Search Results - (Author, Cooperation:G. J. Bryan)

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  1. 1
    X. Xu ; S. Pan ; S. Cheng ; B. Zhang ; D. Mu ; P. Ni ; G. Zhang ; S. Yang ; R. Li ; J. Wang ; G. Orjeda ; F. Guzman ; M. Torres ; R. Lozano ; O. Ponce ; D. Martinez ; G. De la Cruz ; S. K. Chakrabarti ; V. U. Patil ; K. G. Skryabin ; B. B. Kuznetsov ; N. V. Ravin ; T. V. Kolganova ; A. V. Beletsky ; A. V. Mardanov ; A. Di Genova ; D. M. Bolser ; D. M. Martin ; G. Li ; Y. Yang ; H. Kuang ; Q. Hu ; X. Xiong ; G. J. Bishop ; B. Sagredo ; N. Mejia ; W. Zagorski ; R. Gromadka ; J. Gawor ; P. Szczesny ; S. Huang ; Z. Zhang ; C. Liang ; J. He ; Y. Li ; Y. He ; J. Xu ; Y. Zhang ; B. Xie ; Y. Du ; D. Qu ; M. Bonierbale ; M. Ghislain ; R. Herrera Mdel ; G. Giuliano ; M. Pietrella ; G. Perrotta ; P. Facella ; K. O'Brien ; S. E. Feingold ; L. E. Barreiro ; G. A. Massa ; L. Diambra ; B. R. Whitty ; B. Vaillancourt ; H. Lin ; A. N. Massa ; M. Geoffroy ; S. Lundback ; D. DellaPenna ; C. R. Buell ; S. K. Sharma ; D. F. Marshall ; R. Waugh ; G. J. Bryan ; M. Destefanis ; I. Nagy ; D. Milbourne ; S. J. Thomson ; M. Fiers ; J. M. Jacobs ; K. L. Nielsen ; M. Sonderkaer ; M. Iovene ; G. A. Torres ; J. Jiang ; R. E. Veilleux ; C. W. Bachem ; J. de Boer ; T. Borm ; B. Kloosterman ; H. van Eck ; E. Datema ; B. Hekkert ; A. Goverse ; R. C. van Ham ; R. G. Visser
    Nature Publishing Group (NPG)
    Published 2011
    Staff View
    Publication Date:
    2011-07-12
    Publisher:
    Nature Publishing Group (NPG)
    Print ISSN:
    0028-0836
    Electronic ISSN:
    1476-4687
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Evolution, Molecular ; Gene Duplication ; Gene Expression Regulation, Plant ; Genes, Plant/genetics ; Genetic Variation ; Genome, Plant/*genetics ; *Genomics ; Haplotypes/genetics ; Heterozygote ; Homozygote ; Immunity, Innate ; Inbreeding ; Molecular Sequence Annotation ; Molecular Sequence Data ; Plant Diseases/genetics ; Ploidies ; Solanum tuberosum/*genetics/physiology
    Published by:
    http://www.nature.com/

    Latest Papers from Table of Contents or Articles in Press
  2. 2
    Staff View
    Publication Date:
    2018-10-04
    Publisher:
    Genetics Society of America (GSA)
    Electronic ISSN:
    2160-1836
    Topics:
    Biology
    Published by:
    http://www.genetics-gsa.org/

    Latest Papers from Table of Contents or Articles in Press
  3. 3
    Staff View
    ISSN:
    1432-2242
    Keywords:
    Key words Wheat ; PCR ; Microsatellite ; Simple sequence repeats
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Notes:
    Abstract  The development of large panels of simple-to-analyse genetic markers for tagging agronomically important genes and diversity studies in hexaploid bread wheat is an important goal in applied cereal genetic research. We have isolated and sequenced over 200 clones containing microsatellites from the wheat genome and have tested 153 primer pairs for genetic polymorphism using a panel of ten wheat varieties, including the parents of our main mapping cross. A subset comprising 49 primer pairs detects 76 loci, of which 74 can be unequivocably allocated to one of the wheat chromosomes. A relatively low frequency of the loci detected are from the D genome, and these loci show less polymorphism than those from the A and B genomes. Generally, the microsatellites show high levels of genetic polymorphism and an average of 3.5 alleles per locus with an average polymorphism information content (PIC), value of 0.51. The observed levels of polymorphism are positively correlated with the length of the microsatellite repeats. A high proportion, approximately two-thirds, of primer pairs designed to detect simple sequence repeat (SSR) variation in wheat do not generate the expected amplification products and, more significantly, often generate unresolvable PCR products. In general, our results agree closely with those obtained from other recent studies using microsatellites in plants.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/s001220050451

    Articles: DFG German National Licenses
  4. 4
    Staff View
    ISSN:
    1432-2242
    Keywords:
    Key words PCR markers ; Sequence-tagged-site ; Wheat
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Notes:
    Abstract  PCR products from regions corresponding to sequences hybridising to wheat RFLP probes were sequenced in order to establish the level of DNA sequence variation among adapted wheat genotypes. Hexaploid bread wheat shows a very low rate of nucleotide polymorphism, approximately 1 polymorphic nucleotide per 1000 basepairs. Differences in PCR product length can be exploited to design genome-specific amplicons, which may have use in gene tagging or in diagnostic applications. Interpretation of results may be complicated by the simultaneous amplification of orthologous and paralogous sequences. These findings have significant implications for the use of STS markers in wheat and other polyploid species.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/s001220051224

    Articles: DFG German National Licenses
  5. 5
    Staff View
    ISSN:
    1432-2242
    Keywords:
    Wheat ; Microsatellite markers
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Notes:
    Abstract In eukaryotes, tandem arrays of simple-sequence repeat sequences can find applications as highly variable and multi-allelic PCR-based genetic markers. In hexaploid bread wheat, a large-genome inbreeding species with low levels of RFLP, di- and trinucleotide tandem repeats were found in 22 published gene sequences, two of which were converted to PCR-based markers. These were shown to be genome-specific and displayed high levels of variation. These characteristics make them especially suitable for intervarietal breeding applications.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/BF00222209

    Articles: DFG German National Licenses
  6. 6
    Zhu, J. ; Gale, M. D. ; Quarrie, S. ; Jackson, M. T. ; Bryan, G. J.
    Springer
    Published 1998
    Staff View
    ISSN:
    1432-2242
    Keywords:
    Key words AFLP ; Rice ; Biodiversity ; Germplasm ; Mapping
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Notes:
    Abstract  AFLP was used as a DNA fingerprinting technique in rice (Oryza sativa L.) germplasm analysis. The high efficiency and random coverage of AFLP markers were established. With only five combinations of primers and RFLP anchors, a framework linkage map was constructed. This map demonstrated that the AFLP markers from a limited number of primers were not confined to any particular regions or chromosomes in the rice genome. To analyse the biodiversity of 57 rice germplasm accessions, we examined 179 polymorphic AFLP markers generated from four primer combinations. Both principal component analysis and cluster analysis were used, and three groups were clearly identified which corresponded to genotypes of Isozyme Groups I, II and VI. The number of markers needed for robust classification of rice germplasm and the diversity between/within the groups was established.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/s001220050778

    Articles: DFG German National Licenses
  7. 7
    Staff View
    ISSN:
    1432-2242
    Keywords:
    Key words Solanaceae ; Microsatellite ; Simple sequence repeat ; Chloroplast genome ; PCR-based markers
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Notes:
    Abstract  PCR-based markers were developed from mononucleotide simple-sequence repeats in the chloroplast genome of Nicotiana tabacum and applied to the analysis of genetic diversity. These markers were found to detect high levels of polymorphism at three taxonomic levels in Solanaceous plants. Of 36 chloroplast loci examined, 26 show some degree of polymorphism among potato accessions. Among a set of 30 tetraploid potato cultivars it is apparent that a single chloroplast haplotype is prevalent, presumably a result of the widespread use as a female parent of the imported US cultivar Rough Purple Chili in the latter half of the 19th century. Nonetheless, there is considerable chloroplast diversity in the cultivated potato, and it is clear that a large proportion of this variability has arisen through the use of wild or primitive cultivated species of potato in introgression programmes. This variability should be used in future breeding programmes. An examination of single accessions from 24 potato species, as well as representatives from tobacco and other members of the Solanaceae, reveals high levels of inter-specific chloroplast DNA variation. These data, and the ease of use and potential for multiplexing of these markers, suggest that cpSSRs will be of great utility in population genetics, germplasm management, evolutionary and phylogenetic studies as well as in, the analysis of material from introgression and somatic-fusion experiments. Interestingly, the polymorphism arising from one of the more-polymorphic chloroplast loci examined, does not originate solely from the SSR, and is due to variation in the copy number of two tandemly arrayed sequence elements.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/s001220051306

    Articles: DFG German National Licenses