Search Results - (Author, Cooperation:H. J. Mollenkopf)
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1Latest Papers from Table of Contents or Articles in PressP. Moura-Alves ; K. Fae ; E. Houthuys ; A. Dorhoi ; A. Kreuchwig ; J. Furkert ; N. Barison ; A. Diehl ; A. Munder ; P. Constant ; T. Skrahina ; U. Guhlich-Bornhof ; M. Klemm ; A. B. Koehler ; S. Bandermann ; C. Goosmann ; H. J. Mollenkopf ; R. Hurwitz ; V. Brinkmann ; S. Fillatreau ; M. Daffe ; B. Tummler ; M. Kolbe ; H. Oschkinat ; G. Krause ; S. H. Kaufmann
Nature Publishing Group (NPG)
Published 2014Staff ViewPublication Date: 2014-08-15Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Animals ; Anti-Bacterial Agents/metabolism ; Basic Helix-Loop-Helix Transcription Factors/*metabolism ; Bone Marrow Cells/cytology ; Cytokines/immunology/metabolism ; Feedback, Physiological ; Humans ; Ligands ; Macrophage Activation ; Mice ; Mycobacterium tuberculosis/growth & development/*immunology/metabolism ; Phenazines/metabolism ; Pigments, Biological/chemistry/*metabolism ; Pseudomonas Infections/metabolism ; Pseudomonas aeruginosa/*immunology/metabolism ; Pyocyanine/metabolism ; Receptors, Aryl Hydrocarbon/*metabolism ; Receptors, Pattern Recognition/*metabolism ; Virulence Factors/chemistry/metabolismPublished by: -
2Latest Papers from Table of Contents or Articles in PressJ. E. Galagan ; K. Minch ; M. Peterson ; A. Lyubetskaya ; E. Azizi ; L. Sweet ; A. Gomes ; T. Rustad ; G. Dolganov ; I. Glotova ; T. Abeel ; C. Mahwinney ; A. D. Kennedy ; R. Allard ; W. Brabant ; A. Krueger ; S. Jaini ; B. Honda ; W. H. Yu ; M. J. Hickey ; J. Zucker ; C. Garay ; B. Weiner ; P. Sisk ; C. Stolte ; J. K. Winkler ; Y. Van de Peer ; P. Iazzetti ; D. Camacho ; J. Dreyfuss ; Y. Liu ; A. Dorhoi ; H. J. Mollenkopf ; P. Drogaris ; J. Lamontagne ; Y. Zhou ; J. Piquenot ; S. T. Park ; S. Raman ; S. H. Kaufmann ; R. P. Mohney ; D. Chelsky ; D. B. Moody ; D. R. Sherman ; G. K. Schoolnik
Nature Publishing Group (NPG)
Published 2013Staff ViewPublication Date: 2013-07-05Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Adaptation, Physiological ; Anoxia/*genetics/metabolism ; Bacterial Proteins/genetics/metabolism ; Binding Sites ; Chromatin Immunoprecipitation ; Gene Expression Profiling ; *Gene Regulatory Networks/genetics ; Genomics ; Lipid Metabolism/genetics ; Metabolic Networks and Pathways/*genetics ; Models, Biological ; Mycobacterium tuberculosis/drug effects/*genetics/*metabolism/physiology ; Oxygen/pharmacology ; Proteolysis ; RNA, Messenger/genetics/metabolism ; Reproducibility of Results ; Sequence Analysis, DNA ; Transcription Factors/genetics/metabolism ; Tuberculosis/metabolism/microbiologyPublished by: -
3Latest Papers from Table of Contents or Articles in PressF. Meissner ; R. A. Scheltema ; H. J. Mollenkopf ; M. Mann
American Association for the Advancement of Science (AAAS)
Published 2013Staff ViewPublication Date: 2013-04-27Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyComputer ScienceMedicineNatural Sciences in GeneralPhysicsKeywords: Animals ; Lipopolysaccharides/immunology ; *Macrophage Activation ; Macrophages/*immunology ; Mass Spectrometry/*methods ; Mice ; Mice, Knockout ; Proteins/genetics/*secretion ; Proteome/genetics/*secretion ; Proteomics ; Toll-Like Receptor 4/*agonists ; Transcription, Genetic ; TranscriptomePublished by: -
4Articles: DFG German National LicensesJungblut, P. R. ; Schaible, U. E. ; Mollenkopf, H.-J. ; Zimny-Arndt, U. ; Raupach, B. ; Mattow, J. ; Halada, P. ; Lamer, S. ; Hagens, K. ; Kaufmann, S. H. E.
Oxford BSL : Blackwell Science Ltd
Published 1999Staff ViewISSN: 1365-2958Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: BiologyMedicineNotes: In 1993, the WHO declared tuberculosis a global emergency on the basis that there are 8 million new cases per year. The complete genome of the strain H37Rv of the causative microorganism, Mycobacterium tuberculosis, comprising 3924 genes has been sequenced. We compared the proteomes of two non-virulent vaccine strains of M. bovis BCG (Chicago and Copenhagen) with two virulent strains of M. tuberculosis (H37Rv and Erdman) to identify protein candidates of value for the development of vaccines, diagnostics and therapeutics. The mycobacterial strains were analysed by two-dimensional electrophoresis (2-DE) combining non-equilibrium pH gradient electrophoresis (NEPHGE) with SDS–PAGE. Distinct and characteristic proteins were identified by mass spectrometry and introduced into a dynamic 2-DE database (). Silver-stained 2-DE patterns of mycobacterial cell proteins or culture supernatants contained 1800 or 800 spots, respectively, from which 263 were identified. Of these, 54 belong to the culture supernatant. Sixteen and 25 proteins differing in intensity or position between M. tuberculosis H37Rv and Erdman, and H37Rv and M. bovis BCG Chicago, respectively, were identified and categorized into protein classes. It is to be hoped that the availability of the mycobacterial proteome will facilitate the design of novel measures for prevention and therapy of one of the great health threats, tuberculosis.Type of Medium: Electronic ResourceURL: -
5Articles: DFG German National LicensesStaff View
ISSN: 1432-0614Source: Springer Online Journal Archives 1860-2000Topics: BiologyProcess Engineering, Biotechnology, Nutrition TechnologyNotes: Abstract The outer membrane protein, PagC, of Salmonella typhimurium was converted into a secreted protein by linking the 61-amino-acid long, C-terminal signal sequence of the E. coli hemolysin protein (HlyAS) to the mature PagC peptide. This PagC-HlyAS fusion protein was expressed and efficiently secreted into the culture supernatant by E. coli upon complementation with the hemolysin secretion proteins HlyB and HlyD. Polyclonal antibodies raised against this fusion protein not only recognized PagC in the membrane fraction of all salmonellae by Western blotting, but also reacted with proteins of smaller size in other gram-negative bacteria tested. A monoclonal antibody against the PagC-HlyAS fusion protein recognized only PagC in membrane fractions. The antibody-binding domain was determined using synthetic peptides derived from specific PagC domains. Sera from Salmonella-infected human patients and from a rabbit infected with S. typhimurium did not react with PagC in immunoblots, suggesting that PagC may not be recognized as a major antigen by the humoral immune system.Type of Medium: Electronic ResourceURL: