Search Results - (Author, Cooperation:H. Nakajima)

2014-11-07

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

2011-10-04

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Antarctic Regions ; Arctic Regions ; Atmosphere/*chemistry ; Chlorine/chemistry ; *Environmental Monitoring ; History, 20th Century ; History, 21st Century ; Ozone/*analysis/chemistry/history ; Seasons ; Time Factors

2018-01-07

BMJ Publishing

2044-6055

Medicine

Public health, Open access, Public health, Epidemiology

2018-08-02

The Company of Biologists

0950-1991

1477-9129

Biology

Cardiovascular development and regeneration

2018-06-20

Institute of Physics (IOP)

1757-8981

1757-899X

Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics

2018-10-02

Rockefeller University Press

0022-1007

1540-9538

Medicine

1524-475X

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Aim: The movements of the cell population are different between 2-D culture and 3-D culture. From the observation of morphology of skeletal muscle cell 3-D culture, We expect that skeletal muscle cells differentiation is accelerate in the collagen gel 3-D culture, and the proliferation is suppressed. The purpose of this study is to investigate the difference between 2-D culture and 3-D culture of C2C12 cells. Methods: C2C12 skeletal muscle cells are incubated following three difference conditions for 48 hours, plastic dish 2-D culture, collagen coated dish 2-D culture and collagen gel 3-D culture. The culture medium is Dulbecco's modified Eagle medium containing 10% fetal bovine serum and 1% penicillin/streptomycin. Collagens are removed by collagenase treatment and cells are homogenized. After centrifugation the top clear layer is used for CPK assay and protein development analysis by Western blotting Results: After 48 hour incubation, we observed cell morphology by a phase contract microscope. Cell fusion was observed in collagen gel 3-D culture. The fusion cells have many nucleus in the cytoplasm called synthetium. But in plastic dish 2-D culture and in collagen coated dish 2-D culture synthetiums were not observed and cells were mononuclear and monolayer. Cell prolieration was suppressed in collagen gel 3-D culture. CPK activity was five times activated in collagen gel 3-D culture than in plastic dish 2-D culture. Conclusions: We suggest skeletal muscle cells C2C12 are activate differentiation by collagen gel 3-D culture.

Electronic Resource

1365-2133

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

We describe familial cases of cutaneous infection caused by Mycobacterium avium. A 45-year-old father, his 14-year-old son and 11-year-old daughter, among five persons in a family, presented with a 2-month history of inflammatory subcutaneous nodules and ulcerations. Histology of skin biopsy specimens showed granulomatous inflammation, and mycobacterial colonies isolated from the skin of each patient were identified as M. avium by DNA hybridization analysis. The patients were all treated successfully with combined drug therapy consisting of rifampicin, isoniazid and clarithromycin. Their lesions were purely cutaneous M. avium infection, without any visceral involvement. Neither systemic disease nor immunological impairment was detected in the family. However, they all used a circulating, constantly heated bath water system. The bath water was continuously heated to about 40 °C without changing the water for a few months, and M. avium was isolated from the filter of the bath tub heating unit. It is considered that this unusual familial cluster of cutaneous M. avium infection in healthy persons may have resulted from the use of contaminated bath water.

Electronic Resource

1365-2133

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

The expression of tumour necrosis factor (TNF)-α in leprosy skin lesions was examined before and during successful treatment in a patient with borderline lepromatous leprosy. Before treatment, immunohistochemical staining of a skin biopsy specimen showed diffuse TNF-α deposits in granulomas and significant TNF-α deposits on infiltrated mononuclear cells. After 1 year's treatment, the skin lesions exhibited a reduction in granulomas, and a concomitant reduction in deposits of TNF-α.Furthermore, the level of expression of TNF-α messenger RNA. as examined using a reverse transcriptase-polymerase chain reaction method, was reduced markedly after treatment. These findings provide evidence for a correlation between the expression of TNF-α and disease activity suggesting that TNF-α is a useful prognostic indicator for inflammation in leprosy.

