Search Results - (Author, Cooperation:H. Mochizuki)
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1Latest Papers from Table of Contents or Articles in PressM. Maekawa ; K. Yamaguchi ; T. Nakamura ; R. Shibukawa ; I. Kodanaka ; T. Ichisaka ; Y. Kawamura ; H. Mochizuki ; N. Goshima ; S. Yamanaka
Nature Publishing Group (NPG)
Published 2011Staff ViewPublication Date: 2011-06-10Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Animals ; Cell Line ; Cells, Cultured ; *Cellular Reprogramming ; DNA-Binding Proteins/*metabolism ; Embryonic Stem Cells/cytology/metabolism ; Epithelial-Mesenchymal Transition ; Female ; Fibroblasts/*cytology/*metabolism ; Gene Expression Profiling ; Genes, myc/genetics ; Hepatocyte Nuclear Factor 3-beta/metabolism ; Homeodomain Proteins/metabolism ; Humans ; Induced Pluripotent Stem Cells/*cytology/*metabolism ; Mice ; Nuclear Transfer Techniques ; Oligonucleotide Array Sequence Analysis ; Protein Binding ; RNA-Binding Proteins/metabolism ; Receptors, Estrogen/metabolism ; Transcription Factors/*metabolismPublished by: -
2Articles: DFG German National LicensesSuzuki, A. ; Obi, K. ; Urabe, T. ; Hayakawa, H. ; Yamada, M. ; Kaneko, S. ; Onodera, M. ; Mizuno, Y. ; Mochizuki, H.
Oxford, UK : Blackwell Science Ltd
Published 2002Staff ViewISSN: 1471-4159Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Neuronal progenitor cells (NPC) are particularly suited as the target population for genetic and cellular therapy of neurological disorders such as Parkinson's disease or stroke. However, genetic modification of these cells using retroviral vectors remains a great challenge because of the low transduction rate and the need for fetal calf serum (FCS) during the transduction process that induces the cell differentiation to mature neurons. To overcome these problems, we developed a new retrovirus production system in which the simplified retroviral vector GCDNsap engineered to be resistant to denovo methylation was packaged in the vesicular stomatitis virus G protein (VSV-G), concentrated by centrifugation, and resuspended in serum-free medium (StemPro-34 SFM). In transduction experiments using enhanced green fluorescent protein (EGFP) as a marker, the concentrated FCS-free virus supernatant infected NPC at a high rate, while maintaining the ability of these cells to self-renew and differentiate in vitro. When such cells were grafted into mouse brains, EGFP-expressing NPC were detected in the region around the injection site at 8 weeks post transplantation. These findings suggest that the gene transfer system described here may provide a useful tool to genetically modify NPC for treatments of neurological disorders.Type of Medium: Electronic ResourceURL: -
3Articles: DFG German National LicensesTOKUYAMA, K. ; ARAKAWA, H. ; MOCHIZUKI, H. ; MORIKAWA, A.
Oxford, UK : Blackwell Publishing Ltd
Published 1995Staff ViewISSN: 1365-2222Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Beta-2 adrenoceptor agonists are widely used as bronchodilators in the treatment of asthma mainly via inhalation. In the present study, we evaluated the ability of inhaled procaterol, a β2 adrenoceptor agonist, to reduce eicosanoid-induced airway micro-vascular leakage, and compared the ability with its inhibitory effect against bron-choconstriction. Tracheostomized guinea-pigs were given aerosolized procaterol (10 or 100 μg/ml) for 10min under spontaneous breathing. Immediately after the end of inhalation, the animals were mechanically ventilated. Fourteen minutes after the end of inhalation, Evans blue dye (20mg/kg) was given i.v. One minute later, 2nmol/kg leukotriene D4 (LTD4), 50 nmol/kg U-46619, a thromboxane A2 mimetic, or vehicle was administered i.v. LTD4- or U-46619-induced increase in lung resistance was measured for 6 min. After removing the lungs, the amount of extravasated Evans Blue due in the lower airways was examined as an index of microvascular leakage. Inhaled procaterol significantly attenuated increases in both lung resistance and Evans Blue dye extravasation caused by these eicosanoids. The degree of inhibition was almost complete for lung resistance (approximately 90%), but it was only partial (range 18.5–61.2%) for the dye extravasation. No significant changes in mean systemic blood pressure and in heart rate was observed after an inhalation of 10μg/ml procaterol. These results suggest that inhaled β2 adrenoceptor agonists may reduce airway microvascular leakage caused by inflammatory mediators such as eicosanoids without affecting systemic circulation. However, these agonists may attenuate airway microvascular leakage only partially even in doses which can inhibit bronchoconstric-tion almost completely.Type of Medium: Electronic ResourceURL: -
4Articles: DFG German National LicensesTamura, K. ; Arakawa, H. ; Suzuki, M. ; Kobayashi, Y. ; Mochizuki, H. ; Kato, M. ; Tokuyama, K. ; Morikawa, A.
