Search Results - (Author, Cooperation:G. Mignot)

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  1. 1
    Staff View
    Publication Date:
    2011-12-17
    Publisher:
    American Association for the Advancement of Science (AAAS)
    Print ISSN:
    0036-8075
    Electronic ISSN:
    1095-9203
    Topics:
    Biology
    Chemistry and Pharmacology
    Computer Science
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Adenosine Triphosphate/metabolism ; Animals ; Antineoplastic Agents/*pharmacology/therapeutic use ; Autophagy/drug effects/*physiology ; Calreticulin/pharmacology ; Cell Death/immunology ; Cell Line, Tumor ; Dendritic Cells/immunology ; Humans ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Mitoxantrone/pharmacology ; Neoplasms/drug therapy/*immunology
    Published by:
    http://www.aaas.org/

    Latest Papers from Table of Contents or Articles in Press
  2. 2
    Staff View
    Publication Date:
    2013-11-23
    Publisher:
    American Association for the Advancement of Science (AAAS)
    Print ISSN:
    0036-8075
    Electronic ISSN:
    1095-9203
    Topics:
    Biology
    Chemistry and Pharmacology
    Computer Science
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Adoptive Transfer ; Animals ; Anti-Bacterial Agents/administration & dosage ; Antineoplastic Agents/*therapeutic use ; Bacterial Translocation/*drug effects ; Cyclophosphamide/*therapeutic use ; Germ-Free Life ; Gram-Positive Bacteria/drug effects/physiology ; Immunologic Memory ; Immunosuppressive Agents/*therapeutic use ; Intestine, Small/*microbiology ; Lymphoid Tissue/immunology/microbiology ; Mice ; Microbiota/drug effects/*physiology ; Neoplasms/*drug therapy/*immunology ; Th17 Cells/immunology/transplantation
    Published by:
    http://www.aaas.org/

    Latest Papers from Table of Contents or Articles in Press
  3. 3
    Ganne, V. ; Mignot, G.
    Springer
    Published 1991
    Staff View
    ISSN:
    1573-0778
    Keywords:
    CHO cells ; DOEHLERT matrix ; experimental design ; Factor VIII ; HADAMARD matrix ; mathematical model ; optimization ; serum-free medium
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Medicine
    Process Engineering, Biotechnology, Nutrition Technology
    Notes:
    Abstract Selection and optimization of the concentrations of chemically defined components effective on recombinant factor VIII:C production by CHO cells have been performed with statistically designed experiments. Screening of efficient compounds on factor VIII expression was performed by using HADAMARD method, while a precise optimization of the concentrations of two components was achieved with the help of DOEHLERT method. Increased specific activity of factor VIII and reduced cost medium have been obtained with a fewer number of experiments than compared to a less rational approach.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/BF00624762

    Articles: DFG German National Licenses
  4. 4
    Staff View
    ISSN:
    1573-739X
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Chemistry and Pharmacology
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/BF01876919

    Articles: DFG German National Licenses
  5. 5
    Staff View
    ISSN:
    1573-0778
    Keywords:
    continuous culture ; free solution capillary electrophoresis ; hollow fibers ; monoclonal antibody characterization ; protein-free medium
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Medicine
    Process Engineering, Biotechnology, Nutrition Technology
    Notes:
    Abstract The performance of a protein-free medium was compared in culture flasks with a serum-supplemented medium and with a serum free medium in terms of cell growth and monoclonal antibody production by a murine hybridoma. We present results of continuous production in hollow fiber culture systems using serum-free medium and protein-free medium. In protein-free medium, it has been possible to produce large quantities of monoclonal antibody with a productivity similar to that obtained in serum-free medium. After a two steps purification process, monoclonal antibodies were characterized by SDS-PAGE, High Performance Size Exclusion Chromatography and Free Solution Capillary Electrophoresis. SDS-PAGE and high performance chromatography analysis have showed that purified monoclonal antibodies produced in serum-free medium or protein-free medium were similar. Furthermore, Capillary Electrophoresis characterization revealed that both MAbs were constituted by three isoforms with equivalent electrophoretic mobilities.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/BF00376098

    Articles: DFG German National Licenses
  6. 6
    Staff View
    ISSN:
    1573-0778
    Keywords:
    CHO cells ; macroporous microcarrier ; scale-up ; serum-free ; Von Willebrand factor
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Medicine
    Process Engineering, Biotechnology, Nutrition Technology
    Notes:
    Abstract Recombinant Chinese hamster ovary cells producing Von Willebrand factor have been successfully grown in gelatin macroporous microcarriers (Cultispher-G). Serum-free cultures were maintained in 1, 4, and 10 liter fermentors for more than two months. Comparative studies with Cytodex-3 microcarriers have been performed in 1 liter fermentors. The lower specific Von Willebrand factor productivity of CHO cells cultivated on Cultispher-G were offset by higher cell densities (107−2×107 cells/ml). Volumetric Von Willebrand factor productivity was influenced by oxygen concentration, and remained stable during scale-up from 1 to 10 liter fermentors.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/BF00365097

    Articles: DFG German National Licenses