Search Results - (Author, Cooperation:Goy)

article

1986

Schuljahr 02 ; Grundschule ; Primarbereich ; Unterrichtsentwurf ; Unterrichtsmaterial ; Text ; Bild ; Kunstunterricht ; Visuelle Kommunikation ; Ästhetische Bildung ; Sachunterricht ; Gestaltung ; Tür

BDK-Mitteilungen, Bd. 22 (1986) H. 1, S. 25-27, 0005-2981

German

1476-4431

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Electronic Resource

1471-4159

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Abstract: Bioassay analysis of extracts of the major neurose-cretory structures of the American lobster have revealed several different agents with stimulatory effects on the cyclic GMP metabolism of various lobster tissues. The most potent of these is a peptide extracted from the sinus gland, a neurohemal organ found in the animal's eyestalk. This molecule, called peptide G1 (for its effects on cyclic GMP metabolism), can increase the cyclic GMP content of every lobster tissue tested, sometimes by as much as 200-fold. In this article, we describe the purification and some of the chemical properties of peptide G1. Purification was accomplished by sequential anion exchange and reverse-phase HPLC. The purified peptide is a large, extremely hydrophobic molecule. Its apparent molecular mass on a reducing sodium dodecyl sulfate-containing gel is 6.4 kDa, and its calculated molecular mass (based on an amino acid analysis of the purified material) is 8.2 kDa. Amino acid analysis reveals a high proportion of leucine and valine residues. The amino terminus of the molecule is not susceptible to Edman degradation, but sequencing studies were successfully carried out on tryptic fragments. Based on the estimated size of the molecule, these studies provide ∼60% of the total sequence. No homologies with any previously sequenced peptide were observed, but biochemical similarities to as yet unsequenced peptides found in extracts of sinus glands from other crustaceans (hyperglycemic hormone and moult-inhibiting hormone) are described.

Electronic Resource

1471-4159

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Abstract: Several observations suggest that cyclic GMP might regulate some aspect of neuromuscular physiology or metabolism in the lobster, Homarus americanus: (1) lobster muscle is one of the richest known sources of cyclic GMP-dependent protein kinase, (2) the preparation contains several phosphoproteins whose state of phosphorylation is affected by cyclic GMP more effectively than by cyclic AMP, and (3) guanylate cyclase and phosphodiesterase are active in this tissue. However, no factor has yet been identified that alters lobster muscle cyclic GMP levels. We have screened extracts of neural and neurosecretory structures for the capacity to promote cyclic GMP accumulation in isolated exoskeletal muscles. Extracts of the sinus gland (a neurohemal organ found in the eyestalk) contain a factor that induces up to 100-fold increases in muscle cyclic GMP content, whereas extracts of other tissues are ineffective. This factor can also act on targets other than muscle, with hepatopancreas, testis, and neuronal tissue showing the largest responses. The sinus gland factor does not appear to affect cyclic GMP metabolism by depolarizing the preparation or by mobilizing extracellular Ca2+. The effect on cyclic GMP levels is dose-dependent and linear with time. Biological activity is destroyed by boiling and by 90% ethanol. It is also destroyed by trypsin, chymotrypsin, or pronase, which suggests that the factor is a protein or peptide. Both gel filtration chromatography and experiments using dialysis tubing with different molecular weight exclusion limits indicate that the factor has an apparent molecular weight of 5,000–12,000 daltons. A preliminary fractionation scheme, based on gel filtration, ion-exchange, and reverse-phase chromatography, gives 〉1,300-fold purification. Our long-range goal is to purify this factor to homogeneity, compare it to other peptide hormones, and use it as a probe to evaluate the role of cyclic GMP at the neuromuscular junction.

Electronic Resource

1540-8183

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

We describe our initial experience with the “Big Skinny” angioplasty catheter, currently only available as an investigational device and in sizes of 4.5, 5, and 6 mm. Six patients with severe angina pectoris were successfully dilated with this angioplasty catheter and have remained asymptomatic up until a mean follow-up period of 6 months. However, because of the large profile of the catheter, sufficient back-up with an adequate guiding catheter should be obtained and predilation with a smaller angioplasty catheter should be performed. The use of this catheter may be a more simple alternative for the treatment of oversized vessel stenosis than the “hugging balloon” technique or directional atherectomy. Otherwise, it may be considered as an adjunct technique in order to optimize the angiographic result after stenting or directional atherectomy in this particular indication. (J Interven Cardiol 1995; 8:233–237)