Oxford, UK : Blackwell Science, Ltd
Published 2001Staff ViewISSN: 1365-2222Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Background T helper-type 2 cytokines, such as interleukin-4 (IL-4) and IL-13, may play a central role in allergic diseases. The protein known as ‘signal transducers and activators of transcription 6’ (STAT-6) is a key transcription factor involved in both IL-4- and IL-13-mediated biological responses.Objective The objective of this study was to evaluate the possible role of the STAT-6 gene in modulating atopy in the Japanese population.Methods We screened all 23 exons of the STAT-6 gene from 10 subjects for mutations by direct polymerase chain reaction (PCR) sequencing. The STAT-6 gene polymorphisms were genotyped by PCR fragment length polymorphism analysis and PCR-SSCP analysis. The IL-4 receptor Q576R polymorphism was also examined by PCR-SSCP analysis.Results We found a novel dinucleotide repeat polymorphism in the first exon of the STAT-6 gene. The genotypes were classified into four groups according to the number of GT repeats present, from 13 to 16. The frequency of the A1 allele (326 bp, containing 13 repeats of GT) was higher in children with allergic diseases (bronchial asthma, atopic dermatitis and/or food-related anaphylaxis) than controls, although this was not statistically significant (P = 0.0158). In addition, a strong association between the A1 and A3 allele (containing 15 repeats of GT) heterozygote and allergic diseases was identified (P = 0.0002). However, the levels of IgE were not related to the GT repeat polymorphism in the allergic subjects. The GT repeat polymorphism was not associated with the polymorphism in the IL-4 receptorachain gene (Q576R) and there was no association between the G2964A variant and allergic diseases.Conclusion This suggests that genetic variation in the STAT-6 gene may be associated with predisposition to allergic diseases.Type of Medium: Electronic ResourceURL: -
5Articles: DFG German National LicensesTokuyama, K. ; Takei, K. ; Arakawa, H. ; Kato, M. ; Shimizu, T. ; Hoshino, M. ; Mochizuki, H. ; Morikawa, A.
Oxford, UK : Blackwell Publishing Ltd
Published 1997Staff ViewISSN: 1398-9995Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineType of Medium: Electronic ResourceURL: -
6Articles: DFG German National LicensesUeno, H ; Murphy, J ; Jass, J R ; Mochizuki, H ; Talbot, I C
Oxford UK : Blackwell Science Ltd.
Published 2002Staff ViewISSN: 1365-2559Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Tumour `budding' as an index to estimate the potential of aggressiveness in rectal cancer Aims: Although the characteristic of invasive pattern which contributes to Jass's classification is a sensitive prognostic marker in rectal cancer, reproducibility of its assessment has been shown to be problematic. As another histological parameter of invasive margin, we examined the prognostic significance of tumour ‘budding’ and attempted to establish its appropriate criteria. Methods and results: A total of 638 rectal cancer specimens was examined. We defined tumour `budding' as an isolated single cancer cell or a cluster composed of fewer than five cancer cells. We divided these into two groups by their intensity, i.e. the number of `budding' foci within a microscopic field of × 250. Rectal cancer with high-grade `budding' (≥ 10 foci in a field) was observed in 30.1% of patients, and was associated with lower 5-year survival rates (40.7%) than patients with low-grade `budding' (84.0%) (P 〈 0.0001). Based on multivariate analysis, tumour `budding' was selected as the significant independent variable, together with the number of nodes involved, extramural spread, lymphocytic infiltration, apical nodal involvement and tumour differentiation. Kappa coefficient of two-graded tumour `budding' in the intraobserver study was 0.84. Conclusions: Because of its value as a prognostic indicator and its reproducibility, tumour `budding' would be a good index to estimate the aggressiveness of rectal cancer.Type of Medium: Electronic ResourceURL: -
7Articles: DFG German National LicensesIto, T. ; Mochizuki, H. ; Kato, M. ; Nimura, Y. ; Hanai, T. ; Usuda, N. ; Hidaka, H.
Amsterdam : ElsevierStaff ViewISSN: 0003-9861Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyPhysicsType of Medium: Electronic ResourceURL: -
8Articles: DFG German National LicensesSugita, R. ; Mochizuki, H. ; Ito, T. ; Yokokura, H. ; Kobayashi, R. ; Hidaka, H.
Amsterdam : ElsevierStaff ViewISSN: 0006-291XSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyPhysicsType of Medium: Electronic ResourceURL: -
9Articles: DFG German National LicensesStaff View
ISSN: 0006-291XSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyPhysicsType of Medium: Electronic ResourceURL: -
10Articles: DFG German National LicensesStaff View
ISSN: 0014-5793Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyPhysicsType of Medium: Electronic ResourceURL: -
11Articles: DFG German National LicensesStaff View
ISSN: 0029-5493Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: Energy, Environment Protection, Nuclear Power EngineeringType of Medium: Electronic ResourceURL: -
12Articles: DFG German National LicensesStaff View
ISSN: 0029-5493Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: Energy, Environment Protection, Nuclear Power EngineeringType of Medium: Electronic ResourceURL: -
13Articles: DFG German National LicensesStaff View
ISSN: 0027-5107Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyMedicineType of Medium: Electronic ResourceURL: -
14Articles: DFG German National LicensesStaff View
ISSN: 0027-5107Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyMedicineType of Medium: Electronic ResourceURL: -
15Articles: DFG German National LicensesStaff View
ISSN: 0027-5107Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyMedicineType of Medium: Electronic ResourceURL: -
16Articles: DFG German National LicensesStaff View
ISSN: 0022-328XKeywords: Indenyl ; Metathesis ; Nitrosyl ; RutheniumSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: Chemistry and PharmacologyType of Medium: Electronic ResourceURL: -
17Articles: DFG German National LicensesStaff View
ISSN: 0022-328XKeywords: Indenyl ; Metathesis ; Nitrosyl ; RutheniumSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: Chemistry and PharmacologyType of Medium: Electronic ResourceURL: -
18Articles: DFG German National LicensesStaff View
ISSN: 0960-0760Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyType of Medium: Electronic ResourceURL: -
19Articles: DFG German National LicensesStaff View
ISSN: 0960-0760Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyType of Medium: Electronic ResourceURL: -
20Articles: DFG German National LicensesTakasaki, H. ; Miyamori, I. ; Nagai, K. ; Takeda, R. ; Mochizuki, H. ; Katagiri, M.
Amsterdam : ElsevierStaff ViewISSN: 0960-0760Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyType of Medium: Electronic ResourceURL: