Search Results - (Author, Cooperation:H. Suh)

2015-07-15

American Association for the Advancement of Science (AAAS)

0036-8075

1095-9203

Biology

Chemistry and Pharmacology

Computer Science

Medicine

Natural Sciences in General

Physics

2018-11-14

The American Society for Pharmacology and Experimental Therapeutics (ASPET)

0026-895X

1521-0111

Chemistry and Pharmacology

Medicine

2011-09-09

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Animals ; BRCA1 Protein/deficiency/genetics/*metabolism ; Breast/cytology ; Breast Neoplasms/*genetics/pathology ; Cell Line, Tumor ; Cells, Cultured ; DNA, Satellite/genetics ; Epithelial Cells/metabolism ; Female ; Gene Expression Regulation, Neoplastic ; *Gene Silencing ; Genes, BRCA1/*physiology ; Genomic Instability/genetics ; HeLa Cells ; Heterochromatin/*genetics/*metabolism ; Histones/metabolism ; Humans ; Mice ; Ovarian Neoplasms/genetics ; RNA, Messenger/genetics ; Transcription, Genetic/genetics ; Ubiquitin-Protein Ligases/metabolism ; Ubiquitinated Proteins/metabolism ; Ubiquitination

2018-03-16

American Physical Society (APS)

1098-0121

1095-3795

Physics

Magnetism

1471-4159

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Abstract: The contribution of an ω-conotoxin GVIA (ωCgtx)-sensitive Ca2+ influx pathway to the effects of angiotensin II (AII) receptor activation was examined in bovine adrenal medullary (BAM) cells. Pretreatment of BAM cells with 10–6MωCgtx blocked stimulation of exocytosis by the degradation-resistant analogue, sarcosine1–angiotensin II (S1-AII). In contrast, ωCgtx had no effect on basal secretion, nor did it inhibit [3H]norepinephrine and [32P]ATP release in response to bradykinin, another phospholipase C-linked receptor agonist. Similarly, ωCgtx pretreatment inhibited the stimulation of 45Ca2+ uptake by S1-AII, but did not affect the response to bradykinin. This selective inhibition did not appear to be due to blockade of AII receptors by ωCgtx, as the accumulation of 3H-labeled inositol phosphates in response to S1-AII was not inhibited. The peak S1-AII-stimulated increase in the intracellular free Ca2+ concentration (Cai) in fura 2-loaded BAM cells also was not significantly reduced by ωCgtx (or by stimulating in nominally Ca2+-free buffer), indicating that this response is dependent on intracellular Ca2+ pools. However, a small ωCgtx-sensitive Cai response was detected after depletion of intracellular Ca2+ pools with ionomycin. This study shows that AII receptors, but not bradykinin receptors, are linked to an ωCgtx-sensitive Ca2+ influx pathway in BAM cells.

Electronic Resource

1471-4159

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Abstract: Expression of the carboxyl-terminal fragment (CT) of the β-amyloid precursor protein (APP) in transgenic animals has been linked with neurotoxicity. However, it remains to be clarified whether the neurotoxicity is caused by β-amyloid proteins (Aβs) derived from CT or by CT itself. To study the in vivo neurotoxicity of CT, mice were given a single intracerebroventricular injection of a recombinant 105-amino acid CT (CT105; 68.5–685 pmol, intracerebroventricularly), and changes in behavior and in brain histology were examined. Animals given CT105 (410 or 685 pmol, intracerebroventricularly) showed a dose-dependent impairment in the passive avoidance performance, whereas boiled CT105 had no effect. CT105 (685 pmol, intracerebroventricularly) induced reactive gliosis in neocortex and hippocampus and neurodegeneration in neocortex. These results indicate that centrally administered CT105 induces behavioral impairment and neuropathologic changes, suggesting a direct toxic effect of CT105 per se.

Electronic Resource

1365-2230

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Electronic Resource

1077-3118

AIP Digital Archive

Physics

A method of frequency and power stabilization for an internal-mirror He-Ne laser using the secondary beat frequency between the three longitudinal modes is presented. Frequency and power fluctuation was ±1 MHz (2 parts in 109) and ±0.10% over 10 000 s, respectively. The square-root Allan variance between the laser stabilized in this study and the iodine stabilized He-Ne laser was 5×10−11 at average time of τ=1 s. Furthermore, we could obtain the output power of 2 mW in a single longitudinal mode.

Electronic Resource

1089-7623

AIP Digital Archive

Physics

Electrical Engineering, Measurement and Control Technology

Pohang Accelerator Laboratory (PAL) is developing an Elliptically Polarized Undulator (EPU) to produce polarized synchrotron radiation at Pohang Light Source (PLS). The EPU6 is an Apple-II type elliptical undulator, which changes the polarization of the field by translating the quadrant arrays. The PLS EPU6 features a period of 6 cm, a minimum gap of 19 mm, 25 full field periods, a maximum vertical flux density of 0.69 T, a maximum horizontal flux density of 0.46 T, and 1575 mm total magnetic structure length. The EPU6 is optimized for highly polarized synchrotron radiation from 117 to 1500 eV at 2.5 GeV electron energy. Using correction coils to compensate the orbit drift in the undulator increased the estimated photon flux by 15% compared to the case without the compensation. © 2002 American Institute of Physics.

Electronic Resource

1365-2230

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Electronic Resource

0003-9861

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Chemistry and Pharmacology

Physics

Electronic Resource

0040-4039

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Chemistry and Pharmacology

Electronic Resource

1432-1912

Key words GABA receptors ; Opoids ; Antinoiception

Springer Online Journal Archives 1860-2000

Medicine

Abstract  The present study was designed to investigate the modulatory effects of stimulation of GABAA and GABAB receptors at supraspinal sites on antinociception induced by supraspinally administered μ-, ɛ-, δ-, and κ-opioid receptor agonists. The effects of the GABAA and GABAB receptor agonists, muscimol and baclofen respectively, on the antinociception induced by morphine (a μ-receptor agonist), β-endorphin (an ɛ-receptor agonist), D-Pen2,5-enkephalin (DPDPE,a δ-receptor agonist) and U50,488H ({trans-3,4-dichloroN-methyl-N-[2-(1-pyrrolidinyl)cyclohexyl] benzeocetamide}; a κ-receptor agonist) injected intracerebroventricularly (i.c.v.) were studied. The antinociception was assayed using the tail-flick and hot-plate tests. Muscimol at doses of 25–200 ng, administered i.c.v. alone did not affect the latencies of tail-flick and hot-plate thresholds, but attenuated dose-dependently the inhibition of the tail-flick and hot-plate responses induced by i.c.v. administered morphine (2 μg), β-endorphin (1 μg), DPDPE (10 μg), and U50,488H (60 μg). Baclofen (1.25–10 ng) administered i.c.v. alone did not affect the latencies of the tail-flick and hot-plate responses, but attenuated dose-dependently the inhibition of the tail-flick and hot-plate responses induced by β-endorphin and U50,488H, without affecting morphine- or DPDPE-induced responses. Our results indicate that activation of GABAA receptors at the supraspinal sites by i.c.v. injection of muscimol antagonizes antinociception induced by supraspinally administered μ-, ɛ-, δ-, and κ-opioid receptor agonists. On the other hand, activation of GABAB receptors at supraspinal sites by i.c.v. baclofen antagonizes antinociception induced by i.c.v. administered ɛ- and κ-opioid agonists, but not μ- or δ-opioid agonists.

Electronic Resource

1432-1793

Springer Online Journal Archives 1860-2000

Biology

Abstract Foregut contents of calyptopes (the first feeding stage) ofEuphausia pacifica Hansen, collected from the Yellow Sea in June 1989, were examined using scanning electron microscopy with the dry-fracturing method. There was no clear evidence of phytoplankton ingestion by calyptopes. The foregut contents consisted entirely of amorphous organic detritus, crustacean remains and inorganic particles. The inorganic particles adhered to amorphous organic detritus, and bacterial populations were found on peritrophic membranes of fecal pellets which enveloped crustacean remains. These materials seemed to have originated from aggregates of organic and inorganic detritus. It is quite possible that the detrital aggregates are utilized extensively as food by calyptopes ofE. pacifica in the Yellow Sea.

Electronic Resource

1432-1793

Springer Online Journal Archives 1860-2000

Biology

Abstract The complete larval development of Euphausia pacifica in the Yellow Sea is described and the stages are compared with larvae of E. nana. Euphausiid larvae examined in the present study were collected at 30 stations in the Yellow Sea in 1989. During the stages of Calyptopis III to Furcilia II, E. pacifica larvae are smaller than E. nana larvae but they are larger from Furcilia III onward. There are geographical variations in body size of Calyptopis III among the southern California waters, the eastern Japan waters, the Japan Sea, and the Yellow Sea; body size is smallest in the Yellow Sea and Japan Sea, while it is largest in southern California waters and intermediate in eastern Japan waters. Segmentation of antennal endopods was observed in the 5″6 to 5″1 furcilia forms in the Yellow Sea population, suggesting that this characteristic is not as reliable for identification of furcilia stages as the pleopods and terminal telson spines. Thus, we propose here that there are six furcilia stages of E. pacifica, instead of the seven previously reported by Boden (1950).

Electronic Resource

1573-4927

disc electrophoresis ; sorghum trisomics ; SDS gel electrophoresis ; isoelectric focusing

Springer Online Journal Archives 1860-2000

Biology

Chemistry and Pharmacology

Abstract To determine protein differences of grain sorghum disomics and trisomics, we analyzed leaf extracts from six trisomics and a disomic control by disc gel, gel isoelectric focusing, and SDS gel electrophoresis. Based on the number and position of protein bands revealed by Commassie blue staining, the disomic control could be differentiated from the trisomics, and trisomics could be shown to differ among themselves in most cases. SDS gels revealed the most protein bands, followed by isoelectric focusing and disc gel. However, disc gel electrophoresis was the simplest technique of the three and was just as effective in identifying trisomics and differentiating trisomics from the disomic control.

Electronic Resource

1432-1793

Springer Online Journal Archives 1860-2000

Biology

Abstract SEM observation revealed the detailed morphology of the gastric mill in ten species of euphausiids, Bentheuphausia amblyops, Thysanopoda acutifrons, Meganyctiphanes norvegica, Pseudeuphausia latifrons, Euphausia superba, Tessarabrachion oculatum, Thysanoessa longipes, Nematoscelis microps, Nematobrachion boopis, and Stylocheiron maximum. The well-developed gastric mill of euphausiids consists of a pair of cluster spines and a pair of lateral teeth displaying great diversity in morphology. It is suggested that there are three categories of gastric mill and filterpress (gland filter) within euphausiids. One is the well-developed gastric mill with filter-press of B. amblyops; a second is the well-developed gastric mill of M. norvegica, T. oculatum, E. superba, P. latifrons and T. longipes; and the third is the gastric mill without lateral teeth of T. acutifrons, N. microps, N. boopis and S. maximum.

Electronic Resource

1432-2242

Key words Weedy rice ; Oryza sativa ; Classification ; Origin of weedy rice ; Isozyme ; Randomly amplified polymorphic DNA

Springer Online Journal Archives 1860-2000

Biology

Abstract  Weedy rice (Oryza sativa L.) is an important resource for breeding and for studying the evolution of rice. The present study was carried out to identify the genetic basis of the weedy rices distributed in various countries of the world. One hundred and fifty two strains of weedy rice collected from Bangladesh, Brazil, Bhutan, China, India, Japan, Korea, Nepal, Thailand and the USA were tested for variations in six morpho-physiological characteristics and in 14 isozyme loci. Twenty six weedy strains selected from the above materials were assayed for the Est-10 locus, six RAPD loci of the nuclear genome, and one chloroplast locus. From the results of multivariate analysis based on the morpho-physiological characteristics and the isozymes, weedy rice strains were classified into indica and japonica types, and each type was further divided into forms resembling cultivated and wild rice. Thus, four groups designated as I, II, III and IV were identified. Weedy strains of group I (indica-type similar to cultivars) were distributed mostly in temperate countries, group II (indica-type similar to wild rice) in tropical countries, group III (japonica-type similar to cultivars) in Bhutan and Korea, group IV ( japonica-type similar to wild rice) in China and Korea. In group I, classified as indica, several strains showed japonica-specific RAPD markers, while some others had japonica cytoplasm with indica-specific RAPD markers in a heterozygous state at several loci. One weedy strain belonging to group II showed a wild rice-specific allele at the Est-10 locus. However, in groups III and IV, no variation was ound either for the markers on Est-10 or for the RAPD loci tested. Judging from this study, weedy rice of group I might have originated at least partly from gene flow between indica and japonica, whereas that of group II most probably originated from gene flow between wild and cultivated indica rice. Weedy rice of group III is thought to have originated from old rice cultivars which had reverted to a weedy form, and that of group IV from gene flow between japonica cultivars and wild rice having japonica backgrounds.

Electronic Resource

1434-4726

Key words Surgical wound infection ; Otologic ; surgery ; Methicillin-resistant Staphylococcus aureus ; Ribotyping ; EcoR1

Springer Online Journal Archives 1860-2000

Medicine

Abstract The incidence of methicillin-resistant Staphylococcus aureus (MRSA) infections after middle ear surgery has recently increased at our hospital. Most of these infections were thought to be hospital-acquired when medical personnel in contact with an MRSA-infected patient may have inadvertently transmitted the pathogen to other patients. To prevent further transmission it is essential that such sources of MRSA infection and transmission routes be selected out and eradicated. Therefore, it is necessary to determine whether the strains of MRSA isolated from infected patients are identical to those obtained from medical personnel in order to prove a reciprocal transmission of organisms between medical personnel and patients. Surveillance bacterial cultures from the anterior nares and hands of medical personnel working in the Department of Otolaryngology, Korea University Guro Hospital, were performed at two different time points: 6 December 1994 and 17 June 1996. Ribotyping with Southern blot technique was used to compare 12 MRSA strains from medical carriers with 60 strains identified from the otorrhea of MRSA-infected patients undergoing middle ear surgery. As result, six different MRSA strains were identified (types I, II, III, IV, V and VI) from ribotyping with EcoR1. One distinct subtype, type I strain, was the most frequently identified strain in both medical carriers and patients. Results also showed that 6 MRSA isolates from 10 medical carriers and 20 from 30 patients contained type I ribotype at first culture. Two medical carriers’ isolates and 13 isolates from 30 patients shared the same type I strain at the second surveillance culture. In all, 41 out of 72 MRSA strains (56.9%) shared an identical ribotype pattern. Postoperative MRSA infection rates after treatment of medical carriers and the application of rigorous preventive procedures decreased from 11.9 to 5.7% after first culture and 9.0 to 7.7% following second cultures. These findings confirm that MRSA transmission can occur between medical personnel and patients and that effective preventive measures can reduce the postoperative infection rate.

Electronic Resource