Electronic Resource

1540-8183

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

This is a review on prospective randomized comparisons of PTCA and stents in the treatment of de novo native coronary artery lesions. BENESTENT and STRESS, two multicentric studies, used the articulated Palmaz-Schatz™ stent. In Lausanne, a single center trial limited to right coronary artery lesions, was conducted using the Wiktor™ stent. During the in-hospital phase, BENESTENT and STRESS showed the composite clinical end point to be less in the stent than in the PTCA groups (P 〈 0.05). In Lausanne, there was no difference between groups. The incidence of subacute closure was similar with both treatments in three trials. Angiographically, both postpro-cedural minimal luminal diameter (MLD) and percentage stenosis were larger in the stent group (P 〈 0.05). At 6 months, in both BENESTENT and STRESS, a composite clinical end point was reached by less stent patients than PTCA patients, with a reduced need for repeat nonsurgical reintervention by Stenting. However, in Lausanne, there was no difference between stent and PTCA groups. At 6 months in both BENESTENT and STRESS, a persistent lower MLD, a larger percentage of stenosis, and a higher incidence of angiographic restenosis were found in the PTCA groups (P 〈 0.05). In Lausanne, no differences in MLD, percentage stenosis, and angiographic restenosis were found between groups. Effective Stenting of de novo lesions does improve immediate results compared to conventional balloon PTCA. The long-term outcome of Stenting with Palmaz-Schatz™ stents is also improved compared to PTCA.

Electronic Resource

1540-8183

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Electronic Resource

1540-8167

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

2:1 AV Block in LQTS. Introduction Conduction abnormalities associated with long QT syndrome (LQTS) have been reported as “pseudo 2:1 AV block” due to sinus intervals shorter than ventricular refractoriness. Method and Results: We report the electrophysiologic characteristics of a patient suffering from congenital LQTS with episodes of true 2:1 AV block. Induction of 2:1 infra-Hisian blocks and return to 1:1 conduction were observed using single atrial and ventricular extrastimuli. The block was located in the Purkinje network but not in the myocardium. Conclusion: The His-Purkinje system of our LQTS patient displayed dynamic properties with a strong increase in refractoriness for short-long sequences and a decrease for long-short sequences that triggered intermittent 2:1 AV blocks.

Electronic Resource

1540-8183

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

The goal of this follow-up study was to assess the long-term survival of all patients having undergone a first PTCA between 1981 and 1990 and to relate the outcome to the baseline clinical and angiographic state. Although PTCA has become a widely accepted therapeutic choice for revascularization, the authors lacked information on long-term outcome. Data was collected by questionnaire, the end points being a second PTCA, MI, CABG, death or any of these events. The survival curves were constructed using the Kaplan-Meier method. Multivariate analysis was performed by a Cox proportional hazards model. Complete follow-up data were collected for 1,071 patients for a mean period of 7.4 years (SEM ± 1.98 months) with a range of 0 to 14 years. Mean age was 57 years. PTCA was successful in 85% of patients. In-hospital event rates were death 1.3%, MI 4.4 %, and emergency CABG 2.9%. Overall survival at 14 years was 69% (SEM ± 9.6%) and event-free survival was 47% (SEM ± 5.8%). MI rate was 11%, CABG 15%, and 20% of patients underwent repeat PTCA. Presence of cardiovascular risk factors, poor left ventricular ejection fraction, and prior CABG were significantly associated with poorer event-free survival. The short-term observations are consistent with results reported by the other follow-up studies. In addition, the study found a total survival rate 14 years after a first PTCA of 69% and 47% of the cohort remained event free.

Electronic Resource

1540-8183

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

The safety and efficacy of percutaneous transluminal coronary angioplasty (PTCA) was examined in a group of 91 octogenarians during 133 consecutive procedures and compared to the younger part of our PTCA population (3,443 procedures) during an 8-year study period. Most octogenarians presented with refractory or unstable angina pectoris and multivessel involvement, but had normal left ventricular function. The angiographic success rate was 84%. Technical failure was observed in 12% of cases and complications in 14%. Two early procedure related deaths were observed. Restenosis was estimated at 24%. The proportion surviving and the proportion without cardiac event estimated by the Kaplan-Meier method at 2 years were 0.89 and 0.60, respectively. In comparison to the younger population, the immediate angiographic success was lower as a result of a higher incidence of technical failure (P 〈 0.05). Otherwise, no difference in terms of restenosis or overall related complication rate was noted (P 〉 0.1). It is concluded that PTCA is an efficient and safe therapeutic option for octogenarians with severe or refractory angina pectoris. Technical failure is more frequently observed than in younger patients and explains a lower immediate angiographic success. Nevertheless, at 2-years follow-up, a large majority of patients are alive and asymptomatic.

Electronic Resource

1471-4159

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Abstract: Cytoplasmic, nitric oxide-activated guanylate cyclases are expressed in many regions of the mammalian brain and are thought to participate in functions as diverse as synaptogenesis and long-term potentiation. In this study, we have characterized cytoplasmic guanylate cyclases in the nervous system of an invertebrate, the American lobster. Cytoplasmic cyclase specific activity is higher in lobster nerve cord than in any other lobster tissue tested, and considerably higher than in typical rat tissues (cerebellum, lung, and liver). However, nitric oxide donors have minimal effects on lobster nerve cord cyclic GMP production, when applied either to intact tissue or to cytoplasmic extracts. Parallel immunocytochemical studies, using an anti-cyclic GMP antibody, reveal that only a small subset of lobster neurons responds to nitric oxide with a significant elevation of cyclic GMP levels. HPLC analysis of nerve cord cytoplasm reveals two chromatographically separable cyclases, a minor nitric oxide-sensitive form whose retention time is identical to that of the conventional mammalian enzyme and a more abundant nitric oxide-insensitive form that appears to be novel. The physiological function and phylogenetic distribution of this nitric oxide-insensitive enzyme, and the signaling mechanisms that regulate its activity, are not known.

Electronic Resource

1460-9592

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Summary Background: EMLA cream is the current technique of choice to reduce pain during venous cannulation in most paediatric practice. Its use is limited by logistic arrangements and failure to improve cooperation and allay anxiety. Nitrous oxide (N2O) would appear to be an effective alternative. A combination technique may be useful in selected patients. Methods: One hundred and twenty unpremedicated ASA 1 and 2 day surgery patients, aged 8–15 years were randomized into group 1 (EMLA + air/O2), group 2 (50% N2O/50% O2) and group 3 (EMLA + 50% N2O/50% O2). All patients underwent cannulation on the dorsum of the hand with a 22-G intravenous catheter. Pain behaviour before cannulation was assessed by an observer with Children's Hospital of Eastern Ontario Pain Scale (CHEOPS). Pain during cannulation was evaluated with CHEOPS by an observer and Visual Analogue Scale (VAS) (0–100 mm) by the patient. Satisfaction score (0–100%) for the experience were reported by the patient. Degree of ease of cannulation, time for cannulation were assessed. Heart rate, oxygen saturation were compared before, during and after cannulation. Results: The self-reported VAS for group 3 (10.10 ± 14.99) was significantly lower than group 1 (26.13 ± 27.59) and group 2 (18.35 ± 18.11) (P = 0.003). No significant difference existed between VAS for group 1 and 2. There were also significantly more patients with VAS = 0 in group 3 (23/40) versus group 2 (11/40) versus group 1 (10/40), P = 0.004. The satisfactory score in group 3 (93 ± 9.96) was significantly higher (P = 0.039) than group 1 (81.13 ± 24.61) and group 2 (84 ± 22.02). The increase in CHEOPS from before to during cannulation was significant only in group 1 (P = 0.002). There was no significant difference between frequency of patients with side-effects, ease of cannulation and time taken for cannulation in the three groups. Conclusions: EMLA and 50% N2O are equally effective for pain reduction while a combination technique provides superior analgesia and satisfaction. N2O has an advantage over EMLA in reduction of pain related behaviour in older children.

Electronic Resource

1749-6632

Blackwell Publishing Journal Backfiles 1879-2005

Natural Sciences in General

Electronic Resource

0026-7937

Linguistics and Literary Studies

REVIEWS

1546-1696

Nature Archives 1869 - 2009

Biology

Process Engineering, Biotechnology, Nutrition Technology

[Auszug] Analyzing European field trials reveals that companies and regulators alike have been responsive to environmental ...

Electronic Resource

1540-8167

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

The development of dual-chamber rate responsive pacing is the logical consequence of technical and clinical developments and research in pacemaker technology. The first rate responsive dual-chamber pacemaker was implanted in June 1986 and the successful performance of this device encouraged manufacturers to further develop this technology. The rhythmic corrections that could be achieved were a strong argument to make use of this new technology in patients suffering from combined sinus node and AV nodal disease. DDD rate responsive pacemakers, therefore, have been implanted in 16 patients with a mean follow-up of 10.4 months. No technical complications were encountered, 2 patients died from causes not related to an arrhythmic problem. Of the 14 remaining patients, 12 are still in a dual-chamber rate responsive mode, 2 are in DDD or DDI-mode because of chronotropic competence of the sinus node. Therefore, we, conclude that dual-chamber rate responsive pacing is a reliable mode for long-term physiological pacing. Different features that can be included in a DDDR pacemaker may widen its use so that 85% of pacemaker indications might be covered with the DDDR pacemaker.

Electronic Resource

1476-4687

Nature Archives 1869 - 2009

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

[Auszug] In both prokaryotes and eukaryotes methyl groups can be added to and removed from the carboxyl groups of proteins. Recent work has revealed that these reactions have a role in several behavioural phenomena. The nature of this role has been uncovered in one case—that of bacterial ...

Electronic Resource

0375-9601

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Physics

Electronic Resource

0009-8981

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Medicine

Electronic Resource

0009-8981

Detoxification enzymes ; Lipid peroxide ; Malondialdehyde ; Myocardial infarction

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Medicine

Electronic Resource