Search Results - (Author, Cooperation:G. Giuliano)
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1F. Denoeud ; L. Carretero-Paulet ; A. Dereeper ; G. Droc ; R. Guyot ; M. Pietrella ; C. Zheng ; A. Alberti ; F. Anthony ; G. Aprea ; J. M. Aury ; P. Bento ; M. Bernard ; S. Bocs ; C. Campa ; A. Cenci ; M. C. Combes ; D. Crouzillat ; C. Da Silva ; L. Daddiego ; F. De Bellis ; S. Dussert ; O. Garsmeur ; T. Gayraud ; V. Guignon ; K. Jahn ; V. Jamilloux ; T. Joet ; K. Labadie ; T. Lan ; J. Leclercq ; M. Lepelley ; T. Leroy ; L. T. Li ; P. Librado ; L. Lopez ; A. Munoz ; B. Noel ; A. Pallavicini ; G. Perrotta ; V. Poncet ; D. Pot ; Priyono ; M. Rigoreau ; M. Rouard ; J. Rozas ; C. Tranchant-Dubreuil ; R. VanBuren ; Q. Zhang ; A. C. Andrade ; X. Argout ; B. Bertrand ; A. de Kochko ; G. Graziosi ; R. J. Henry ; Jayarama ; R. Ming ; C. Nagai ; S. Rounsley ; D. Sankoff ; G. Giuliano ; V. A. Albert ; P. Wincker ; P. Lashermes
American Association for the Advancement of Science (AAAS)
Published 2014Staff ViewPublication Date: 2014-09-06Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyComputer ScienceMedicineNatural Sciences in GeneralPhysicsKeywords: Caffeine/biosynthesis/*genetics ; Coffea/classification/*genetics ; *Evolution, Molecular ; *Genome, Plant ; Methyltransferases/genetics/*physiology ; Phylogeny ; Plant Proteins/genetics/*physiologyPublished by: -
2X. Xu ; S. Pan ; S. Cheng ; B. Zhang ; D. Mu ; P. Ni ; G. Zhang ; S. Yang ; R. Li ; J. Wang ; G. Orjeda ; F. Guzman ; M. Torres ; R. Lozano ; O. Ponce ; D. Martinez ; G. De la Cruz ; S. K. Chakrabarti ; V. U. Patil ; K. G. Skryabin ; B. B. Kuznetsov ; N. V. Ravin ; T. V. Kolganova ; A. V. Beletsky ; A. V. Mardanov ; A. Di Genova ; D. M. Bolser ; D. M. Martin ; G. Li ; Y. Yang ; H. Kuang ; Q. Hu ; X. Xiong ; G. J. Bishop ; B. Sagredo ; N. Mejia ; W. Zagorski ; R. Gromadka ; J. Gawor ; P. Szczesny ; S. Huang ; Z. Zhang ; C. Liang ; J. He ; Y. Li ; Y. He ; J. Xu ; Y. Zhang ; B. Xie ; Y. Du ; D. Qu ; M. Bonierbale ; M. Ghislain ; R. Herrera Mdel ; G. Giuliano ; M. Pietrella ; G. Perrotta ; P. Facella ; K. O'Brien ; S. E. Feingold ; L. E. Barreiro ; G. A. Massa ; L. Diambra ; B. R. Whitty ; B. Vaillancourt ; H. Lin ; A. N. Massa ; M. Geoffroy ; S. Lundback ; D. DellaPenna ; C. R. Buell ; S. K. Sharma ; D. F. Marshall ; R. Waugh ; G. J. Bryan ; M. Destefanis ; I. Nagy ; D. Milbourne ; S. J. Thomson ; M. Fiers ; J. M. Jacobs ; K. L. Nielsen ; M. Sonderkaer ; M. Iovene ; G. A. Torres ; J. Jiang ; R. E. Veilleux ; C. W. Bachem ; J. de Boer ; T. Borm ; B. Kloosterman ; H. van Eck ; E. Datema ; B. Hekkert ; A. Goverse ; R. C. van Ham ; R. G. Visser
Nature Publishing Group (NPG)
Published 2011Staff ViewPublication Date: 2011-07-12Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Evolution, Molecular ; Gene Duplication ; Gene Expression Regulation, Plant ; Genes, Plant/genetics ; Genetic Variation ; Genome, Plant/*genetics ; *Genomics ; Haplotypes/genetics ; Heterozygote ; Homozygote ; Immunity, Innate ; Inbreeding ; Molecular Sequence Annotation ; Molecular Sequence Data ; Plant Diseases/genetics ; Ploidies ; Solanum tuberosum/*genetics/physiologyPublished by: -
3Ventura, Mt ; Di Corato, R ; Giuliano, G ; Tursi, A
Copenhagen : Munksgaard International Publishers
Published 1999Staff ViewISSN: 1398-9995Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineType of Medium: Electronic ResourceURL: -
4Perrotta, G. ; Yahoubyan, G. ; Nebuloso, E. ; Renzi, L. ; Giuliano, G.
Oxford, UK : Blackwell Science, Ltd
Published 2001Staff ViewISSN: 1365-3040Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: BiologyNotes: The cryptochrome family of blue-light photoreceptors is involved in the control of plant photomorphogenesis and photoperiodic responses. Two cryptochromes have been described in Arabidopsis and tomato. To investigate the composition of the cryptochrome gene family in angiosperms, we used a ‘garden PCR’ approach, amplifying DNA from different plant species with the same pair of degenerated oligonucleotides representing conserved sequences from the flavin-binding domain. Different numbers of Cry-homologous sequences were found in different species: two each in Arabidopsis (Dicots, Brassicaceae), melon (Dicots, Cucurbitaceae) and banana tree (Monocots, Musaceae); three each in tomato (Dicots, Solanaceae) and barley (Monocots, Graminaceae). These sequences contain open reading frames (OFRs) with high homology to cryptochromes, but not photolyases, and are transcribed into RNA. In each case, a Cry1- and a Cry2-like sequence was recognizable. The third gene of tomato and barley seems to have arisen from recent, independent duplications of Cry1, and was thus named Cry1b. The tomato Cry1b gene encodes a protein of 583 amino acids (the shortest of the three tomato cryptochromes), with a high similarity to Cry1. The C-terminus of Cry1b is truncated before the conserved Ser-Thr-Ala-Glu-Ser-Ser-Ser (STAESSS) motif found in both Cry1a and Cry2. The Cry1b mRNA is expressed throughout the tomato plant, reaching maximal levels of expression in the flower (like Cry1a and Cry2). We conclude that tomato and barley contain at least one additional expressed member of the Cry1 gene family.Type of Medium: Electronic ResourceURL: -
5Ventura, M. ; Piccinni, T. ; Matino, M. G. ; Giuliano, G. ; Di Corato, R. ; Di Napoli, P. ; Tursi, A.
Copenhagen : Munksgaard International Publishers
Published 2001Staff ViewISSN: 1398-9995Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Background: In allergic rhinitis, allergenic stimulation causes the release of various mediators that induce symptoms and the development of chronic inflammation, which, in turn, is caused by cells involved in the late phase of inflammation, such as eosinophils. The eosinophils also cause damage at the mucosal level through the secretion of eosinophil cationic protein and other preformed factors contained in their granules. The objective was to verify the efficacy of fluticasone propionate aqueous nasal spray in patients with allergic rhinitis; in a retrospective study, we have evaluated mediators of inflammation, making correlations with the clinical symptoms score during and outside the pollen season. Methods: Forty patients with allergic rhinitis and 15 normal controls were included in our study. Eosinophil cationic protein, eosinophil chemotactic activity, and blood and nasal lavage eosinophil count were evaluated as laboratory parameters. Results: We found a significant increase in nasal lavage levels of eosinophil cationic protein in allergic patients, and this was strictly correlated with the clinical symptoms score. No differences were found in the eosinophil count of allergic patients and in the serum eosinophil cationic protein of patients sensitized to seasonal allergens in comparison with normal subjects. By contrast, an increase in serum eosinophil cationic protein level was found in patients sensitized to perennial allergens. After topical administration of fluticasone propionate aqueous nasal spray, a reduction in nasal lavage eosinophil cationic protein secretion was obtained with a reduction of eosinophil chemotactic activity at the local level. This reduction correlated with an improvement of clinical symptoms. Conclusions: The clinical improvement and reduction in nasal lavage eosinophil cationic protein and eosinophil chemotactic activity after administration of fluticasone propionate aqueous nasal spray further confirms the role of this treatment in allergic rhinitis.Type of Medium: Electronic ResourceURL: -
6Ventura, M. T. ; Di Corato, R. ; Dagnello, M. ; Giuliano, G. ; Tursi, A.
Copenhagen : Munksgaard International Publishers
Published 2000Staff ViewISSN: 1398-9995Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineType of Medium: Electronic ResourceURL: -
7Staff View
ISSN: 0166-0462Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: Architecture, Civil Engineering, SurveyingGeographySociologyEconomicsType of Medium: Electronic ResourceURL: -
8Santoni, G. ; Bavazzano, P. ; Perico, A. ; Colzi, A. ; Benassi, S. ; Medica, A. ; La Morgia, R. ; Giuliano, G.
Amsterdam : ElsevierStaff ViewISSN: 0378-4347Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: Chemistry and PharmacologyType of Medium: Electronic ResourceURL: -
9Staff View
ISSN: 0965-8564Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: Architecture, Civil Engineering, SurveyingType of Medium: Electronic ResourceURL: -
10Staff View
ISSN: 1572-8765Keywords: super plastic forming ; finite element methodSource: Springer Online Journal Archives 1860-2000Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision MechanicsNotes: Abstract FEM analysis has proved to be a powerful investigative tool capable of encompassing all the aspects that characterise an SPF process. However, despite the high potential of FEM programs they do not allow one to directly and suitably obtain the thickness of a sheet product for high deformation values, as commonly occurs in SPF processes. Many papers have been published on finite element analysis of S.P.F. process but the question of calculus accuracy in thicknesses of a sheet product has not been directly investigated. This problem has been already considered by the authors in a previous study which proposed an algorithm to determine thicknesses for a specific application. The software set up starts out with the results of the FEM modelling, keeps track of the “deformation” undergone by each element of the mesh and calculates to a good approximation the thicknesses at the end of the forming. Although the original version of the algorithm could only be used for the application studied an updated version is introduced in this study that can be used for any case. In other words, the software generates the thickness profile at the end of the analysis independently of technological set up, item shape and type of simulation (3D and 2D). The proposed algorithm was tested with reference to the superplastic forming of an item of simple geometry beginning with a thin circular plate blocked at the edges and put under constant hydrostatic pressure on one side. The test material, made superplastic by means of a series of repeated laminations, was characterised using an alternative method to the traditional tension test. The results of the experiments are in good accordance with the numerical predictions both in terms of thickness distribution and forming times.Type of Medium: Electronic ResourceURL: -
11Staff View
ISSN: 0168-9452Keywords: Daucus carota ; developmental mutant ; protoplast fusionSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyType of Medium: Electronic ResourceURL: -
12Staff View
ISSN: 0191-2607Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: Architecture, Civil Engineering, SurveyingType of Medium: Electronic ResourceURL: -
13Staff View
ISSN: 0191-2607Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: Architecture, Civil Engineering, SurveyingType of Medium: Electronic ResourceURL: -
14Staff View
ISSN: 0191-2607Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: Architecture, Civil Engineering, SurveyingType of Medium: Electronic ResourceURL: -
15Staff View
ISSN: 0191-2607Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: Architecture, Civil Engineering, SurveyingType of Medium: Electronic ResourceURL: -
16Staff View
ISSN: 0191-2615Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: Architecture, Civil Engineering, SurveyingEconomicsType of Medium: Electronic ResourceURL: -
17Boscolo, P. ; Di Gioacchino, M. ; Sabbioni, E. ; Benvenuti, F. ; Conti, P. ; Reale, M. ; Bavazzano, P. ; Giuliano, G.
Springer
Published 1999Staff ViewISSN: 1432-1246Keywords: Key words Immune system ; Atopy ; Lead ; Zinc ; Chromium ; NickelSource: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Abstract The objective of this study was to analyze the role of some trace metals in the immune system of non-allergic or atopic men. One of these elements (Zn) is essential for immune function, whereas others, present in the urban environment, are known to be allergenic (Ni and Cr) or toxic (Pb). Serum levels of interleukin (IL) 2, 4, 5, and 13 and of interferon-γ and immunoglobulins, blood lymphocyte subsets, blood concentrations of Pb and Zn, serum levels of Zn, and urinary Cr and Ni concentrations were determined in 17 nonallergic men (mean age 34 years) and 17 healthy nonsymptomatic atopic men living in urban areas. The mean blood concentration of Pb (a marker of exposure to toxic agents) was 11 μg/dl in both groups, which showed similar levels of blood Zn and of urinary Ni and Cr, whereas the serum Zn concentration was lower in the atopic group. Serum IgE levels were much higher in atopic men than in nonallergics, whereas serum IL-2, IL-5, and IL-13 concentrations were lower, possibly due to binding to tissue receptors and cells. Moreover, in atopic subjects, numbers of blood CD4+-CD45RO- “virgin” lymphocytes were significantly lower and the CD4+-CD45RO+/ CD4+-CD45RO- ratio was more elevated, indicating an activation of the immune system. Serum IgE levels of atopic men, in contrast to those of nonallergic subjects, were correlated with CD19+ and CD5- -CD19+ B lymphocytes. Blood Pb levels of both groups of men were correlated with CD4+, CD4+-CD45RO+, and HLA-DR+ [activated T-, B-, CO4+ -C. and natural killer (NK) cells] lymphocytes; in particular, blood Pb levels of the nonallergic men were also significantly correlated with CD25+ cells activated by IL-2, whereas those of the atopic men were also correlated with CD3- -HLA-DR+ (B- and NK-cells) and CD5- -CD19+ lymphocytes. Besides serum Zn levels, urinary Ni and Cr of nonallergic men were correlated with several immune parameters; in particular, urinary Cr was correlated with serum IL-5 and IgE and urinary Ni was correlated with CD4+-CD45RO+ and CD3+-CD25+ lymphocytes. This correlation of Ni and Cr, also found in previous studies in nonallergic subjects, confirms the hypothesis that these metals are involved in mechanisms of immune response regulation and that allergy to Ni or Cr represents an alteration of physiological mechanisms. Previous experimental studies have demonstrated that Pb exerts immunomodulatory effects on CD4+ and B- lymphocytes, enhancing the production of Th2-like cytokines and IgE. These experimental results confirm those of this study, showing in atopic men the correlation of B-lymphocytes with both blood Pb and serum IgE levels. This suggests that Pb may enhance the incidence of atopy in populations exposed to an urban environment.Type of Medium: Electronic ResourceURL: -
18Staff View
ISSN: 1432-203XSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract Nineteen chlorate-resistant variants were isolated after mutagenesis from cells of Medicago coerulea. The level of nitrate reductase activity was variable in these lines and ranged from 100% to less than 5% of the wild type level. Xanthine dehydrogenase was not affected in any of those variants tested. Methylammonium-resistant variants were also isolated from the same type of cells. They show a different regulation of nitrogen utilization. In particular, the enzymatic level of nitrate reductase which, in wild type cells, is sensitive to ammonium repression, is much less affected in the variants. Differences were also seen in the regulation of other functions of the nitrogen-utilizing pathway: xanthine dehydrogenase and, possibly, adenine uptake.Type of Medium: Electronic ResourceURL: -
19Staff View
ISSN: 1573-5028Keywords: stress ; differentiation ; plant cell cultures ; heat shockSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract The pattern of proteins synthesized by carrot cells after heat-shock was analyzed by SDS-polyacrylamide gel electrophoresis. A study was made of the time of appearance and disappearance of the heat-shock bands and a comparison was made of the heat-shock proteins present in cell extracts obtained from cell suspensions, callus, protoplasts and the different stages of somatic embryogenesis. From this comparison a number of differences and hence of stage-specific markers could be seen. This type of analysis was also carried out in the presence of α-amanitin in order to get information on the type of control of heat-shock protein synthesis. It turned out that in all stages after the initial globular stage, α-amanitin does not alter the heat-shock protein pattern suggesting pre-existence of the relative mRNA's.Type of Medium: Electronic ResourceURL: -
20Scolnik, P. A. ; Hinton, P. ; Greenblatt, I. M. ; Giuliano, G. ; Delanoy, M. R. ; Spector, D. L. ; Pollock, D.
Springer
Published 1987Staff ViewISSN: 1432-2048Keywords: Carotenoid biosynthesis ; Chloroplast in tomato mutant ; Chromoplast ; Lycopersicon (ghost mutant) ; Mutant (tomato) ; Tissue culture (of mutant)Source: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract The tomato (Lycopersicon esculentum (L.) Mill.) ghost plant is a mutant of the San Marzano cultivar affected in carotenoid biosynthesis. ghost plants exhibit a variable pattern of pigment biosynthesis during development. Cotyledons are green but true leaves are white. Green sectors, which appear to be clonal in origin, are frequently observed in the white tissue. Because of the lack of photosynthesis ghost plants have a very low viability in soil. We have developed a strategy for propagating ghost plants that employs organ culture to generate variegated green-white plants which, supported by the photosynthetic green areas, develop in soil to almost wild-type size. These plants were used to analyze the pigment content of the different tissues observed during development and plastid ultrastructure. Cotyledons and green leaves contain both colored carotenoids and chlorophyll but only the colorless carotenoid phytoene accumulates in white leaves. the plastids in the white tissue of ghost leaves lack internal membrane structures but normal chloroplasts can be observed in the green areas. The chromoplasts of white fruits are also impaired in their ability to form thylakoid membranes.Type of Medium: Electronic ResourceURL: