Search Results - (Author, Cooperation:J. C. Espinoza)

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  1. 1
    Staff View
    Publication Date:
    2016-02-11
    Publisher:
    Nature Publishing Group (NPG)
    Print ISSN:
    0028-0836
    Electronic ISSN:
    1476-4687
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Aquatic Organisms/genetics/isolation & purification/*metabolism ; Carbon/*metabolism ; Chlorophyll/metabolism ; Dinoflagellida/genetics/isolation & purification/metabolism ; *Ecosystem ; Expeditions ; Genes, Bacterial ; Genes, Viral ; Geography ; Oceans and Seas ; Photosynthesis ; Plankton/genetics/isolation & purification/*metabolism ; Seawater/*chemistry/microbiology/parasitology ; Synechococcus/genetics/isolation & purification/metabolism/virology
    Published by:
    Latest Papers from Table of Contents or Articles in Press
  2. 2
    Staff View
    Publication Date:
    2018-09-20
    Publisher:
    American Association for the Advancement of Science (AAAS)
    Electronic ISSN:
    2375-2548
    Topics:
    Natural Sciences in General
    Published by:
    Latest Papers from Table of Contents or Articles in Press
  3. 3
    Kuznar, J. ; Soler, M. ; Farias, G. ; Espinoza, J. C.
    Springer
    Published 1995
    Staff View
    ISSN:
    1432-8798
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Medicine
    Notes:
    Summary Infectious pancreatic necrosis virus (IPNV) attaches to CHSE-214 cells through two types of cell components: specific and non-specific ones. Competition experiments with inactivated IPNV showed that IPNV requires specific components to productively infect cells. Just a low amount of adsorbed IPNV enters the cell. After 20 minutes, part of the adsorbed IPNV was internalized into acid compartments. Also, the viruses adsorbed on the cell surface require similar periods of time to escape from the neutralization of antibodies.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  4. 4
    Espinoza, J. C. ; Hjalmarsson, A. ; Everitt, E. ; Kuznar, J.
    Springer
    Published 2000
    Staff View
    ISSN:
    1432-8798
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Medicine
    Notes:
    Summary.  The temporal subcellular localization of the structural proteins VP2 and VP3 of infectious pancreatic necrosis virus was analyzed with monoclonal antibodies conjugated with fluorochromes. Early in the infection both proteins were colocalized in the cytosol, at later times, VP2 was visualized as inclusion bodies around the nuclei of the cells and, sometimes, it was found in elongated tubular structures that might correspond to the type I tubules seen in cells infected with another Birnavirus. As VP2 is a glycosylated protein we determined its subcellular localization compared with that of ER and Golgi probes. These results suggest that VP2 is glycosylated freely in the cytoplasm.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  5. 5
    Espinoza, J. C. ; Kuznar, J.
    Springer
    Published 1997
    Staff View
    ISSN:
    1432-8798
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Medicine
    Notes:
    Summary.  The early events in the infection of unenveloped viruses are still rather unknown and puzzling. However, as in the case of enveloped viruses, the acid pH of endosomes can be important to trigger the virus entry into the cytosol. To test if the infectious pancreatic necrosis virus (IPNV), an unenveloped virus, requires acid endosomal pH to infect cells, we assayed the effect of Bafilomycin A1 on IPNV replication. Concentrations of the antibiotic which inhibited the endosomal acidification of the host cells were unable to affect IPNV replication in CHSE-214 cells; therefore, the acid pH of endosomes seems not to be a mandatory condition for the entry of IPNV into cells.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses