Search Results - (Author, Cooperation:G. Palfi)
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1Latest Papers from Table of Contents or Articles in PressM. E. Allentoft ; M. Sikora ; K. G. Sjogren ; S. Rasmussen ; M. Rasmussen ; J. Stenderup ; P. B. Damgaard ; H. Schroeder ; T. Ahlstrom ; L. Vinner ; A. S. Malaspinas ; A. Margaryan ; T. Higham ; D. Chivall ; N. Lynnerup ; L. Harvig ; J. Baron ; P. Della Casa ; P. Dabrowski ; P. R. Duffy ; A. V. Ebel ; A. Epimakhov ; K. Frei ; M. Furmanek ; T. Gralak ; A. Gromov ; S. Gronkiewicz ; G. Grupe ; T. Hajdu ; R. Jarysz ; V. Khartanovich ; A. Khokhlov ; V. Kiss ; J. Kolar ; A. Kriiska ; I. Lasak ; C. Longhi ; G. McGlynn ; A. Merkevicius ; I. Merkyte ; M. Metspalu ; R. Mkrtchyan ; V. Moiseyev ; L. Paja ; G. Palfi ; D. Pokutta ; L. Pospieszny ; T. D. Price ; L. Saag ; M. Sablin ; N. Shishlina ; V. Smrcka ; V. I. Soenov ; V. Szeverenyi ; G. Toth ; S. V. Trifanova ; L. Varul ; M. Vicze ; L. Yepiskoposyan ; V. Zhitenev ; L. Orlando ; T. Sicheritz-Ponten ; S. Brunak ; R. Nielsen ; K. Kristiansen ; E. Willerslev
Nature Publishing Group (NPG)
Published 2015Staff ViewPublication Date: 2015-06-13Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Archaeology/methods ; Asia/ethnology ; Asian Continental Ancestry Group/*genetics ; Cultural Evolution/*history ; DNA/genetics/isolation & purification ; Europe/ethnology ; European Continental Ancestry Group/*genetics ; *Fossils ; Gene Frequency/genetics ; Genetics, Population ; Genome, Human/*genetics ; *Genomics ; History, Ancient ; Human Migration/history ; Humans ; Lactose Intolerance/genetics ; Language/*history ; Polymorphism, Single Nucleotide/genetics ; Skin Pigmentation/geneticsPublished by: -
2Articles: DFG German National LicensesStaff View
ISSN: 0304-4211Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyType of Medium: Electronic ResourceURL: -
3Articles: DFG German National LicensesStaff View
ISSN: 1432-1904Source: Springer Online Journal Archives 1860-2000Topics: BiologyChemistry and PharmacologyNatural Sciences in GeneralType of Medium: Electronic ResourceURL: -
4Articles: DFG German National LicensesStaff View
ISSN: 1432-1904Source: Springer Online Journal Archives 1860-2000Topics: BiologyChemistry and PharmacologyNatural Sciences in GeneralType of Medium: Electronic ResourceURL: -
5Articles: DFG German National LicensesStaff View
ISSN: 1476-4687Source: Nature Archives 1869 - 2009Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsNotes: [Auszug] Wheat variety 'Besostaia 1', maize variety 'Szegedi sarga lofogu' and rice variety 'Dubovskij 129' were included in the experiments. Bleeding sap was collected at the flowering stage during the night. The sterile sap was subjected to one- or two-dimensional paper chromato-graphy (ascending ...Type of Medium: Electronic ResourceURL: -
6Articles: DFG German National LicensesStaff View
ISSN: 1573-0778Keywords: cell culture ; cellulose sulphate ; encapsulation ; monoclonal antibodies ; poly-dimethyl-diallyl-ammoniumchlorideSource: Springer Online Journal Archives 1860-2000Topics: BiologyMedicineProcess Engineering, Biotechnology, Nutrition TechnologyNotes: Abstract A new encapsulation method was developed for the cultivation of mammalian cells. The capsules were produced using a solution of sodium cellulose sulphate (CS)(1.5%) and poly-dimethyl-diallyl-ammoniumchloride (PDMDAAC). When CS droplets fell into the precipitation bath consisting of a 2% solution of PDMDAAC, immediately a membrane at the interphase was built up. The influences of varying encapsulation process parameters on capsule characteristics, cell growth, and monoclonal antibody production were tested. This new method showed advantages when compared to other methods mainly due to time simplicity of the whole process.Type of Medium: Electronic ResourceURL: -
7Articles: DFG German National LicensesStaff View
ISSN: 1573-5036Source: Springer Online Journal Archives 1860-2000Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionType of Medium: Electronic ResourceURL: -
8Articles: DFG German National LicensesStaff View
ISSN: 1573-5036Source: Springer Online Journal Archives 1860-2000Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: Summary During the study of the free amino acid spectrum of diseased rice, soybean, potato and tobacco leaves, an unknown compound was detected, the occurrence of which was correlated with an abnormal physiological condition of the plants or with some disturbance in amino acid or protein metabolism. On chromatograms this substance stains blue following fixation with cupric nitrate. After purification with the aid of bacteria, this ‘blue substance’ was identified as pipecolic acid. Since pipecolic acid could be found in the leaves of rice sprayed with maleic hydrazide, but not in control plants, it would seem that it is a metabolic product of growth inhibition.Type of Medium: Electronic ResourceURL: -
9Articles: DFG German National LicensesStaff View
ISSN: 1573-5036Source: Springer Online Journal Archives 1860-2000Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: Summary 1. On the basis of new theoretical considerations developed by us a new and reliable method has been elaborated for the rapid determination of water deficit in plant cultures. 2. In paper-chromatograms of plants optimally supplied with water free proline is shown to be present in very small amounts, usually only as traces. Comparative experiments showed that in cases of water shortage the proline content of the leaves of the plant strikingly increases. This commonly occurs in every phase of development of mono- and dicotyledons. 3. Drought-resistant plants synthetize more proline than varieties less resistant to water deficit when equally water deficient. 4. By a method which does not require chromatography the procedure of tests has been accelerated so that leaf extract containing amino acid mixture was dropped onto a chromatogram paper and immediately treated with isatine. In this test the effect of slight, permanent or medium water deficit upon proline content is so great that extracts react with isatine to give an intense dark blue colour which suppresses the reddish-brown colour of other amino acids. 5. To avoid the necessity of preparing isatine reagent frequently, because it loses its amino acid sensitivity very rapidly, a new isatine solution has been formulated. Our isatine-paper indicator prepared with this solution keeps its reactivity with amino acids 5 to 6 months. 6. An unusually high proline concentration occurs also if the salt content of irrigation water or also of the soil is high. The increase of the proline level indicates therefore physiological dryness.Type of Medium: Electronic ResourceURL: -
10Articles: DFG German National LicensesStaff View
ISSN: 1573-5036Source: Springer Online Journal Archives 1860-2000Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: Summary 1. The amino acid concentration of the upper leaves of rice plants without fertilization and supplied with 434 kg ammonium sulphate, per hectare is higher than that of the lower ones. In rice manured with 695 kg per hectare the amino acid concentration in all leaves is almost the same. Great amounts of N tend to level off the differences in free amino acids in the leaves at various insertions. 2. The asparagine concentration of the leaves increases parallel with the level of N-nutrition. 3. A new ninhydrine and isatin positive amino-acid-like compound was detected atR f 0,64 which is not a protein constituent. The amount of this substance, denoted as β, increased with the increased level of N-nutrition. It was not found in the untreated control. High N-doses result in the appearance of symptoms of P deficiency. The level of N-supply is hardly reflected in the N-concentration of the leaves. On the contrary, it is indicated well by the dry weight and total-N content.Type of Medium: Electronic ResourceURL: -
11Articles: DFG German National LicensesMerten, O.-W. ; Moeurs, D. ; Keller, H. ; Leno, M. ; Palfi, G. E. ; Cabanié, L . ; Couvé, E.
New York, NY [u.a.] : Wiley-Blackwell
Published 1994Staff ViewISSN: 0006-3592Keywords: hybridoma ; subclone ; continuous culture ; batch culture ; igG-mRNA ; biosynthetic activities ; antibody production ; Chemistry ; Biochemistry and BiotechnologySource: Wiley InterScience Backfile Collection 1832-2000Topics: BiologyProcess Engineering, Biotechnology, Nutrition TechnologyNotes: During long-term continuous culture of the hybridoma cell line 11317, a better-producing subclone (I1317-SF11), giving improved productivity, has been selected. The comparison of the original cell line (I1317-DC) with this subclone revealed that although the growth patterns of both clones were similar, both in continuous and in batch cultures, considerable differences could be seen between the clones with respect to monoclonal antibody (MAB) accumulation, MAB production rate, the levels of mRNA coding for heavy and light chains of IgG, and some metabolic activities. In continuous culture as well as in batch culture, I1317-SF11 showed increased levels of mRNA coding for kappa and gamma chains compared with I1317-DC and/or a modified ratio of the mRNA species when compared to that in I1317-DC. Using pulse experiments, it could be established that the biosynthesis of both chains was augmented in I1317-SF11. Although the kappa and gamma mRNA levels were modified or inversed for I1317-SF11, the cells always synthesized more kappa than gamma chains. The overall increase in the synthetic activity of I1317-SF11 is suggested as one reason for the considerable increase of IgG productivity and product accumulation in continuous culture as well as in repeated batch cultures. Tests concerning metabolic activity revealed that I1317-SF11 had a predominantly glycolytic metabolism independent of growth requirements, whereas for I1317-DC the metabolism became increasingly glycolytic with increased growth. The antibody yield coefficient of I1317-SF11 on glutamine was significantly higher than that of I1317-DC for the continuous culture, whereas the antibody coefficients on glucose were almost similar for both clones under the different culture conditions used. Both antibody coefficients were considerablly influenced by the specific growth rate.All these facts together lead to the conclusion that subclone I1317-SF11 uses more of the energy available, or it was the energy and/or precursors available for the synthesis and production of MAB more efficiently than the thesis and production of MAB more efficiently than the original cell line. Although the levels of mRNA coding for heavy and light chains of IgG were modified, it could be confirmed that the overall regulation of MAB-synthesis and -production occurs post-translationally and that at higher growth rates, more biosynthetic activity is diverted to biomass production. © 1994 John Wiley & Sons, Inc.Additional Material: 9 Ill.Type of Medium: Electronic ResourceURL: