Search Results - (Author, Cooperation:J. Audet)

2014-08-30

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Amino Acid Sequence ; Animals ; Antibodies, Monoclonal/immunology/*therapeutic use ; Antibodies, Neutralizing/immunology/therapeutic use ; Antibodies, Viral/immunology/*therapeutic use ; Cross Reactions/immunology ; Ebolavirus/immunology ; Enzyme-Linked Immunosorbent Assay ; Female ; Guinea ; Guinea Pigs ; Hemorrhagic Fever, Ebola/blood/*drug therapy/immunology/virology ; *Immunization, Passive ; Macaca mulatta/immunology/virology ; Male ; Molecular Sequence Data ; Sequence Alignment ; Viral Envelope Proteins/chemistry/immunology ; Viremia/drug therapy/immunology/virology

2018-03-15

American Association for the Advancement of Science (AAAS)

2375-2548

Natural Sciences in General

1432-0797

Springer Online Journal Archives 1860-2000

Process Engineering, Biotechnology, Nutrition Technology

Abstract Reciprocating plate bioreactors are particularly well suited for conducting fermentations which give rise to highly viscous broth. To evaluate their performance for polysaccharide fermentations, a series of pullulan fermentations were performed with a particular emphasis placed on the influence of aeration on both the quantity and quality of the product. Two experiments were conducted at constant aeration rates and two others with constant dissolved oxygen concentrations. For the latter two experiments, the dissolved oxygen concentration was controlled by manipulating either the aeration flow rate or the reciprocating frequency of the perforated plates. It was found that, in general, a higher dissolved oxygen concentration leads to a higher productivity but the quality of the product, expressed in terms of the viscosity of the fermentation broth, was nevertheless reduced. It appears that the optimum yield, in terms of both quantity and quality, would be achieved at an intermediate dissolved oxygen concentration.

Electronic Resource

1432-0797

Springer Online Journal Archives 1860-2000

Process Engineering, Biotechnology, Nutrition Technology

Abstract The environment in which live microorganisms has a major impact on their productivity. One important factor is the mechanical mixing that is used to promote good heat and mass transfer in bioreactors. In this paper, the performance of reciprocating plate bioreactors is first evaluated for their ability to produce high oxygen transfer coefficients. Pure water and a glycerol water (50∶50 wt%) solution are used for this evaluation. Then, the performance of reciprocating plate bioreactors for the production of an exocellular polysaccharide (pullulan) by yeast Aureobasidium pullulans is analyzed in terms of quantity and quality of the polysaccharide. Results clearly show that a more efficient substrate utilisation is achieved with reciprocating plate bioreactors.

Electronic Resource

0040-5639

Theology and Religious Studies

BOOK REVIEWS

0022-5185

Theology and Religious Studies

0178-515X

Springer Online Journal Archives 1860-2000

Process Engineering, Biotechnology, Nutrition Technology

Abstract This investigation was undertaken with the objective to compare experimentally the performance of four different mixing devices for the production of the polysaccharide pullulan with Aureobasidium pullulans (2552). Fermentations were performed using identical bioreactors with respectively an assembly of three Rushton turbines (RTB), a helical ribbon impeller (HR) and two different reciprocating plates (RPB1, RPB2). Each mixing vessel had identical geometry and working volume (18 L). These four fermentations were performed with an equal level of power input per unit volume (1000 W/m3) and gas flow rate (0.5 vvm, 9 L/min). For each system, the evolution of biomass, polysaccharide concentration, dissolved oxygen and agitation speed or frequency were recorded as a function of time along with the rheological properties of the culture broths. The type of mixing device used had a significant impact on the rate of biomass production and on polysaccharide physical properties. However, the rate of polysaccharide production appears to be less sensitive to the bioreactor design. The overall productivity, which represents the ability of micro-organisms to convert rapidly substrate into biomass and polysaccharide, was maximised using the RPB2 system. The quality of the synthesised polysaccharide, in terms of viscosity producing power, was highest using the HR system but the overall productivity was very low. Thus, the best compromise between productivity and product quality was achieved with the RPB2.

Electronic Resource

0178-515X

Springer Online Journal Archives 1860-2000

Process Engineering, Biotechnology, Nutrition Technology

Abstract  The environment in which live microorganisms has a major impact on their productivity. One important factor is the mechanical mixing that is used to promote good heat and mass transfer in bioreactors. In this paper, the performance of reciprocating plate bioreactors is first evaluated for their ability to produce high oxygen transfer coefficients. Pure water and a glycerol water (50:50 wt%) solution are used for this evaluation. Then, the performance of reciprocating plate bioreactors for the production of an exocellular polysaccharide (pullulan) by yeast Aureobasidium pullulans is analyzed in terms of quantity and quality of the polysaccharide. Results clearly show that a more efficient substrate utilisation is achieved with reciprocating plate bioreactors.

Electronic Resource

0178-515X

Springer Online Journal Archives 1860-2000

Process Engineering, Biotechnology, Nutrition Technology

Abstract  Reciprocating plate bioreactors are particularly well suited for conducting fermentations which give rise to highly viscous broth. To evaluate their performance for polysaccharide fermentations, a series of pullulan fermentations were performed with a particular emphasis placed on the influence of aeration on both the quantity and quality of the product. Two experiments were conducted at constant aeration rates and two others with constant dissolved oxygen concentrations. For the latter two experiments, the dissolved oxygen concentration was controlled by manipulating either the aeration flow rate or the reciprocating frequency of the perforated plates. It was found that, in general, a higher dissolved oxygen concentration leads to a higher productivity but the quality of the product, expressed in terms of the viscosity of the fermentation broth, was nevertheless reduced. It appears that the optimum yield, in terms of both quantity and quality, would be achieved at an intermediate dissolved oxygen concentration.

Electronic Resource