Electronic Resource

1077-3118

AIP Digital Archive

Physics

An FeAu ordered alloy has been fabricated artificially by depositing alternately monatomic layers of Fe and Au. Neither intermediate phase nor intermetallic compound is known for the Fe–Au system in the equilibrium state. Nevertheless, the x-ray diffraction for prepared FeAu films shows definite superlattice lines corresponding to the L10 ordered structure, and it is metastable at room temperature. The obtained FeAu ordered alloy is ferromagnetic, and it has the Fe moment of 2.5±0.3 μB, a considerably high Curie temperature over room temperature, and a large uniaxial magnetic anisotropy perpendicular to Fe and Au atomic planes. © 1995 American Institute of Physics.

Electronic Resource

1077-3118

AIP Digital Archive

Physics

Interdiffusion and interfacial reaction between a sputter-deposited YBa2Cu3Ox thin film and substrates (MgO, sapphire, quartz, and Si) have been investigated by 2.7 MeV 4He+ Rutherford backscattering spectrometry. The diffusivities of Cu in the substrates and of the substrate elements (Mg, Al, and Si) in the Y-Ba-Cu-O thin films have been determined over the annealing temperature range from 876 to 1226 K in an oxygen atmosphere. Among three metallic elements of Y, Ba, and Cu, Cu diffuses the fastest into the substrates. Copper silicide is observed in the interfacial region between the film and the Si substrate. Among these substrates, MgO is suggested to be the most stable on annealing.

Electronic Resource

1089-7550

AIP Digital Archive

Physics

Multilayered Mo/Si thin films have been prepared by dual rf-magnetron sputtering technique, and the superconducting transition temperature Tc and the upper critical field Hc2 have been examined by resistance measurements. X-ray diffractometry shows that for the modulation wavelength λ shorter than 3 nm both Mo and Si sublayers are amorphous, while for λ longer than 3 nm, crystalline bcc structures appear in Mo sublayers. The λ dependence of Tc of the multilayer films with equal sublayer thickness shows that Tc takes a maximum value of 6.1 K for λ=2.3 nm and approaches the Tc value of amorphous Mo56Si44 in the small λ limit. The superconductivity of the multilayers has been confirmed to result from amorphous MoSi phase formed in the interfacial region of the sublayers. The thickness of the superconducting amorphous layer is estimated to be about 3 nm from the λ dependence of Tc and from the result of the anisotropy of Hc2 . Keeping the Mo sublayer thickness less than 2.4 nm to suppress the appearance of the crystalline Mo and increasing the Si sublayer thickness to reduce the interlayer Josephson coupling, an ideal quasi-two-dimensional superconductor with dHc2(parallel) /dT as high as 180 kOe/K has been realized.

Electronic Resource

1471-4159

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Abstract— The marked cerebellar hypoplasia found in the homozygous (jj) Gunn rat with hereditary unconjugated hyperbilirubinaemia may provide an explanation of bilirubin neurotoxicity in vivo. In the jj Gunn rat. Purkinje cells were nearly selectively affected in the cerebellar cortex, and the cerebellar weight showed no increase after 10 days of age. The development-dependency of the cerebellar lesion was supported by the observation that the cerebellar lobuli which developed earlier were less affected. Brain bilirubin in the developing jj Gunn rat was determined by a spectrophotometric method, and was found to be extremely low (1–3 μg). The level of brain bilirubin decreased after birth, and showed little correlation with the level of bilirubin free of albumin which correlated clearly with total serum bilirubin level even in the neonate. These findings suggest that there is an affinity of brain tissue for bilirubin associated with the blood-brain barrier to bilirubin. No significant difference was found between the levels of bilirubin in the cerebellum and those of other brain regions in jj Gunn rat.These results seem to imply that the development-dependency of cerebellar hypoplasia in the jj rat may be due to the characteristic nature of rat cerebellar development, i.e. the postnatal neurogenesis. and not to changes in brain bilirubin levels. In the jj Gunn rat. cerebellar cell proliferation appears to be in some way affected by bilirubin during cerebellar development.

Electronic Resource

1077-3118

AIP Digital Archive

Physics

InP/InGaAs double-heterostructure bipolar transistors (DHBTs), incorporating a new collector structure featuring "pn pair doping'' in the heterointerface vicinity, have been fabricated using a low-pressure metalorganic chemical vapor deposition (MOCVD) method. These transistors provide high collector current densities over 1×105 A/cm2, indicating the successful suppression of current blocking. S-parameter measurements determine the high current gain cutoff frequencies of 130 GHz. These values favorably compare with those of conventional InGaAs-collector HBTs fabricated for comparison, suggesting that the InP collectors have excellent electron transport properties.

Electronic Resource

1077-3118

AIP Digital Archive

Physics

We report the successful growth of high-quality InP/InGaAs double-heterojunction bipolar transistors with heavily Zn-doped base layers (p=4×1019 cm−3) using metalorganic vapor phase epitaxy. Interrupting the growth for 30 min after growing a heavily Si-doped subcollector (n=2×1019 cm−3) is shown to eliminate the excessive nonequilibrium group III interstitials that enhance abnormal Zn diffusivity in the subsequently grown base region. Gummel plots of the fabricated microwave transistors show that they have ideal turn-on characteristics in spite of having only 5-nm-thick undoped spacer layers inserted between the emitter and base. The transistors obtain a maximum oscillation frequency fmax of 178 GHz and a current gain cutoff frequency fT of 126 GHz.

Electronic Resource

1077-3118

AIP Digital Archive

Physics

Intersubband light absorption measurements have been performed on a series of GaAs/AlAs quantum wells with heavy delta doping of Si atoms in the range 9.0×1011–6.5×1012 cm−2. The increase of intersubband transition energy by as much as 38 meV has been observed, and attributed to the deepening of the V-shaped potential by the Si layer and to the depolarization effects. © 1998 American Institute of Physics.

Electronic Resource

1365-2230

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Eleven cases of cutaneous B-cell lymphoma (CBCL) were studied. The ages at presentation ranged from 34 to 79 years (mean = 59·9 years). Six patients were female and five male. Five of the 11 patients had a solitary tumour and the other six had multiple tumours at initial presentation. According to Burg's classification, six cases were at stage I, two stage II, two stage III and one was at stage IV at initial presentation. Abnormalities in laboratory data were rare, except for serum lactic dehydrogenase values. Epidermotropism was not detected, and the area mainly affected by neoplastic cells was the reticular dermis (seven cases) and suhcutis (four cases). Biopsy-specimens from the patients analysed by immunohisiochemical techniques on paraffin or cryostat sections showed CD20 and/or CD22 positivity. Biopsy specimens from two patients which showed CD 10 positivity were diffuse large cell types by the working formulation and presented as pre-B-cell lymphoma. At least two groups of CBCL were demonstrable on the basis of prognosis. One was a benign low-grade lymphoma presenting with solitary tumours, mature B-cell markers and intermediate-grade pathology, and the other was a high-grade lymphoma with multiple tumours, pre-B-cell or mature B-cell markers and a poor prognosis.

Electronic Resource

1365-2230

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Intravascular papillary endothelial hyperplasia (IPEH) was first described by Masson.1 It is characterized by the development of endothelial-lined papillary projections in a vascular lumen. In Japan the disease was first reported by Tanimura et al.2 in 1976, and since then over 30 cases have been described.In this reort we describe a case of IPEH together with the review of these Japanese cases.

Electronic Resource

1365-2230

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

We report a case of focal dermal hypoplasia, or Goltz syndrome in a 13-year-old girl. The patient presented with a variety of cutaneous defects, including atrophy-like depressions, striations, a verrucous papilloma, and lipomas. Histopathological examination showed deposits of fat cells or adipose tissue in the dermis, which were subepidermal, mid-dermal, perivascular, or involved the whole dermis. These findings indicate that the adipose tissue in the dermis is a result of dermal dysplasia and not hypoplasia.

Electronic Resource

1439-0264

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

We prepared a map of the cleavage lines for beagle dogs, as a guideline for use of cleavage lines in dermatoplasty. The cleavage lines at the head resembled the orientation of the underlying muscles. Although the cleavage lines in the trunk were perpendicular to the body axis, those in the thoraco-abdominal region were parallel to the body axis. The cleavage lines at the limbs were parallel to the long axis of the limb on the cranial surface, but were perpendicular to the long axis of the limb on the lateral and caudal surfaces. Also, we recorded in detail the cleavage lines in the distal regions of the limbs.

Electronic Resource