Search Results - (Author, Cooperation:J. A. Scheffler)
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1Latest Papers from Table of Contents or Articles in PressA. H. Paterson ; J. F. Wendel ; H. Gundlach ; H. Guo ; J. Jenkins ; D. Jin ; D. Llewellyn ; K. C. Showmaker ; S. Shu ; J. Udall ; M. J. Yoo ; R. Byers ; W. Chen ; A. Doron-Faigenboim ; M. V. Duke ; L. Gong ; J. Grimwood ; C. Grover ; K. Grupp ; G. Hu ; T. H. Lee ; J. Li ; L. Lin ; T. Liu ; B. S. Marler ; J. T. Page ; A. W. Roberts ; E. Romanel ; W. S. Sanders ; E. Szadkowski ; X. Tan ; H. Tang ; C. Xu ; J. Wang ; Z. Wang ; D. Zhang ; L. Zhang ; H. Ashrafi ; F. Bedon ; J. E. Bowers ; C. L. Brubaker ; P. W. Chee ; S. Das ; A. R. Gingle ; C. H. Haigler ; D. Harker ; L. V. Hoffmann ; R. Hovav ; D. C. Jones ; C. Lemke ; S. Mansoor ; M. ur Rahman ; L. N. Rainville ; A. Rambani ; U. K. Reddy ; J. K. Rong ; Y. Saranga ; B. E. Scheffler ; J. A. Scheffler ; D. M. Stelly ; B. A. Triplett ; A. Van Deynze ; M. F. Vaslin ; V. N. Waghmare ; S. A. Walford ; R. J. Wright ; E. A. Zaki ; T. Zhang ; E. S. Dennis ; K. F. Mayer ; D. G. Peterson ; D. S. Rokhsar ; X. Wang ; J. Schmutz
Nature Publishing Group (NPG)
Published 2012Staff ViewPublication Date: 2012-12-22Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Alleles ; *Biological Evolution ; Cacao/genetics ; Chromosomes, Plant/genetics ; *Cotton Fiber ; Diploidy ; Gene Duplication/genetics ; Genes, Plant/genetics ; Genome, Plant/*genetics ; Gossypium/classification/*genetics ; Molecular Sequence Annotation ; Phylogeny ; *Polyploidy ; Vitis/geneticsPublished by: -
2Articles: DFG German National LicensesStaff View
ISSN: 1439-0523Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: The effectiveness of 200- and 400-m isolation distances were evaluated for small-scale trials of oilseed rape (Brassica napus L.). These distances have been used for previous transgenic release experiments and are commonly adopted for production of basic and certified breeders seed. A 400-m2 donor plot contained plants with a dominant transgene conferring resistance to the herbicide glufosinate-ammonium. This character was used as a selectable marker to measure the frequency of hybrid formation in 400-m2 target plots of non-transgenic rape. Seeds produced by the non-transgenic plants were permitted to fall onto the plots and allowed to germinate. At the first true-leaf stage, the plants were sprayed with glufosinate-ammonium. Surviving plants were resprayed, and a subsample assayed for the presence of the transgene using a colorimetric assay and Southern-blot analysis. The average frequency of hybridization over two replicates was 0.0156% at 200 m and 0.0038% at 400 m. These estimates are within the limits established for the production of basic seed (0.1%). Results indicated that bees were the most likely agent for long-distance pollen dispersal.Type of Medium: Electronic ResourceURL: -
3Articles: DFG German National LicensesStaff View
ISSN: 1439-0523Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: With advances in recombinant DNA methods and transformation procedures, it is possible to transfer genes into crop plants from unrelated plants, microbes and animals. Many of the modifications being carried out, or envisaged, are for disease and pest resistance, product quality and tolerance to environmental stress, but there are additional opportunities to modify crops to give specialized products for industrial or pharmaceutical use. Some of the characteristics of transgenic plants are considered, including: transgene copy number, position, expression, stability, pleiotropy, selectable marker genes and somaclonal variation. There have been several hundreds of field trials with transgenic plants, and the first transgenic varieties are likely to be approved for commercial production in 1993. Before releasing transgenic plants, it is necessary to carry out a risk assessment to determine whether the transgenic variety will behave differently from a conventionally bred variety. Assessment procedures are being harmonized internationally by various organizations. There is a growing commitment to apply these genetic modification methods to crops in developing countries, as genes relevant to their crops and environments become available.Type of Medium: Electronic ResourceURL: -
4Articles: DFG German National LicensesScheffler, J. A. ; Sharpe, A. G. ; Schmidt, H. ; Sperling, P. ; Parkin, I. A. P. ; Lühs, W. ; Lydiate, D. J. ; Heinz, E.
Springer
Published 1997Staff ViewISSN: 1432-2242Keywords: Key words Brassica napus ; Collinearity ; Desaturation ; Fatty acid ; Genetic mappingSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract This paper reports the estimated gene copy number and restriction fragment length polymorphism (RFLP) map locations of five different desaturase cDNA clones from Brassica napus (oilseed rape). The desaturase enzymes encoded by four of these genes catalyze successive reactions that insert double bonds into lipid-linked fatty acid residues. Delta-12 (e2) and delta-15 (e3) desaturases are active in the endoplasmic reticulum, while omega-6 (p2) and omega-3 (p3) desaturases catalyze analogous desaturation reactions via a parallel pathway located in plastids. The fifth cDNA clone (b5) contains a desaturase-like domain bound to a cytochrome b5 segment. Estimates of gene copy number based on Southern blot analysis of 16 oilseed rape varieties and three different resynthesized Brassica napus lines indicated that e2 had 4–6 gene copies and e3, p2, p3 and b5 each had 6–8 gene copies per haploid genome. Estimates of the gene copy number for the two progenitor species, Brassica oleracea and Brassica rapa, supported the premise that all these genes were at least duplicated or triplicated in the two progenitor species before they combined to form B. napus. RFLP mapping results showed that the e2 probe detected 4 distinct loci, the e3 probe 6 loci and p2, p3 and b5 each detected 8 loci, with pairs of loci often mapping to homoeologous regions on 2 different linkage groups. The 28 mappable loci were distributed across 12 linkage groups of the B. napus map (Parkin et al. 1995) and were usually represented by single RFLP fragments. A collinear segment containing the e2 and p3 loci was positioned on B. napus linkage groups N1, N11, N3, N13, N5 and N15. This segment was collinear with a 30-cM region of Arabidopsis thaliana chromosome 3 that contains the homologous fad2 (e2) and fad7(p3) genes. This suggests that the desaturase multigene families arose as the result of duplication of large chromosome segments rather than duplication of individual genes.Type of Medium: Electronic ResourceURL: