Search Results - (Author, Cooperation:H. Q. Ling)
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1H. Q. Ling ; S. Zhao ; D. Liu ; J. Wang ; H. Sun ; C. Zhang ; H. Fan ; D. Li ; L. Dong ; Y. Tao ; C. Gao ; H. Wu ; Y. Li ; Y. Cui ; X. Guo ; S. Zheng ; B. Wang ; K. Yu ; Q. Liang ; W. Yang ; X. Lou ; J. Chen ; M. Feng ; J. Jian ; X. Zhang ; G. Luo ; Y. Jiang ; J. Liu ; Z. Wang ; Y. Sha ; B. Zhang ; D. Tang ; Q. Shen ; P. Xue ; S. Zou ; X. Wang ; X. Liu ; F. Wang ; Y. Yang ; X. An ; Z. Dong ; K. Zhang ; M. C. Luo ; J. Dvorak ; Y. Tong ; H. Yang ; Z. Li ; D. Wang ; A. Zhang
Nature Publishing Group (NPG)
Published 2013Staff ViewPublication Date: 2013-03-29Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Base Sequence ; Brachypodium/genetics ; Crops, Agricultural/classification/genetics ; Diploidy ; Genetic Markers/genetics ; Genome, Plant/*genetics ; Molecular Sequence Data ; Oryza/genetics ; Phylogeny ; Sorghum/genetics ; Synteny/genetics ; Triticum/classification/*genetics ; Zea mays/geneticsPublished by: -
2Staff View
ISSN: 1439-0523Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: Protoplasts of eight wild Linum species and 14 genotypes of flax, isolated from different parts of seedlings and from shoot apices, were regenerated to callus in medium V-KM (Binding and Nehls 1977). Plant regeneration was obtained in L. alpinum, L. amurense, L. hologynum, L. perenne, L. salsoloides and eight genotypes of L. usitatissimum on agar media B5 (Gamborg et al. 1968), B5C (Binding et al, 1981), MS (Murashige and Skoog 1962) and MSC (MS with 5% coconut endosperm) containing 2.5μM 6-BA. An adventitious embryo was regenerated in L. alpinum. Callus differentiation like sponge parenchyma, was observed in protoplast-derived tissues of L. usitatissimum. Shoots organized roots under slightly divergent conditions in the different species.Type of Medium: Electronic ResourceURL: -
3Staff View
ISSN: 1432-203XKeywords: Key wordsLycopersicon esculentum ; Agrobacterium tumefaciens ; Transformation ; Cefotaxime ; Ticarcillin/potassium clavulanateSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract Ticarcillin/potassium clavulanate is a very effective combination of antibiotics to eliminate Agrobacterium tumefaciens during tomato transformation. It shows no toxicity to tomato tissues at a concentration of 150 mg/l and significantly promotes callus formation and shoot regeneration. The transformation frequency was raised more than 40% in comparison to cefotaxime. Cefotaxime itself did not inhibit callus growth in culture medium, but it clearly decreased shoot differentiation. Together with kanamycin, cefotaxime shows a strong negative effect on callus growth, shoot regeneration and transformation efficiency. Unlike the widely used carbenicillin and cefotaxime, ticarcillin/potassium clavulanate is light stable and resistant to inactivation by β-lactamase. Furthermore, ticarcillin/potassium clavulanate is more economical than carbenicillin and cefotaxime. In conclusion, ticarcillin/potassium clavulanate is a very good alternative to eliminate Agrobacterium tumefaciens in plant transformation and has the potential to be widely used for plants which are sensitive to carbenicillin and cefotaxime.Type of Medium: Electronic ResourceURL: