Search Results - (Author, Cooperation:F. G. Grosveld)

2015-06-25

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Alternative Splicing/physiology ; Ataxia Telangiectasia Mutated Proteins/*metabolism ; Cell Line ; Chromatin/metabolism ; DNA Damage/*physiology ; DNA-Directed RNA Polymerases/metabolism ; Enzyme Activation ; Humans ; *Signal Transduction ; Spliceosomes/*metabolism ; Ultraviolet Rays

1744-313X

Blackwell Publishing Journal Backfiles 1879-2005

Biology

Medicine

K36.16 is an AKR H-2k thymoma which expresses an aberrant H-2Dd-like allospecificity, does not have a detectable amount of the H-2Kk syngeneic antigen and grows very easily in syngeneic mice. By DNA-mediated gene transfer experiments, we were able to obtain transformed clones which do express the H-2Kk molecules and are rejected by AKR mice. Southern hybridization was performed to assess whether any gross changes had occurred in the K36.16 H-2K locus or elsewhere in the MHC, which might explain the lack of H-2K expression and/or the presence of the aberrant H-2Dd-like allospecificity. Specific H-2 class I DNA probes were used to compare the K36.16 genomic DNA with normal AKR thymus DNA after digestion with a variety of restriction enzymes. After hybridization with the pH-2IIa probe a 2.8 kb ‘Hind III’ fragment was identified in the K36.16 genomic DNA which is absent from AKR DNA. The pH-2IIa probe detects the third, transmembrane and cytoplasmic domains of class I genes. Although these changes are indicative of MHC genome modifications it is not yet possible to link these specific Southern blot pattern variations with the phenotypic changes mentioned above.

Electronic Resource

1744-313X

Blackwell Publishing Journal Backfiles 1879-2005

Biology

Medicine

By the isolation of overlapping cosmid clones and ‘chromosome walking’ studies from the H-2Kk gene, we have obtained cosmid clones encoding the H-2K1k gene from two separate cosmid libraries. The nucleotide sequence of one of the clones was determined. The cloned H-2K1k gene could be transcribed in vitro to give a normal H-2 class I mRNA of 1.7 kb. However, the deletion of four nucleotides in exon 3 of the H-2K1k gene results in a translation termination codon at the beginning of exon 4. In agreement with this, when expressed in human cells, the H-2K1k gene gave a truncated, cytoplasmic polypeptide of Mr 36,000. Therefore, although the H-2K1k gene is homologous to other class I MHC genes in its molecular organization and nucleotide sequence, it is a pseudogene. When compared to the nucleotide sequence of the H-2Kk gene, the H-2K1k gene has undergone many substitutions of methylated CpG residues (meCpG). This represents further evidence to suggest that this gene is inactive.

Electronic Resource

1476-4687

Nature Archives 1869 - 2009

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

[Auszug] The β-globin gene present on the deletion locus in a Dutch γβ-thalassaemic patient was found to be identical to the normal β-globin gene with respect to DNA sequence and its transcription in HeLa cells. DNase I sensitivity and methylation experiments show that the affected ...

Electronic Resource

1476-4687

Nature Archives 1869 - 2009

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

[Auszug] Cosmids containing regions of the human -globin gene cluster (see Fig. 1) were introduced into thymidine kinase-negative (tk) MEL cells6 by calcium phosphate transformation9. Before transformation, DNA was linearized within the vector sequences by cleavage with Pvul. Stable tk+ transformants were ...

Electronic Resource