Search Results - (Author, Cooperation:I. Treilleux)

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  1. 1
    S. Nik-Zainal ; H. Davies ; J. Staaf ; M. Ramakrishna ; D. Glodzik ; X. Zou ; I. Martincorena ; L. B. Alexandrov ; S. Martin ; D. C. Wedge ; P. Van Loo ; Y. S. Ju ; M. Smid ; A. B. Brinkman ; S. Morganella ; M. R. Aure ; O. C. Lingjaerde ; A. Langerod ; M. Ringner ; S. M. Ahn ; S. Boyault ; J. E. Brock ; A. Broeks ; A. Butler ; C. Desmedt ; L. Dirix ; S. Dronov ; A. Fatima ; J. A. Foekens ; M. Gerstung ; G. K. Hooijer ; S. J. Jang ; D. R. Jones ; H. Y. Kim ; T. A. King ; S. Krishnamurthy ; H. J. Lee ; J. Y. Lee ; Y. Li ; S. McLaren ; A. Menzies ; V. Mustonen ; S. O'Meara ; I. Pauporte ; X. Pivot ; C. A. Purdie ; K. Raine ; K. Ramakrishnan ; F. G. Rodriguez-Gonzalez ; G. Romieu ; A. M. Sieuwerts ; P. T. Simpson ; R. Shepherd ; L. Stebbings ; O. A. Stefansson ; J. Teague ; S. Tommasi ; I. Treilleux ; G. G. Van den Eynden ; P. Vermeulen ; A. Vincent-Salomon ; L. Yates ; C. Caldas ; L. V. Veer ; A. Tutt ; S. Knappskog ; B. K. Tan ; J. Jonkers ; A. Borg ; N. T. Ueno ; C. Sotiriou ; A. Viari ; P. A. Futreal ; P. J. Campbell ; P. N. Span ; S. Van Laere ; S. R. Lakhani ; J. E. Eyfjord ; A. M. Thompson ; E. Birney ; H. G. Stunnenberg ; M. J. van de Vijver ; J. W. Martens ; A. L. Borresen-Dale ; A. L. Richardson ; G. Kong ; G. Thomas ; M. R. Stratton
    Nature Publishing Group (NPG)
    Published 2016
    Staff View
    Publication Date:
    2016-05-03
    Publisher:
    Nature Publishing Group (NPG)
    Print ISSN:
    0028-0836
    Electronic ISSN:
    1476-4687
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Natural Sciences in General
    Physics
    Published by:
    http://www.nature.com/

    Latest Papers from Table of Contents or Articles in Press
  2. 2
    Angèle, S ; Treilleux, I ; Brémond, A ; Tanière, P ; Hall, J

    Oxford, UK : Blackwell Science Ltd
    Published 2003
    Staff View
    ISSN:
    1365-2559
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Medicine
    Notes:
    Aims:  To determine whether the expression of DNA damage detection and repair proteins is frequently altered in breast carcinomas.Methods and results:  The expression profiles of five such proteins: ATM, p53, NBS1, MRE11 and Rad50 were analysed in 99 in-situ and invasive ductal breast carcinomas of different grades using an immunohistochemical approach, and compared with those seen in eight independent non-cancer (normal) breast samples and in the surrounding normal tissues of the breast carcinomas examined. ATM protein expression was reduced in 75% of the tumours compared with the levels found in normal tissues. Fewer tumours had reduced protein levels of the members of the MRE11, NBS1 and Rad50 (MNR) complex (31%, 46% and 28%, respectively) with p53 being over-expressed in 30%. In the majority of tumours (92%) we observed a good correlation between the expression of the three proteins of the MNR complex with low NBS1, MRE11 or Rad50 expression rarely found alone, suggesting that this event occurs subsequently to the deregulation in expression of other DNA repair proteins.Conclusion:  The pattern of protein changes observed supports our hypothesis that alterations in DNA double-strand break repair capacity are involved in mammary carcinogenesis.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1046/j.1365-2559.2003.01713.x

    Articles: DFG German National Licenses
  3. 3
    Staff View
    ISSN:
    1365-2559
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Medicine
    Notes:
    Aims:  HER2 protein is over-expressed in 15–30% of breast carcinomas. Immunohistochemistry (IHC) is a common and inexpensive method able to specifically detect HER2 protein. However, lack of standardization of IHC has been considered responsible for discrepancies in HER2 status assessment performed by IHC and fluorescence in-situ hybridization (FISH). This prompted us to perform a multicentric IHC calibration test to achieve a maximum accuracy of HER2-IHC compared with HER2-FISH taken as the reference method.Methods and results:  Twelve French laboratories participated in this study, including 119 cases of invasive breast carcinomas for which both fixed and frozen tissues were available. HER2 expression was determined in fixed tissues by individual in-house IHC techniques, using either CB11 (Novocastra, Newcastle, UK) or A0485 (Dako, Glostrup, Denmark) anti-HER2 antibodies. Two cut-off values were used: 10% and 60% of immunostained cells. In 116 of the 119 cases, HER2 gene status could also be determined by FISH on frozen sections, performed in a single laboratory. Results were centralized and compared. When suboptimal concordance between IHC and FISH was observed, IHC was calibrated and a second run was performed. The specificity, sensitivity and accuracy of IHC compared with FISH were noted before and after calibration. Forty-four out of 116 (38%) tumours showed HER2 gene amplification. Accuracy of IHC was complete in the first run for 6/12 laboratories. Calibration, necessary for the six others, relied mainly on the combination of a heat-induced epitope retrieval step with an increase of dilution of the primary antibody. In the second run, HER2 over-expression was found in 46 (40%) and 44 (38%) of the 116 cases, using 10% or 60% of stained cells as cut-offs, respectively. The corresponding accuracy rates were 93% and 95%.Conclusions:  This study showed that a high accuracy of IHC could be obtained for the determination of HER2 status in all laboratories using their in-house IHC technique, provided that a calibration process was performed. Antigen retrieval procedure, high dilutions of anti-HER2 antibody and the use of specific controls were crucial for HER2-IHC calibration. A 95% accuracy rate of IHC, using FISH as gold standard, was obtained by considering immunolabelling HER2-IHC results as a continuous variable, and taking 60% invasive stained cells as the cut-off for HER2 over-expression.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1046/j.1365-2559.2003.01598.x

    Articles: DFG German National Licenses