Search Results - (Author, Cooperation:F. Grassi)
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1Latest Papers from Table of Contents or Articles in PressD. Di Mitri ; A. Toso ; J. J. Chen ; M. Sarti ; S. Pinton ; T. R. Jost ; R. D'Antuono ; E. Montani ; R. Garcia-Escudero ; I. Guccini ; S. Da Silva-Alvarez ; M. Collado ; M. Eisenberger ; Z. Zhang ; C. Catapano ; F. Grassi ; A. Alimonti
Nature Publishing Group (NPG)
Published 2014Staff ViewPublication Date: 2014-08-27Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Animals ; *Cell Aging/drug effects ; *Cell Movement ; Disease Progression ; Drug Resistance, Neoplasm ; Humans ; Immunity, Innate ; Interleukin 1 Receptor Antagonist Protein/deficiency/metabolism/secretion ; Interleukin-1alpha/immunology/metabolism ; Male ; Mice ; Myeloid Cells/*cytology/*metabolism/transplantation ; PTEN Phosphohydrolase/deficiency/genetics/metabolism ; Prostatic Neoplasms/drug therapy/immunology/metabolism/*pathology ; Receptors, Chemokine/*metabolism ; Receptors, Interleukin-8B/antagonists & inhibitors ; Taxoids/pharmacology ; Tumor Escape ; Tumor MicroenvironmentPublished by: -
2Articles: DFG German National LicensesImazio, S. ; Labra, M. ; Grassi, F. ; Winfield, M. ; Bardini, M. ; Scienza, A.
Oxford, UK : Blackwell Publishing Ltd
Published 2002Staff ViewISSN: 1439-0523Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: In viticulture, biotype identification problems have traditionally been solved using ampelography, ampelometry and chemical traits analysis. However, these tools have resulted in several false attributions, in particular when used at the clonal level. The availability of relatively cheap, reliable and reproducible tools to identify genetic differences at the clonal level would greatly facilitate the work of clonal patenting. In this work, 24 accessions of ‘Traminer’ cultivars were characterized using molecular markers. Three different approaches were applied: simple sequence repeats (SSR), amplified fragment length polymorphism (AFLP) and methyl-sensitive amplified length polymorphism (MSAP). Results showed that SSRs were not a powerful tool for clonal distinction. In contrast, the AFLP technique was able to distinguish 16 out of the 24 cultivars, even though the average similarity was high (97.1%). The MSAP technique was used to evaluate qualitative differences in the degree of DNA methylation among clones. Results suggest that morphological differences among clones are probably due to the synergetic effect of genetic and epigenetic modifications, and that clonal identification could be greatly improved using molecular tools such as AFLP and MSAP.Type of Medium: Electronic ResourceURL: -
3Articles: DFG German National LicensesLABRA, M. ; IMAZIO, S. ; GRASSI, F. ; ROSSONI, M. ; SALA, F.
Oxford, UK : Blackwell Publishing Ltd
Published 2004Staff ViewISSN: 1439-0523Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: The sequence-specific amplification polymorphism (S-SAP) method, derived from the amplified fragment length polymorphism (AFLP) technique, produces amplified fragments containing retrotransposon long terminal repeat (LTR) sequence at one end and a host restriction site at the other. The development and application of this procedure to the LTR of the Vine-1 element from grapevine is reported. Two primers derived from one of the LTR sequences flanking the retrotransposon were used in combination with MseI degenerated primers on 15 grapevine accessions. S-SAP results were compared with AFLP data. The heterozygosity and gene diversity values were higher for S-SAP than for the AFLP procedure. Results show that S-SAP amplification is effective in identifying polymorphisms and defining genetic distances among cultivars, and could be used for fingerprinting and for ‘Traminer’ clone identification. To the contrary Vine-1 retrotransposon-based S-SAP was not able to distinguish ‘Pinot’ clones.Type of Medium: Electronic ResourceURL: -
4Articles: DFG German National LicensesRagozzino, D. ; Fucile, S. ; Giovannelli, A. ; Grassi, F. ; Mileo, A. M. ; Ballivet, M. ; Alemà, S. ; Eusebi, F.
Oxford, UK : Blackwell Publishing Ltd
Published 1997Staff ViewISSN: 1460-9568Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: To study how subunit composition affects the functional properties of neuronal nicotinic acetylcholine receptors (nAChRs), we examined the behaviour of acetylcholine (ACh)-induced single-channel currents in human BOSC 23 cells transiently transfected with various subunit cDNA combinations. For all nAChRs examined (chick and rat α3β4, chick α〈3/β2, α4β2, α4β4, α7and α8, expression levels were high enough to allow measurements of acetylcholine-evoked whole-cell currents and nicotine-elicited Ca2+ transients as well as the functional characterization of nAChR channels. Unitary acetylcholine-evoked events of α8 nAChR had a slope conductance of 23 pS, whereas two conductance classes (19–23 and 32–45 pS) were identified for all other nAChR channels. The mean channel open times were significantly longer for homomeric α7 and α8 nAChRs (6–7 ms) than for heteromeric nAChRs (1–3 ms), with the exception of α3α4nAChRs (8.4 ms for rat, 7 ms for chick). At least two species of heterologously expressed nAChRs (α3α4and α3α2) exhibited single-channel characteristics similar to those reported for native receptors. The variety of nAChRs channel conductance and kinetic properties encountered in human cells transfected with nAChR subunits contributes to the functional diversity of nAChRs in nerve cells.Type of Medium: Electronic ResourceURL: -
5Articles: DFG German National LicensesStaff View
ISSN: 0006-291XSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyPhysicsType of Medium: Electronic ResourceURL: -
6Articles: DFG German National LicensesStaff View
ISSN: 0920-5632Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: PhysicsType of Medium: Electronic ResourceURL: -
7Articles: DFG German National LicensesStaff View
ISSN: 0898-6568Keywords: Acetylcholine receptor ; desensitization ; myotube ; v-ras oncogene ; v-src oncogeneSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyMedicineType of Medium: Electronic ResourceURL: -
8Articles: DFG German National LicensesGiovannelli, A. ; Limatola, C. ; Mattei, E. ; Grassi, F. ; Ragozzino, D. ; Eusebi, F.
Amsterdam : ElsevierStaff ViewISSN: 0928-4257Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyMedicineType of Medium: Electronic ResourceURL: -
9Articles: DFG German National LicensesStaff View
ISSN: 0014-4827Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyMedicineType of Medium: Electronic ResourceURL: -
10Articles: DFG German National LicensesStaff View
ISSN: 0012-1606Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyType of Medium: Electronic ResourceURL: -
11Articles: DFG German National LicensesStaff View
ISSN: 0006-291XSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyPhysicsType of Medium: Electronic ResourceURL: -
12Articles: DFG German National LicensesStaff View
ISSN: 0006-291XSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyPhysicsType of Medium: Electronic ResourceURL: -
13Articles: DFG German National LicensesStaff View
ISSN: 0370-2693Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: PhysicsType of Medium: Electronic ResourceURL: -
14Articles: DFG German National LicensesStaff View
ISSN: 0375-9474Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: PhysicsType of Medium: Electronic ResourceURL: -
15Articles: DFG German National LicensesStaff View
ISSN: 0375-9474Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: PhysicsType of Medium: Electronic ResourceURL: -
16Articles: DFG German National LicensesStaff View
ISSN: 0375-9601Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: PhysicsType of Medium: Electronic ResourceURL: -
17Articles: DFG German National LicensesGrassi, F. ; Monaco, L. ; Fratamico, G. ; Dolci, S. ; Iannini, E. ; Conti, M. ; Eusebi, F. ; Stefanini, M.
Amsterdam : ElsevierStaff ViewISSN: 0309-1651Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyType of Medium: Electronic ResourceURL: -
18Articles: DFG German National LicensesStaff View
ISSN: 1432-1912Keywords: TPA ; Protein kinase C ; Transmitter release ; Neuromuscular junctionSource: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Summary 1. The effect of the phorbol ester 12-O-tetradecanoylphorpbol-13-acetate (TPA) on the stimulation-evoked neurotransmitter release has been investigated by measuring the quantal content (m) of end-plate potentials at frog neuromuscular junctions (Rana temporaria, M. sartorius). 2. After addition of TPA (0.1 up to 1 μmol/1) to the Ringer solution the m-values increased in a concentration-dependent manner up to more than 3 times the control values. 3. Inhibition of the activity of the protein kinase C through the inhibitor 1(5-isoquinolinylsulfonyl)-2-methylpiperazine (H-7) blocked this effect of TPA. 4. The TPA effect was much more conspicuous when the m-value was reduced by raising the extracellular Mg2+ concentration. Between the control m-values and the n-fold increase in the m-value enhanced by TPA a hyperbolic relation was observed. 5. It is concluded that protein kinase C stimulation affects predominantly the spontaneous release of neurotransmitter at the frog neuromuscular junction and only very poorly the stimulation-evoked one.Type of Medium: Electronic ResourceURL: -
19Articles: DFG German National LicensesStaff View
ISSN: 1432-2013Keywords: Key words Adult acetylcholine receptor ; Fetal acetylcholine receptor ; 5-Hydroxytryptamine ; Mouse muscle ; Open channel block ; Patch-clampSource: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Abstract The action of 5-hydroxytryptamine (5-HT) on fetal (γ-AChR) and adult (ɛ-AChR) muscle acetylcholine receptors was studied in transfected BOSC 23 cells expressing mouse AChRs and in acutely dissociated mouse muscle fibres. In transfected cells, coapplication of 5-HT (0.01–10 mM) with ACh reversibly reduced the amplitude and accelerated the decay of the ACh-activated whole-cell currents, in a dose- and voltage-dependent manner. 5-HT blocked faster and with higher potency the γ-AChR than the ɛ-AChR. Cell-attached recordings from transfected BOSC 23 cells and embryonic or neonatal muscle fibres were made. When 5-HT (5 µM) was included in the patch pipette, ACh-evoked unitary events acquired a bursting behaviour, which was more pronounced for γ- than ɛ-AChR, though it was enhanced by membrane hyperpolarization for both AChR species. There was no effect on the single-channel conductance. It is concluded that 5-HT behaves as an open-channel blocker of muscle AChRs, with higher efficacy on γ- than on ɛ-AChR.Type of Medium: Electronic ResourceURL: -
20Articles: DFG German National LicensesStaff View
ISSN: 1432-2013Keywords: Calcium ; Acetylcholine ; Nicotine ; Nicotinic acetylcholine receptor ; C2C12 myotubes ; Inositol 1,4,5-trisphosphateSource: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Abstract In cultured mouse C2C12 myotubes, digital Ca2+ imaging fluorescence microscopy using the acetoxymethyl ester of Fura-2, Fura-2-AM, showed that, in the absence of extracellular Ca2+, acetylcholine (ACh) and nicotine, but not muscarine, raised the intracellular concentration of Ca2+ ([Ca2+]i) by about tenfold. AChinduced Ca2+ mobilization was prevented by thapsigargin, a drug known to deplete inositol 1,4,5-trisphosphate (InsP3)-sensitive stores, and was concomitant with InsP3 accumulation. Caffeine, which releases Ca2+ from the ryanodine-sensitive stores of the sarcoplasmic reticulum, did not interfere with the ACh-induced [Ca2+]i increase. Ca2+ mobilization was also inhibited when myotubes were depolarized by high K+, or when extracellular Na+ was omitted. Nicotinic ACh receptor (nAChR) stimulation lowered intracellular pH with a time course slower than the [Ca2+]i increase. Possible mechanisms linking the current flowing through the nAChR pore to [Ca2+]i increase are discussed.Type of Medium: Electronic ResourceURL: