Search Results - (Author, Cooperation:I. Petersen)
-
1Latest Papers from Table of Contents or Articles in PressJ. George ; J. S. Lim ; S. J. Jang ; Y. Cun ; L. Ozretic ; G. Kong ; F. Leenders ; X. Lu ; L. Fernandez-Cuesta ; G. Bosco ; C. Muller ; I. Dahmen ; N. S. Jahchan ; K. S. Park ; D. Yang ; A. N. Karnezis ; D. Vaka ; A. Torres ; M. S. Wang ; J. O. Korbel ; R. Menon ; S. M. Chun ; D. Kim ; M. Wilkerson ; N. Hayes ; D. Engelmann ; B. Putzer ; M. Bos ; S. Michels ; I. Vlasic ; D. Seidel ; B. Pinther ; P. Schaub ; C. Becker ; J. Altmuller ; J. Yokota ; T. Kohno ; R. Iwakawa ; K. Tsuta ; M. Noguchi ; T. Muley ; H. Hoffmann ; P. A. Schnabel ; I. Petersen ; Y. Chen ; A. Soltermann ; V. Tischler ; C. M. Choi ; Y. H. Kim ; P. P. Massion ; Y. Zou ; D. Jovanovic ; M. Kontic ; G. M. Wright ; P. A. Russell ; B. Solomon ; I. Koch ; M. Lindner ; L. A. Muscarella ; A. la Torre ; J. K. Field ; M. Jakopovic ; J. Knezevic ; E. Castanos-Velez ; L. Roz ; U. Pastorino ; O. T. Brustugun ; M. Lund-Iversen ; E. Thunnissen ; J. Kohler ; M. Schuler ; J. Botling ; M. Sandelin ; M. Sanchez-Cespedes ; H. B. Salvesen ; V. Achter ; U. Lang ; M. Bogus ; P. M. Schneider ; T. Zander ; S. Ansen ; M. Hallek ; J. Wolf ; M. Vingron ; Y. Yatabe ; W. D. Travis ; P. Nurnberg ; C. Reinhardt ; S. Perner ; L. Heukamp ; R. Buttner ; S. A. Haas ; E. Brambilla ; M. Peifer ; J. Sage ; R. K. Thomas
Nature Publishing Group (NPG)
Published 2015Staff ViewPublication Date: 2015-07-15Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsPublished by: -
2Articles: DFG German National LicensesFalk-Petersen, I-B. ; Hansen, T. K. ; Fieler, R. ; Sunde, L. M.
Oxford, UK : Blackwell Science Ltd
Published 1999Staff ViewISSN: 1365-2109Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: The spotted wolffish Anarhichas minor (Olafsen), with its rich and tasty fillets, popular skin and high growth rates in captivity, is a promising candidate for cold-water aquaculture. We have established a production line for the wolffish during 5 years of active research on the biology of the species. Our broodstock, sampled from the Barents Sea, has mainly spawned during autumn. The females must be stripped as soon as possible after ovulation. The best egg batches have shown c. 100% fertilization and survival rates between 60% and 80% during the 800- to 960-daydegrees-long incubation period. Rearing temperatures of 6 °C, 8 °C, ambient and decreasing temperatures have turned out to be satisfactory for survival. Treatment with 150 p.p.m. glutardialdehyde twice a month is recommended to control microorganism growth on eggs. Premature hatching has been a problem in individual egg batches; the reasons are not fully understood. Normally hatched individuals are ≈ 22 mm long and well developed, with a small yolk sac, which is completely resorbed after about 4 weeks at 6–8 °C. The fry have been successfully start fed on formulated feed as well as Artemia. Highest early growth rates have been noted at 8 °C, but overall survival was best at 6 °C. On-growth of spotted wolffish juveniles fed formulated dry floating feed at low temperatures in shallow raceways has been promising. After 2, 3 and 4 years, the mean weights of the first generation produced in aquaculture were 0.7, 2.7 and 5.1 kg respectively. These individuals now make up the broodstock at a recently established commercial production facility.Type of Medium: Electronic ResourceURL: -
3Articles: DFG German National LicensesStaff View
ISSN: 1365-2109Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: Temperature influenced the developmental rate, survival and early growth of eggs and embryos of spotted wolffish, Anarhichas minor (Olafsen), an interesting candidate for cold water cultivation. The total incubation period decreased from 220 days at 4 °C (880 daydegrees), to 177 days at 6 °C (1062 daydegrees) and 150 days at 8 °C (1200 daydegrees) in these experiments. The proportion of normal embryos and survival of eggs until hatching were highest when the eggs were incubated at 6 °C. During the incubation period, the embryo and yolk sac size at 280 daydegrees was not significantly different but at 850 daydegrees the embryo size was inversely related to temperature and the remaining yolk sac size positively correlated with the incubation temperature. The transformation of yolk to body mass during incubation appeared to be most efficient at 4 °C, and the embryos hatched with a larger visible yolk sac at 6 and 8 °C. The largest larvae (wet-weight) hatched from the largest eggs and the egg groups incubated at the lowest temperature (4 °C). There was no effect of temperature on meristic characters. During 6 weeks post-hatching, all larvae from the three temperature groups were fed formulated dry feed in excess at 8 °C in low water-level raceway systems. During startfeeding, the larvae from eggs incubated at the lowest temperature (4 °C) showed the highest growth rates (SGR). Best survival of larvae was noted among batches incubated at 6 °C.Type of Medium: Electronic ResourceURL: -
4Articles: DFG German National LicensesStaff View
ISSN: 1095-8649Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: BiologyNotes: Wolffish Anarhichas lupus (Anarhichadidae) hatch from large demersal eggs (c. 5–6 mm) after a long incubation period (c. 800–1000 degree days) at 20–25 mm LT with a juvenile appearance: a small yolk sac, pigmented but partly transparent skin densely supplied with mucous cells, well developed body musculature and a cartilaginous skeleton. The results of histomorphological studies illustrate the apparent functionality of tissues and organs at hatching. The mucosa of the oral cavity and various parts of the digestive channel are well differentiated and the cellular characteristics are described within each region. A stomach with gastric glands is established before hatching. The pancreas has well developed endo- and exocrine tissue and the hepatocytes are vacuolized. The kidney tissue has numerous tubuli and haematopoietic tissue in the bilobed head region. The spleen, thymus and thyroid glands also appear mature. Cones are dominant in the visual cell layer of the retina.Type of Medium: Electronic ResourceURL: -
5Articles: DFG German National LicensesStaff View
ISSN: 1095-8649Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: BiologyNotes: Metamorphosis in cod, Gadus morhua L., was investigated with respect to morphological changes in the alimentary tract (mouth, stomach and pyloric caeca) and external characters (finfold and fin development). Morphometric measurements and histological investigations were made on both larvae and juveniles and all fish lengths are given as standard length. Median unpaired fins started to develop when the fish were 9–10 mm in length. The larval median finfold was resorbed when fish reached a size of 11–15 mm. The vertebrae were formed in fish of 11–20 mm. The supraterminal mouth, seen in larvae 〈12 mm, changed gradually to a sub-terminal position in juveniles larger than 65 mm. The development of the stomach and pyloric caeca started at 15 mm and the juvenile form was seen in fish of 40 mm. The ability to store and grind food particles in the stomach developed gradually as fish increased in size from 20 to 40 mm. Compared to many other teleost species, cod juveniles are relatively large when the stomach and pyloric caeca become fully developed. These findings may have important implications for both start-feeding of cod larvae and the weaning of juveniles on to artificial diets.Type of Medium: Electronic ResourceURL: -
6Articles: DFG German National LicensesStaff View
ISSN: 0003-2697Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyType of Medium: Electronic ResourceURL: -
7Articles: DFG German National LicensesBreitbart, E.W. ; Weyer, U. ; Petersen, I. ; Ehrke, C. ; Piwowarczyk, S. ; Kowalzick, L.
