Search Results - (Author, Cooperation:G. Giovinazzo)
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1Latest Papers from Table of Contents or Articles in PressStaff View
Publication Date: 2013-07-12Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Animals ; Apoptosis ; Cell Proliferation ; Embryo, Mammalian/*cytology/*metabolism ; Embryonic Stem Cells/cytology/metabolism ; Female ; Gene Expression ; Genes, myc ; Germ Layers/*cytology/metabolism ; Male ; Mice ; Models, Biological ; Mosaicism/embryology ; Proto-Oncogene Proteins c-myc/*metabolismPublished by: -
2Articles: DFG German National LicensesStaff View
ISSN: 1432-203XKeywords: Key words Phaseolin ; Phaseolus vulgaris L. ; Protoplast ; Transient expressionSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract A suitable protocol for the transient expression of seed protein genes in protoplasts derived from cell suspension cultures of common bean has been established. Preliminary analyses of cultures to verify the synthesis of phaseolin – actively accumulated by the starting tissue, the developing cotyledon – showed that the protein was no longer synthesised after 5 days of culture. Transient expression of a phaseolin sequence, driven by a constitutive promoter, resulted in the accumulation of the correctly glycosylated and assembled protein. This system, when compared to tobacco protoplasts, largely avoids phaseolin fragmentation and the presence of contaminant polypeptides in the immunoprecipitates. Therefore, bean protoplasts are a good system to study the expression of wild-type as well as in-vitro-modified bean seed proteins.Type of Medium: Electronic ResourceURL: -
3Articles: DFG German National LicensesStaff View
ISSN: 1432-2242Keywords: Carrot ; Somatic embryogenesis ; Permanent dicentric system ; Chromosome variabilitySource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Summary A permanent dicentric chromosome system was studied on carrot cultures and regenerated somatic embryos at different stages of development. The large chromosomal variability of the cultures and the presence of the breakage-fusion bridge cycle did not interfere with the initial developmental process up to the seedling stage but subsequent growth proceeded only if healing of the broken ends or dicentric loss had occurred. The behaviour of the dicentric chromosome in culture and during somatic embryogenesis is discussed in relation to chromosomal variability, abnormal development and the somaclonal variation that such mechanisms may generate in regenerated plants.Type of Medium: Electronic ResourceURL: -
4Articles: DFG German National LicensesStaff View
ISSN: 1617-4623Source: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Summary We isolated a carrot cell mutant resistant to 8-azaguanine. Into this line a second mutation for resistance to α-amanitin was introduced. The double mutant was shown to be selectable in α-amanitin and counter-selectable in HAT medium. Resistance to Azg and sensitivity to HAT were correlated with an alteration of the enzyme HPRT. The properties of the double mutant as universal hybridizer were verified in a fusion with wild-type carrot protoplasts.Type of Medium: Electronic ResourceURL: