Search Results - (Author, Cooperation:G. E. Sosinsky)

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  1. 1
    Y. C. Yu ; S. He ; S. Chen ; Y. Fu ; K. N. Brown ; X. H. Yao ; J. Ma ; K. P. Gao ; G. E. Sosinsky ; K. Huang ; S. H. Shi
    Nature Publishing Group (NPG)
    Published 2012
    Staff View
    Publication Date:
    2012-06-09
    Publisher:
    Nature Publishing Group (NPG)
    Print ISSN:
    0028-0836
    Electronic ISSN:
    1476-4687
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Action Potentials/drug effects ; Animals ; Animals, Newborn ; *Cell Lineage ; *Electric Conductivity ; Electrical Synapses/metabolism/*physiology ; Gap Junctions/drug effects/*metabolism ; Meclofenamic Acid/pharmacology ; Mice ; Models, Neurological ; Neocortex/*cytology ; Neurons/*cytology/drug effects/*physiology ; Synaptic Transmission
    Published by:
    http://www.nature.com/

    Latest Papers from Table of Contents or Articles in Press
  2. 2
    Ghoshroy, S. ; Goodenough, D. A. ; Sosinsky, G. E.
    Springer
    Published 1995
    Staff View
    ISSN:
    1432-1424
    Keywords:
    Intercellular communication ; Membrane channel structure ; Image analysis ; Molecular recognition ; Cryoelectron microscopy
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Chemistry and Pharmacology
    Notes:
    Abstract Gap junctions, collections of membrane channels responsible for intercellular communication, contain two paired hemichannels (also called connexons). We have investigated conditions for splitting the membrane pair using urea. We have developed a protocol which consistently splits the gap junction samples with 60–90% efficiency. Our results indicate that hydrophobic forces are important in holding the two connexons together but that Ca2+ ions are also important in the assembly of the membrane pair. Greater yields and better structural integrity of split junctions were obtained with a starting preparation of gap junctions which had been detergent treated. Image analysis of edge views of single connexon layers reveal an asymmetry in the appearance of the cytoplasmic and extracellular surface. Cryo-electron microscopy and image analysis of split junctions show that the packing and structural detail of membranes containing arrays of single connexons are the same as for intact junctions, and that the urea treatment causes no gross structural changes in the connexon assembly.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/BF00232677

    Articles: DFG German National Licenses