Search Results - (Author, Cooperation:J. Avila)
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1Latest Papers from Table of Contents or Articles in PressJ. S. Lee ; M. Hnilova ; M. Maes ; Y. C. Lin ; A. Putarjunan ; S. K. Han ; J. Avila ; K. U. Torii
Nature Publishing Group (NPG)
Published 2015Staff ViewPublication Date: 2015-06-18Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Arabidopsis/genetics/growth & development/*metabolism ; Arabidopsis Proteins/genetics/*metabolism ; *Binding, Competitive ; DNA-Binding Proteins/*metabolism ; Enzyme Activation ; Hypocotyl/metabolism ; MAP Kinase Signaling System ; Mitogen-Activated Protein Kinases/metabolism ; Phosphorylation ; Plant Stomata/*growth & development/*metabolism ; Protein-Serine-Threonine Kinases/deficiency/genetics/*metabolism ; Receptors, Cell Surface/deficiency/genetics/*metabolism ; Seedlings/enzymology/metabolism ; Transcription Factors/*metabolismPublished by: -
2Latest Papers from Table of Contents or Articles in PressD. Lu ; W. Lin ; X. Gao ; S. Wu ; C. Cheng ; J. Avila ; A. Heese ; T. P. Devarenne ; P. He ; L. Shan
American Association for the Advancement of Science (AAAS)
Published 2011Staff ViewPublication Date: 2011-06-18Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyComputer ScienceMedicineNatural Sciences in GeneralPhysicsKeywords: Amino Acid Motifs ; Amino Acid Sequence ; Arabidopsis/genetics/*immunology/metabolism/microbiology ; Arabidopsis Proteins/chemistry/genetics/*metabolism ; Flagellin/*immunology ; *Immunity, Innate ; Molecular Sequence Data ; Mutant Proteins/chemistry/metabolism ; Peptide Fragments/immunology ; Phosphorylation ; Plant Diseases/*immunology/microbiology ; Protein Interaction Domains and Motifs ; Protein Kinases/chemistry/*metabolism ; Protein-Serine-Threonine Kinases/*metabolism ; Pseudomonas syringae/growth & development/immunology ; Receptors, Pattern Recognition/chemistry/*metabolism ; Recombinant Fusion Proteins/metabolism ; Signal Transduction ; Ubiquitin-Protein Ligases/chemistry/genetics/*metabolism ; Ubiquitinated Proteins/metabolism ; UbiquitinationPublished by: -
3Latest Papers from Table of Contents or Articles in PressCasanova-Acebes, M., Nicolas-Avila, J. A., Li, J. L., Garcia-Silva, S., Balachander, A., Rubio-Ponce, A., Weiss, L. A., Adrover, J. M., Burrows, K., A-Gonzalez, N., Ballesteros, I., Devi, S., Quintana, J. A., Crainiciuc, G., Leiva, M., Gunzer, M., Weber, C., Nagasawa, T., Soehnlein, O., Merad, M., Mortha, A., Ng, L. G., Peinado, H., Hidalgo, A.
Rockefeller University Press
Published 2018Staff ViewPublication Date: 2018-11-06Publisher: Rockefeller University PressPrint ISSN: 0022-1007Electronic ISSN: 1540-9538Topics: MedicineKeywords: Innate Immunity and InflammationPublished by: -
4Articles: DFG German National LicensesStaff View
ISSN: 1471-4159Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Abstract: Whereas cells from most clonal lines derived from the murine neuroblastoma C1300 tumor can be induced to differentiate by serum withdrawal from culture medium, the NIA-103 clonal cell line has been considered unable to extend axon-like processes (neurites). Neurite growth depends on microtubule protein assembly, and although NIA-103 cells have essentially the same amounts of microtubule-associated protein (MAP)-1B and the neuronal-specific class β3-tubulin isoform as other neuroblastoma cell lines, these proteins are not phosphorylated in NIA-103 cells on serum withdrawal. The lack of microtubule protein phosphorylation may be due to the different sorting between the nucleus and the cytoplasm of the casein kinase II-related enzyme that is possibly involved in the modification of microtubule proteins. It is interesting that addition of DNA synthesis inhibitors to serum-starved NIA-103 cell cultures induces an increase in the level of cytosolic casein kinase II, an augmented in situ phosphorylation of MAP-1B, and the extension of neurites. Thus, the level of cytoplasmic casein kinase II appears to be controlled by the growth status of neuroblastoma cells. The shift to an increased cytoplasmic concentration of casein kinase II in nonproliferating, differentiating neuroblastoma cells is consistent with its putative role in the regulation of the cytoskeletal rearrangements underlying neuronal morphogenesis and plasticity.Type of Medium: Electronic ResourceURL: -
5Articles: DFG German National LicensesStaff View
ISSN: 1471-4159Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Abstract: The recent finding that several point mutations in the gene encoding for the microtubule-binding protein tau correlate with neurological disorders has heightened interest in the mechanisms of destabilization of this protein. In this study the functional consequences of the tau mutation R406W on the interaction of the protein with microtubules have been analyzed. Mutated tau is less phosphorylated than its normal counterpart at serines 396 and 404. Furthermore, the phosphorylated mutant protein is unable to bind to microtubules, and, as a consequence, microtubules assembled after transient nocodazole treatment in the presence of this tau variant contain only unmodified tau and appear to form more and longer bundles than those assembled in the presence of wild-type tau. We propose that phosphorylated tau, unbound to microtubules, could accumulate in the cytoplasm.Type of Medium: Electronic ResourceURL: -
6Articles: DFG German National LicensesSanchez, S. ; Sayas, C. L. ; Lim, F. ; Diaz-Nido, J. ; Avila, J. ; Wandosell, F.
Oxford, UK : Blackwell Science Ltd
Published 2001Staff ViewISSN: 1471-4159Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: It has been extensively described that neuronal differentiation involves the signalling through neurotrophin receptors to a Ras-dependent mitogen-activated protein kinase (MAPK) cascade. However, signalling pathways from other neuritogenic factors have not been well established. It has been reported that cAMP may activate protein kinase (PKA), and it has been shown that PKA-mediated stimulation of MAPK pathway regulates not only neuritogenesis but also survival. However, extracellular regulated kinases (ERKs) mediated pathways are not sufficient to explain all the processes which occur in neuronal differentiation. Our present data show that: in cAMP-mediated neuritogenesis, using the SH-SY5Y human neuroblastoma cell line, there exists a link between the activation of PKA and stimulation of phosphatidylinositol 3-kinase (PI3K). Both kinase activities are essential to the initial elongation steps. Surprisingly, this neuritogenic process appears to be independent of ERKs. While the activity of PI3K is essential for elongation and maintenance of neurites, its inhibition causes retraction. In this neurite retraction process, GSK3 is activated. Using both a pharmacological approach and gene transfer of a dominant negative form of GSK3, we conclude that this induced retraction is a GSK3-dependent process which in turn appears to be a common target for transduction pathways involved in lysophosphatidic acid-mediated and PI3K-mediated neurite retraction.Type of Medium: Electronic ResourceURL: -
7Articles: DFG German National LicensesSemaphorin 3C preserves survival and induces neuritogenesis of cerebellar granule neurons in cultureMoreno-Flores, M. T. ; Martín-Aparicio, E. ; Martín-Bermejo, M. J. ; Agudo, M. ; McMahon, S. ; Ávila, J. ; Díaz-Nido, J. ; Wandosell, F.
Oxford, UK : Blackwell Science Ltd
Published 2003Staff ViewISSN: 1471-4159Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Semaphorins (sema) constitute a family of molecules sharing a common extracellular domain (semaphorin domain). This family includes several types of secreted and membrane-associated molecules that are grouped into eight subclasses (subclasses 1–7 and viral semaphorins). Subclass 3 semaphorins are secreted molecules involved in axonal guidance, mainly through repulsive gradients and induction of growth cone collapse. More recently sema 3 molecules have been identified as positive factors in dependence of the type of neurons. Besides their axonal guidance function, some semaphorins have been implicated in apoptosis and survival. We investigated the effect of sema3C on survival and neurite outgrowth of rat cerebellar granule neurons (CGNs) in culture. 3T3 cells were stably transfected with sema3C. Several clonal lines were established and tested for their neuritogenic activity and one, S3C-8, was selected for the bulk of experiments. S3C-8 was co-cultured with CGNs. Sema3C enhanced CGN viability as assessed in co-cultures of CGNs with monolayers of S3C-8 in comparison with co-cultures of CGNs with control mock-transfected 3T3 cells. Moreover sema3C induced neuritogenesis of cultured CGNs, which express neuropilin-1 and -2. S3C-8 cells, overexpressing sema3C, were significantly more neuritogenic for CGN than poly l-lysine (PLL), a positive substrate for CGNs, as assessed by the measurement of the length of neurites and confirmed by Tau expression along the time of culture. CGNs co-cultured with S3C-8, showed up-regulation of the expression of axonal microtubule-associated proteins (MAPs) such as Tau, phosphorylated MAP2C and mode I-phosphorylated MAP1B compared with neurons cultured on control 3T3 cells. We also found increased expression of a specific marker of neuronal cell bodies and dendrites, high molecular weight MAP2 (HMW-MAP2). Interestingly, there was no accompanying up-regulation of a marker enriched within the neuronal somatodendritic domain, mode II-phosphorylated MAP1B. These data support the idea that secreted sema3C favors survival and neuritogenesis of cultured CGNs.Type of Medium: Electronic ResourceURL: -
8Articles: DFG German National LicensesStaff View
ISSN: 1077-3118Source: AIP Digital ArchiveTopics: PhysicsNotes: The reactivity of the Al/Si3N4/Si(100) system has been studied using x-ray photoelectron spectroscopy (XPS). The Si3N4 overlayer was prepared on Si(100) by N ion implantation and subsequent annealing. The deposition at 673 K of Al on a 18 A(ring) Si3N4 overlayer leads to the total reduction of the Si3N4 overlayer and the aluminum nitridation. The reaction also takes place at room temperature (RT) but to a lesser degree. The stability of a RT formed Al/Si3N4/Si structure was examined by increasing the sample temperature up to 673 K. In this way, the near complete reduction of a 24 A(ring) Si3N4 overlayer was obtained. These results show the instability of the Al/Si3N4 interface at moderate annealing temperatures. © 1995 American Institute of Physics.Type of Medium: Electronic ResourceURL: -
9Articles: DFG German National LicensesStaff View
ISSN: 1460-9568Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Neuronal microtubules have unique stability properties achieved through developmental regulation at the expression and post-translational levels on tubulins and microtubule associated proteins. One of the most specialized tubulins specific for neurons is class-III β-tubulin (also known as β6-tubulin). Both the upregulation and the post-translational processing of class-III β-tubulin are believed to be essential throughout neuronal differentiation. The present investigation documents the temporal and spatial patterns of class-III β-tubulin expression throughout neurogenesis. For this study a novel polyclonal antiserum named U-β6, specific to unphosphorylated class-III β-tubulin has been developed, characterized and compared with its commercial homologue TuJ-1. Our experiments indicate that the two antibodies recognize different forms of class-III β-tubulin both in vitro and in vivo. Biochemical data revealed that U-β6 bound unphosphorylated soluble class-III β-tubulin specifically, while TuJ-1 recognized both the phosphorylated and unphosphorylated forms of the denatured protein. In vivo U-β6 was associated with neurogenesis and labelled newly committed CNS and PNS neuroblasts expressing neuroepithelial cytoskeletal (nestin and vimentin) and surface markers (the anti-ganglioside supernatant, A2B5 and the polysialic acid neural adhesion molecule, PSA-NCAM), as well as differentiating neurons. These studies with U-β6 illustrate three main developmental steps in the neuronal lineage: the commitment of neuroepithelial cells to the lineage (U-β6 +ve/TuJ-1 –ve cells); a differentiation stage (U-β6 +ve/TuJ-1 +ve cells); and, finally, neuronal maturation correlating with a drop in unphosphorylated class-III β-tubulin immunostaining levels. These investigations also conclude that U-β6 is an earlier marker than TuJ-1 for the neuronal lineage in vivo, and it is thus the earliest neuronal lineage marker known so far.Type of Medium: Electronic ResourceURL: -
10Articles: DFG German National LicensesStaff View
ISSN: 0304-4165Keywords: (Porcine) ; Immunofluorescence ; Tau factorSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyMedicinePhysicsType of Medium: Electronic ResourceURL: -
11Articles: DFG German National LicensesStaff View
ISSN: 0006-291XKeywords: [abr] ELISA; enzyme-linked immunoabsorbent assay ; [abr] NP-40; Nonidet P-40 ; [abr] PBS; phosphate-buffered saline ; [abr] SDS-PAGE; sodium dodecyl sulphate polyacrylamide gelSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyPhysicsType of Medium: Electronic ResourceURL: -
12Articles: DFG German National LicensesStaff View
ISSN: 0006-291XKeywords: [abr] EGTA; ethyleneglycol-bis-( β-amino ethyl ether) ; [abr] MES; 2-(N-morpholino) ethane sulfonic acidSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyPhysicsType of Medium: Electronic ResourceURL: -
13Articles: DFG German National LicensesStaff View
ISSN: 0005-2736Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyMedicinePhysicsType of Medium: Electronic ResourceURL: -
14Articles: DFG German National LicensesStaff View
ISSN: 0003-9861Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyPhysicsType of Medium: Electronic ResourceURL: -
15Articles: DFG German National LicensesStaff View
ISSN: 0003-9861Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyPhysicsType of Medium: Electronic ResourceURL: -
16Articles: DFG German National LicensesStaff View
ISSN: 0003-9861Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyPhysicsType of Medium: Electronic ResourceURL: -
17Articles: DFG German National LicensesStaff View
ISSN: 0003-9861Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyPhysicsType of Medium: Electronic ResourceURL: -
18Articles: DFG German National LicensesStaff View
ISSN: 0006-291XSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyPhysicsType of Medium: Electronic ResourceURL: -
19Articles: DFG German National LicensesStaff View
ISSN: 0005-2744Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyType of Medium: Electronic ResourceURL: -
20Articles: DFG German National LicensesStaff View
ISSN: 0005-2787Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyType of Medium: Electronic ResourceURL: