Search Results - (Author, Cooperation:H. Lei)

2012-05-12

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Animals ; Cell Polarity/physiology ; Colorectal Neoplasms/genetics ; DNA-Binding Proteins/deficiency/genetics/metabolism ; Feedback, Physiological ; Female ; Frizzled Receptors/metabolism ; HEK293 Cells ; Humans ; Low Density Lipoprotein Receptor-Related Protein-6/metabolism ; Male ; Mice ; Mice, Knockout ; Oncogene Proteins/deficiency/genetics/metabolism ; Protein Stability ; Protein Structure, Tertiary ; Receptors, G-Protein-Coupled/deficiency/genetics/metabolism ; Receptors, Wnt/*metabolism ; Thrombospondins/*metabolism ; Ubiquitin-Protein Ligases/chemistry/*deficiency/genetics/*metabolism ; Ubiquitination ; Wnt Signaling Pathway ; Xenopus ; Zebrafish ; beta Catenin/metabolism

2018-05-15

The American Society for Microbiology (ASM)

0022-538X

1098-5514

Medicine

2018-10-17

American Physical Society (APS)

1098-0121

1095-3795

Physics

Superfluidity and superconductivity

2018-11-06

Institute of Physics (IOP)

1755-1307

1755-1315

Geography

Geosciences

Physics

2012-12-12

American Association for the Advancement of Science (AAAS)

0036-8075

1095-9203

Biology

Chemistry and Pharmacology

Computer Science

Medicine

Natural Sciences in General

Physics

Animals ; Arthropod Antennae/physiology ; Brain/physiology ; Electrophysiological Processes ; Feeding Behavior ; *Flowers ; Learning ; Male ; Manduca/*physiology ; Neurons/*physiology ; Octopamine/pharmacology/*physiology ; Odors ; Olfactory Pathways ; *Plant Nectar ; Pollination ; Smell/physiology ; Volatile Organic Compounds

1089-7623

AIP Digital Archive

Physics

Electrical Engineering, Measurement and Control Technology

A new electron cyclotron resonance (ECR) ion source (LECR3—Lanzhou Electron Cyclotron Resonance Ion Source No. 3) has been constructed this year. The main purpose of this source is to provide highly charged ion beams for atomic physics and surface physics research. The design of this ion source is based on the IMP 14.5 GHz ECR ion source (LECR2—Lanzhou Electron Cyclotron Resonance Ion Source No. 2) with double rf heating by inserting waveguide directly and aluminum chamber. Furthermore, the volume of the plasma chamber is larger than that of LECR2 so as to increase the rf power and improve beam intensity for highly charged ions. But the hexapole field on the chamber wall is kept the same value in order to compare with the performance of LECR2. After only four days conditioning the first test results were obtained. The final result of this ion source is expected to be better than LECR2's. © 2002 American Institute of Physics.

Electronic Resource

1365-3083

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Patients with systemic lupus erythematosus (SLE) were recently shown to be defective in costimulatory molecule CD80 (B7-1) expression on antigen-presenting cells. This study was undertaken to further investigate the expression and cytokine regulation of both CD80 and CD86 (B7-2) on monocytes from patients with SLE. Freshly isolated and in vitro cytokine-stimulated peripheral blood mononuclear cells from 13 patients with SLE and 10 healthy subjects were analysed, cytometrically with dual-fluorescence staining, to detect expression of CD80 and CD86 in the CD14+ monocyte population. The results showed that, as in normal individuals, an overwhelming majority (95.62 ± 3.54%) of monocytes from patients with SLE expressed the CD86 molecule, but only a few monocytes (5.54 ± 4.36%) had detectable CD80 expression. The effects of interleukin-10 (IL-10) on the expression of CD80 and CD86 on monocytes from patients with SLE and normal controls were similar. IL-10 down-regulated the expression of CD86 while it slightly enhanced that of CD80. Interferon-γ (IFN-γ) increased both CD80 and CD86 expression on monocytes from both SLE patients and normal groups, albeit less significantly in the former than in the latter, i.e. CD80: 142.84 ± 65.99% versus 226.08 ± 78.90%, P 〈 0.05; and CD86: 72.55 ± 74.23% versus 153.99 ± 94.14%, P 〈 0.05, when expressed as percentage modulation. Granulocyte–macrophage colony-stimulating factor (GM-CSF) showed a capacity for up-regulation of CD80 and CD86 expression on monocytes, of a magnitude that was similar both in patients with SLE and in normal subjects. We concluded that CD80 and CD86 were differentially expressed and modulated on monocytes and the defective IFN-γ-induced up-regulation of CD80 and CD86 expression on SLE monocytes might be a factor in the pathogenesis of SLE.

Electronic Resource

1365-2222

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Tachycardia and hypotension, two cardiovascular responses to anaphylaxis, were specifically induced by antigen in mice and rats, respectively, Intravenous injection of poly (Glu60Ala30Tyr10) (GAT) elicited tachycardia within 30–40 sec in GAT-primed B6 mice. Moreover, a minute amount of GAT (0.2μg) was enough to sensitize the mice to subsequent GAT-induced tachycardia. Challenging doses ranging from 100 ng to 500 μg could elicit tachycardia. The kinetics of tachycardia induction was different from that of antibody production or delayed-type hypersensitivity. Tachycardia was induced from day 6 after immunization, while delayed-type hypersensitivity developed as early as day 4, and anti-GAT antibodies were undetectable on day 6 and would not reach a maximum until day 8. Specific antigen-induced hypotension was also observed in rats. Furthermore, cardiovascular changes in both species could be passively transferred by heat-treated (56°C, 30 min) sera from immunized animals. These benchmarks of antigen-induced cardiovascular changes in mice or rats could be used as models to study the immune control of cardiovascular changes in anaphylactic responses.

Electronic Resource

1365-2222

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Background Increasing evidence suggests that pulmonary surfactant protein A (SP-A) and D (SP-D) participate in the lung defence against pathogens. However, the role of surfactant proteins in the pathogenesis of allergen-induced airway inflammation has not been elucidated. In this study we examined the levels and distributions of SP-A and SP-D in a dust mite (Dermatophagoides pteronyssinus, Der p) allergen-induced murine model of asthma.Methods The concentration of SP-A and SP-D in the bronchoalveolar lavage fluid (BALF) and the distribution of surfactant proteins in the lung were assayed by ELISA and immunohistochemistry methods, respectively. The effect of surfactant proteins on allergen-induced pulmonary lymphocyte proliferation was also studied.Results We demonstrated that there were marked reductions of SP-A and SP-D levels in the BALF of Der p-sensitized BALB/c mice at 48–72 h after allergen challenge (AC). Both purified SP-A and SP-D were able to suppress, in a dose dependent manner, Der p-stimulated intrapulmonary lymphocyte proliferation of naïve mice with saline or allergen challenge, or of Der p-sensitized mice with saline challenge. On the contrary, this suppressive effect was mild (〈 9%) on lymphocytes from sensitized mice after AC.Conclusion These results indicated the involvement of pulmonary surfactant proteins in the allergic bronchial inflammation of sensitized mice.

Electronic Resource

1365-2222

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Thrombocytopenia as well as hemoconcentration and leukopenia followed by leuko-cytosis were induced after HoGG challenge on HoGG-sensitized mice. Thrombocytopenia was induced within 2min and sustained for 1 day. HoGG-induced thrombocytopenia was not observed until day 10 after sensitization; mice challenged with HoGG dose ≥10μg developed thrombocytopenia. Two types of thrombocytopenia were observed in appropriately sensitized mice. HoGG induced thrombocytopenia at 2min and 60 min, whereas, α-macroglobulin induced thrombocytopenia at 2min, the platelet count of which returned to normal within 60min. Poly (Glu60Ala30 Tyr10) did not induce thrombocytopenia at 2min or 60min. The tracing study by 3H-serotonin labelled platelets demonstrated the 2min-sequestration of platelets in lungs or livers. The HoGG-induced sequestration of platelets at 2min was blocked by high dose heparin or Cobra Venom factor. Platelet activation at 60min was partially inhibited by dexamethasone, rhodostomin synthetic peptide 45–59, or platelet activation factor antagonist (WEB 2086). Furthermore, the thrombocytopenia could be transfered by heat (56°C, 4h)-treated immune sera. This suggests that HoGG-induced, non-IgE-mediated thrombocytopenia in anaphylaxis involves sequestration and activation of platelets. The sequestion in lungs occurs within 2min and can be inhibited by high dose heparin or Cobra Venom factor. The activation of platelets involves platelet activation factor, and fibrinogen receptor.

Electronic Resource

0001-4575

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Architecture, Civil Engineering, Surveying

Psychology

Electronic Resource

0022-0728

Oxygen reduction ; Platinum single-crystal electrodes

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Chemistry and Pharmacology

Electronic Resource

0022-0728

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Chemistry and Pharmacology

Electronic Resource

0022-0728

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Chemistry and Pharmacology

Electronic Resource

0022-0728

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Chemistry and Pharmacology

Electronic Resource

0022-3999

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Medicine

Psychology

Electronic Resource

0740-5472

amphetamine ; barbiturates ; caffeine ; cigarettes ; coffee ; drug dependence ; glue ; heroin ; nicotine ; polyaddiction ; tea ; tobacco

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Medicine

Psychology

Electronic Resource

1365-3083

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

This report describes the alteration of helper-suppressor balances in an immune response (Ir) gene-controlled system by varying the route and form of antigen injection. Adult responder BALB/c mice develop Lyt 1+2-, T cells for delayed-type hypersensitivity (DTH), and T-cell proliferative (Tprlf) responses to subcutaneous injection of either poly(Glu60Ala30Tyr10) (GAT)-coupled syngeneic spleen cells (GAT-SP) or GAT emulsified in complete Freund's adjuvant. In contrast, intravenous injection of adult responders with GAT-SP results in specific unresponsiveness for DTH, Tprlf, interleukin-2, and plaque-forming cell (PFC) responses. This tolerance is mediated by both suppressor T cells (Ts) and a functional clonal inhibition. Lyt 1-2+ Ts suppress the induction (afferent limb) of GAT-specific DTH and PFC but not Tprlf responses. The reduced T-cell proliferation observed in GAT-tolerant mice is due to a non-transferable mechanism(s), possibly functional clonal inhibition. Our data are compatible with a multi-step pathway involving both proliferating and non-proliferating helper T (Th) cells. In addition, the fine specificity of tolerance induction for DTH and Tprlf responses was examined by using the related antigens poly(Glu60Ala40) (GA) and poly(Glu50Tyr50) (GT). Tolerance is exquisitely specific, as GA tolerizes responses to GA and GAT, whereas GT tolerizes GAT but not GA responses. Thus, both the route and form of antigen administration are important to the induction and regulation of immune response in Ir gene-controlled systems. Possible mechanisms governing the Th/Ts balance and the induction of GAT-specific tolerance and suppression for cellular and humoral responses in adult responders are discussed.

Electronic Resource

1398-9995

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Background: There are detailed descriptions of the clinical experiences and prescriptions of asthma in traditional Chinese medicine. Xiao-qing-long tang (XQLT), or sho-seiryo-to by its Japanese name, is one of the Chinese herbal medicines used to treat bronchial asthma and allergic rhinitis for centuries. However, the therapeutic mechanisms of this medication are still far from clear. In this study, a house-dust-mite (Dermatophagoides pteronyssinus [Der p])-sensitized murine model of asthma was used to evaluate the immunomodulatory effect of XQLT on the allergen-induced airway inflammation in asthma. Methods: Three different protocols were designed to evaluate the treatment and/or long-term prophylactic effect of XQLT in Der p-sensitized mice. XQLT extracts (1 gm/kg) were administered to sensitized mice 1 h before allergen challenge (AC) (group A), 24 h after AC (group B), and every other day six times before AC (group C), respectively. Cellular infiltration and T-cell subsets in the bronchoalveolar lavage fluid (BALF) of allergen-challenged mice were analyzed. Intrapulmonary lymphocytes were also isolated to evaluate their response to allergen stimulation. Results: When XQLT was administered to the sensitized mice before AC (groups A and C), it suppressed airway inflammation by decreasing the number of total cells and eosinophil infiltration in the BALF, and downregulated the allergen- or mitogen-induced intrapulmonary lymphocyte response of sensitized mice as compared to those of controls. This immunomodulatory effect of XQLT may be exerted through the regulation of T-cell subsets by elevation or activation of the CD8+ and double-negative T-cell population in the lung. However, the administration of XQLT to sensitized mice 24 h after AC (group B) did not have the same inhibitory effect on the airway inflammation as XQLT given before AC. Conclusions: The administration of XQLT before AC has the immunomodulatory effect of reducing bronchial inflammation in the allergen-sensitized mice.

Electronic Resource

1570-7458

Trialeurodes vaporariorum ; Homoptera ; Aleyrodidae ; probing ; electrical penetration graph ; EPG ; honeydew excretion ; phloem ; feeding ; ingestion ; moulting

Springer Online Journal Archives 1860-2000

Biology

Abstract Probing behaviour of Trialeurodes vaporariorum (Westwood) larvae was monitored using the DC electrical penetration graph (EPG) technique on the host plant cucumber. EPGs were recorded for 16 h, simultaneously with honeydew excretion using a ‘honeydew clock’. Three waveforms were distinguished: a pathway waveform (C), and two phloem waveforms, one with a high (H), and one with a low frequency (L) signal. The C waveform mainly occurred in the crawler stage of the 1st instar larvae. EPGs recorded from larvae during and after moulting indicated that the process involves stylet withdrawal; hence the stylets of each new instar need to penetrate again from the leaf surface to the phloem. All sessile stages, from L1 to pre-pupa, spent almost their entire time in waveforms H and L. These waveforms alternated more frequently in the early instars than during the later ones, in which the H waveform became predominant. The H waveform was highly correlated with honeydew excretion and thus phloem sap ingestion. The L waveform was not related to honeydew excretion but EPGs indicated that the stylet tips remain in a sieve element during both waveforms. Periods of honeydew production demonstrated a delay of 30–40 min in relation to the onset and end of H and L waveforms. This delay is presumably related to the time needed for food passing through, or emptying of, the insect's gut. From the 1st instar to the pre-pupa, the frequency of excreted honeydew droplets decreased but their size increased, causing a net increase of the excretion rate.

Electronic Resource