Gold) were exposed to Na, K, and Ca salts or to water, and cation transfer to xylem vessels was measured. In some cases plants had been exposed to Na in addition to regular nutrient solutions before detopping. Calcium in the external medium greatly depressed the transport of Na from the external medium to the xylem vessels and it often stimulated the transfer of K from the external medium to the xylem vessels. The K/Na ratio in the exudate thus was dependent upon the Ca content of the external medium under these conditions. In contrast, externally applied Ca or Ca deficiency had very little effect on the transfer of preaccumulated K and Na from compartments within roots to the xylem vessels. The K/Na ratio in the exudate under these conditions was not related to Ca levels nor to mild Ca deficiency. The ratios decreased with time after detopping regardless of Ca level. Intact plants accumulated more Na than did root systems of detopped plants in a 6-day period.

Electronic Resource

1573-5036

Springer Online Journal Archives 1860-2000

Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Summary Chromium applied to a noncalcareous soil at 50 ppm did not decrease yields of bush beans (Phaseolus vulgaris L. var Improved Tendergreen), but when EDTA (ethylenediamine tetraacetic acid) was added with it, it did. Very little Cr was present in leaves. In solution culture 10-5 M Cr and higher were toxic. With solution culture the highest level of Cr in leaves was about 30 ppm and in general there was a decreasing gradient in Cr from roots to stems to leaves. EDTA had less effect in solution cultures on Cr toxicity because the Cr was already in solution. Chromium toxicity decreased cation levels in plants.

Electronic Resource