Amsterdam : ElsevierStaff ViewISSN: 0011-2240Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyMedicineType of Medium: Electronic ResourceURL: -
8Articles: DFG German National LicensesKnösel, T ; Schlüns, K ; Stein, U ; Schwabe, H ; Schlag, P M ; Dietel, M ; Petersen, I
Oxford, UK : Blackwell Science Ltd
Published 2003Staff ViewISSN: 1365-2559Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Aims: To investigate the prognostic significance of chromosomal alterations in colorectal cancer patients. Histopathological tumour classification is still considered to be the gold standard for the characterization of solid tumours. However, it is well known that such established parameters do not satisfactorily predict the clinical outcome in individual cases. Markers that reliably predict survival are needed. These markers should guide the clinical treatment of neoplastic disease.Methods and results: Chromosomal imbalances in 61 colorectal carcinoma specimens in 37 patients determined by comparative genomic hybridization were correlated with patient survival using custom-made computer software which enabled the assessment of individual chromosomal loci. Kaplan–Meier analysis revealed that over-representations of 2p14-15, 6q23-6q24, 15q22-15q23, 22q11.2 and deletions of 1p36.1-36.2, 4q31.3, 4q35, 8q12-q21, 8p11.2 and 9p22 were significantly associated with shorter disease-specific survival, whereas over-expression of 20q13.3 and deletion of 18q11.2 were significantly associated with longer disease-specific survival in this collection of colorectal cancers. Multivariate Cox proportional hazards regression models consistently identified gains of 2p14-15, 15q22-23, 22q11.2 and losses of 1p36.1-36.2 and 4q35 as independent markers of shorter patient survival carrying greater significance than the classical clinicopathological parameters of nodal status and tumour grade.Conclusions: These five markers allow a molecular categorization of patients into high and low clinical risk groups. Thus, the genomic data have refined the histopathological classification highlighting the necessity for a supplementary genetically based stratification of colorectal cancer.Type of Medium: Electronic ResourceURL: -
9Articles: DFG German National LicensesStaff View
ISSN: 0020-711XSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyType of Medium: Electronic ResourceURL: -
10Articles: DFG German National LicensesStaff View
ISSN: 0016-6480Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyMedicineType of Medium: Electronic ResourceURL: -
11Articles: DFG German National LicensesStaff View
ISSN: 0305-0491Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyType of Medium: Electronic ResourceURL: -
12Articles: DFG German National LicensesStaff View
ISSN: 0305-0491Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyType of Medium: Electronic ResourceURL: -
13Articles: DFG German National LicensesStaff View
ISSN: 0305-0491Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyType of Medium: Electronic ResourceURL: -
14Articles: DFG German National LicensesLipid class and fatty acid composition of eggs from the Atlantic halibut (Hippoglossus hippoglossus)Staff View
ISSN: 0044-8486Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionType of Medium: Electronic ResourceURL: -
15Articles: DFG German National LicensesStaff View
ISSN: 1420-9071Source: Springer Online Journal Archives 1860-2000Topics: BiologyMedicineNotes: Zusammenfassung 14C-Orotsäure wurde mit zwei verschiedenen Injektionsmethoden in Mäuse injiziert und die Serumaktivität nach 2, 5, 10, 20 und 60 min gemessen. Nach i.p. Injektion wurde Blut, das aus der Bauchhöle nach Entfernung der Leber entnommen worden war, mit noch nicht absorbiertem Isotop gemischt. Bei i.v. Schwanzinjektion verblieb bei nicht perfekter Injektion eine erhebliche Menge der Orotsäure im Schwanzgewebe und ergab einen kontinuierlichen Isotopenzuschuss zum Serum.Type of Medium: Electronic ResourceURL: -
16Articles: DFG German National LicensesStaff View
ISSN: 1432-1793Source: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract Seasonal lipid analyses were performed on the gonads and residual bodies of Ctenodiscus crispatus (Retzius) and Asterias lincki (Müller & Troschel), collected from Balsfjorden, northern Norway during March 1978–February 1980. The ovaries of both species had high percentages of total lipid (in the region of 60% of the dry weight) that were relatively constant throughout the season. Testes from both species had in the region of 20% of their dry weight as lipid, again with little seasonal variation. The majority of the total lipid in the ovaries of C. crispatus was accounted for by alkyldiacylglycerols and there was little seasonal variation in lipid class composition. Phospholipid accounted for the bulk of the lipid in the testes of C. crispatus, where alkyldiacylglycerols were present but in minor amounts. A slight degree of seasonal variation occurred in the composition of testis lipid in that maximal percentages of triacylglycerols and alkyldiacylglycerols occurred in January–April. The ovaries of A. lincki contained both triacylglycerols and alkyldiacylglycerols as major neutral lipids. Significant seasonal variation occurred in lipid class composition in the ovaries in that neutral lipids showed transient minima in February–April. The testes of A. lincki had substantial amounts of both triacylglycerols and alkyldiacylglycerols: maximal percentages of these lipids occurred in January–March. Analyses of gonads of near-mature Pteraster militaris (O. F. Müller) revealed that the ovaries were rich in lipid that was predominantly alkyldiacylglycerols, whereas the testes had much lower levels of lipid accounted for mainly by phospholipids. The residual bodies of all three asteroids had significant but small amounts of alkyldiacylglycerols in their lipids. A. lincki had triacylglycerols as a major lipid class in its body lipids, whereas in C. crispatus the major body lipid was phospholipid. Little seasonal variation was seen in the composition of the residual body lipid of either C. crispatus or A. lincki. The results are consistent with an aseasonal reproductive pattern in C. crispatus, although a main breeding activity occurred in mid-winter. A. lincki is a more seasonal breeder, but also had maximal activity in winter. Irrespective of their breeding habits, all three asteroids produced eggs rich in alkyldiacylglycerols. A major role for this lipid in the development of the large lecithotrophic eggs of asteroids is indicated.Type of Medium: Electronic ResourceURL: -
17Articles: DFG German National LicensesStaff View
ISSN: 1432-1793Source: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract Fatty acid analyses of lipids in the asteroids Ctenodiscus crispatus (Retzius), Asterias lincki (Müller and Troschel) and Pteraster militaris (O. F. Müller) collected in Balsfjorden, near Tromsø, northern Norway in 1978–1980, were performed to further our understanding of the asteroids' reproductive biology. Fatty acid compositions were determined in phospholipids, triacylglycerols and alkyldiacylglycerols isolated from ovaries of frozen C. crispatus. The major polyunsaturates in phospholipids were 20:4(n-6) followed by 20:5(n-3). Both the triacylglycerols and alkyldiacylglycerols were relatively deficient in polyunsaturates but had 20:1(n-13) and 18:1(n-7) as major components. Both these liqid classes had additionally 15% of their fatty acids as a variety of odd-numbered and branched-chain fatty acids. The fatty acid composition of neutral glycerides, largely alkyldiacylglycerols, from heat-dried ovaries of C. crispatus was not materially different from that of alkyldiacylglycerols isolated from the ovaries of frozen individuals. The fatty acid composition of neutral glycerides, largely alkyldiacylglycerols, from heat-dried ovaries of P. militaris was similar to that for C. crispatus in that 20:1(n-13) and 18:1(n-7) were major components, and odd-numbered together with branched-chain fatty acids accounted for about 15% of the total fatty acids. The fatty acid composition of neutral glycerides, consisting of equal amounts of triacylglycerols and alkyldiacylglycerols, from heat-dried ovaries of A. lincki differed from those of the previous two species in that 20:1(n-9) and 18:1(n-9) were major components and odd-numbered together with branched-chain fatty acids accounted for less than 5% of the total fatty acids. The results are discussed mainly in terms of lipid biosynthetic mechanisms within the asteroids and the interplay between internal biosynthetic activities and dietary fatty acids, particularly those from bacterial sources, is considered.Type of Medium: Electronic ResourceURL: -
18Articles: DFG German National LicensesStaff View
ISSN: 1433-0458Keywords: Schlüsselwörter Plattenepithelkarzinom ; Kopf-Hals-Bereich ; CGH ; Prognose ; Cox-Regression ; Key words Squamous cell carcinoma ; Head and neck region ; Prognosis ; Cox regressionSource: Springer Online Journal Archives 1860-2000Topics: MedicineDescription / Table of Contents: Abstract In individual patients with head and neck squamous cell carcinomas (HNSCC), established prognostic factors do not satisfactorily predict clinical outcome. For the first time we investigated a total of 100 HNSCC by Comparative Genomic Hybridization (CGH) to define chromosomal alterations that are associated with the patients prognosis. Patients were followed for at latest 4 but at least 2 years after surgery or until death. During this observation period twenty-nine of them died because of cancer disease. The Kaplan-Meier method was used plotting survival curves for every single chromosomal alteration as well as every clinico-pathological parameter. The curves were tested for significance by the log rank as well as the Breslow test. Significance of particular prognostic parameters was then evaluated by the Cox regression model. The overall survival time as well as the recurrence free survival time were significantly lower in patients who's tumors showed amplifications of the chromosomal region 11q13 (p=0.0008 for LR and p=0.0024 for B). The survival time of the patients was also lower if the carcinomas carried overrepresentations of chromosome 3q (p=0.0299 for LR and p=0.0546 for B). Multivariate analysis (Cox's proportional hazards model) revealed both alterations as most important independent prognostic factors in HNSCC. None of the conventional clinico-pathological parameters (pT-, pN-status, UICC stage, grading) achieved statistical significance in the multivariate model. These results suggest that in HNSCC the occurence of 11q13 amplification and 3q overrepresentation are highly significant independent prognostic markers and of better value than the established TNM and grading criteria.Notes: Zusammenfassung Die Prognose von Patienten mit Kopf-Hals-Karzinomen lässt sich anhand der etablierten Stagingparameter oft nur sehr ungenügend einschätzen. Auf der Suche nach neuen Markern, die den Tumorphänotyp genauer charakterisieren, wurden in der vorliegenden Studie 100 primäre Plattenepithelkarzinome des Kopf-Hals-Bereichs mit Hilfe der Comparativen Genomischen Hybridisierung (CGH) molekularzytogenetisch untersucht. Die detektierten genetischen Veränderungen (Deletionen oder DNA-Überrepräsentierungen bzw. Amplifikationen) wurden für jeden Chromosomarm aufgeschlüsselt und statistisch zusammen mit den klinisch-pathologischen Daten uni- und multivariat hinsichtlich ihrer prognostischen Aussagefähigkeit überprüft. Alle Patienten dieser Studie wurden primär operiert und in Abhängigkeit vom Tumorstadium adjuvant nachbestrahlt. In dem längstens 4-jährigen Beobachtungszeitraum waren von den 100 Patienten 29 am Tumorleiden verstorben. Die kumulierten Überlebensraten nach der Kaplan-Meier-Methode zeigten, dass sowohl die rezidiv- und metastasenfreie als auch die Gesamtüberlebenszeit signifikant kürzer ist bei Patienten, deren Tumoren eine Amplifikation der chromosomalen Region 11q13 und/oder eine DNA-Überrepräsentierung des Chromsomarms 3q aufweisen (p=0,0008 bzw. p=0,0299 im Log-Rank-Test und p=0,0024 bzw. p=0,0546 im Breslow-Test). Die Cox-Regression wurde für das rezidiv- und metastasenfreie Überleben und für das Gesamtüberleben jeweils mit den klinisch-pathologischen Parametern pT-, pN-Status, UICC-Stadium, Grading und den genetischen Markern 11q13-Amplifikation sowie 3q-Überrepräsentierung durchgeführt. Die Analyse ergab die genetischen Veränderungen im Bereich von 11q13 und 3q als die wichtigsten Marker für die Einschätzung des Überlebens, während die klinisch-pathologische Tumorklassifikation ohne prognostischen Einfluss war (bei Signifikanzniveau p〈0,05). Das Ergebnis unserer Studie eröffnet die Möglichkeit eines “genetischen Tumorgradings”, das als Zusatzinformation einer verbesserten Prognoseeinschätzung bei Kopf-Hals-Karzinomen dient.Type of Medium: Electronic ResourceURL: -
19Articles: DFG German National LicensesStaff View
ISSN: 1432-0517Keywords: Key words Uncertainty budget ; Uncertainty evaluation ; Uncertainty component ; Traceability ; Reference standardsSource: Springer Online Journal Archives 1860-2000Topics: Chemistry and PharmacologyNotes: Abstract The uncertainty of the Kjeldahl method for determination of nitrogen in insulin was evaluated according the procedure described in the Guide to the Expression of Uncertainty in Measurement. The relative standard uncertainty of the method was found to be 0.19%, compared to the relative intermediate precision experimentally found to be 0.085%. The uncertainty components were organized in Tables, which allowed an easy overview and evaluation. The largest contribution to the uncertainty came from volumetric equipment. Systematic uncertainty budgets such as the design presented here facilitate the uncertainty evaluation process and makes it easier to compare uncertainty evaluations performed by different analysts.Type of Medium: Electronic ResourceURL: -
20Articles: DFG German National LicensesStaff View
ISSN: 1420-9071Source: Springer Online Journal Archives 1860-2000Topics: BiologyMedicineNotes: Zusammenfassung Eswurde in elektrophysiologischen Versuchen gezeigt, dass bei dem Affen (Macaca mulatta) die spinalen Motoneuronen von den kortikospinalen Neuronen monosynaptisch aktiviert werden können. Einige Eigenschaften der monosynaptischen Aktivität wurden beschrieben und einige klinische Aspekte aufgezeigt.Type of Medium: Electronic ResourceURL: