Search Results - (Author, Cooperation:E. Ficker)

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  1. 1
    Staff View
    Publication Date:
    2012-03-01
    Publisher:
    Nature Publishing Group (NPG)
    Print ISSN:
    0028-0836
    Electronic ISSN:
    1476-4687
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Animals ; Arrhythmias, Cardiac/complications/genetics/*physiopathology ; Cells, Cultured ; Circadian Rhythm/genetics/*physiology ; DNA-Binding Proteins/deficiency/genetics/metabolism ; Death, Sudden, Cardiac/etiology ; Electrocardiography ; Gene Expression Regulation ; Heart Conduction System/*physiology ; Heart Rate/physiology ; Heart Ventricles/cytology ; Kv Channel-Interacting Proteins/biosynthesis/genetics ; Male ; Mice ; Mice, Inbred C57BL ; Muscle Cells/cytology ; Promoter Regions, Genetic/genetics ; Rats ; Time Factors ; Transcription Factors/deficiency/genetics/metabolism
    Published by:
    http://www.nature.com/

    Latest Papers from Table of Contents or Articles in Press
  2. 2
    Eder, C. ; Ficker, E. ; Gündel, J. ; Heinemann, U.

    Oxford, UK : Blackwell Publishing Ltd
    Published 1991
    Staff View
    ISSN:
    1460-9568
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Medicine
    Notes:
    We have studied outward currents of neurons acutely isolated from superficial layers of the entorhinal cortex with whole-cell patch-clamp recordings. If cells were held more negative than -50 mV, depolarizing voltage commands activated a transient A-type current together with a sustained outward current. Both currents were sensitive to 4-aminopyridine, while only the sustained current was blocked by tetraethylammonium. The sustained outward current showed a considerable rundown in amplitude over prolonged recording periods. At the same time its half-maximal inactivation shifted from -74 to - 114 mV. Nystatin perforated patch recordings were used to minimize these perfusion effects. Under such conditions the amplitude and the steady-state inactivation properties of the sustained outward current remained stable for more than 1 h. Pharmacological investigations revealed that only a small part of the sustained outward current could be attributed to a calcium-activated potassium current. Therefore most of the rundown has to be due to changes in the delayed rectifier outward current. These results may suggest that the delayed rectifier current is under considerable metabolic control.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1111/j.1460-9568.1991.tb00060.x

    Articles: DFG German National Licenses
  3. 3
    Glimm, H. ; Ficker, E. ; Heinemann, U.
    Springer
    Published 1996
    Staff View
    ISSN:
    1432-1106
    Keywords:
    Dentate gyrus culture ; Non-granule cells ; High-voltage-activated Ca2+ current ; Delayed rectifier K+ current ; Ca2+-dependent K+ current
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Medicine
    Notes:
    Abstract Electrophysiological properties of neurofilament-positive neurones in dissociated cell cultures were prepared at postnatal days 4–5 from rat dentate gyrus and studied using the whole-cell patch-clamp technique. These cells expressed a fast-inactivating, 0.5 μM tetrodotoxin-sensitive Na+ current; a high-voltage-activated (HVA) Ca2+ current, which was 30 μM Cd2+- and partially 2 μM nicardipine-sensitive; and an inward rectifier current, which was sensitive to extracellularly applied 1 mM Cs+. The outward current pattern was composed of a delayed rectifier-like outward current sensitive to 20 mM tetraethylammonium (TEA) and a fast-inactivating, Ca2+-dependent outward current. This transient Ca2+-dependent K+ outward current was identified by a subtraction procedure. K+ currents recorded under conditions of blocked Ca2+ currents (after rundown of the HVA Ca2+ current or blocked by extracellularly applied Cd2+) were subtracted from control currents. By comparison with the current pattern of identified dentate granule cells, it is concluded that the investigated cell type originated from interneurones or projection neurones of the dentate hilus.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/BF00230419

    Articles: DFG German National Licenses
  4. 4
    Ficker, E. ; Heimemann, U.
    Springer
    Published 1989
    Staff View
    ISSN:
    1432-2013
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Medicine
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/BF00582262

    Articles: DFG German National Licenses