Search Results - (Author, Cooperation:E. Bidnenko)

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  1. 1
    Staff View
    Publication Date:
    2012-03-03
    Publisher:
    American Association for the Advancement of Science (AAAS)
    Print ISSN:
    0036-8075
    Electronic ISSN:
    1095-9203
    Topics:
    Biology
    Chemistry and Pharmacology
    Computer Science
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    *Adaptation, Physiological ; Algorithms ; Bacillus subtilis/*genetics/*metabolism ; Bacterial Proteins/metabolism ; Computer Simulation ; Data Interpretation, Statistical ; Gene Expression Regulation, Bacterial ; *Gene Regulatory Networks ; Genome, Bacterial ; Glucose/*metabolism ; Malates/*metabolism ; Metabolic Networks and Pathways/*genetics ; Metabolome ; Metabolomics ; Models, Biological ; Operon ; Promoter Regions, Genetic ; Transcription Factors/metabolism ; Transcription, Genetic
    Published by:
    Latest Papers from Table of Contents or Articles in Press
  2. 2
    Staff View
    Publication Date:
    2012-03-03
    Publisher:
    American Association for the Advancement of Science (AAAS)
    Print ISSN:
    0036-8075
    Electronic ISSN:
    1095-9203
    Topics:
    Biology
    Chemistry and Pharmacology
    Computer Science
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Adaptation, Physiological ; Algorithms ; Bacillus subtilis/*genetics/*physiology ; Binding Sites ; Gene Expression Profiling ; *Gene Expression Regulation, Bacterial ; Gene Regulatory Networks ; Oligonucleotide Array Sequence Analysis ; *Promoter Regions, Genetic ; RNA, Antisense/genetics/metabolism ; RNA, Bacterial/genetics/metabolism ; RNA, Messenger/genetics/metabolism ; Regulon ; Sigma Factor/metabolism ; Terminator Regions, Genetic ; *Transcription, Genetic ; *Transcriptome
    Published by:
    Latest Papers from Table of Contents or Articles in Press
  3. 3
    Bidnenko, E. M. ; Akhverdyan, V. Z. ; Krylov, V. N.
    Springer
    Published 2000
    Staff View
    ISSN:
    1608-3369
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Notes:
    Abstract Regulation of transcription was studied in a wild-type transposable phage (TP) D3112 of Pseudomonas aeruginosaand its mutants for different genes. For this purpose, [3H]RNA–DNA hybridization with denatured fragments from various regions of the D3112 genome bound to nitrocellulose filters was used. A transcriptional map of TP D3112 was constructed based on the data obtained. The map comprised six independent transcriptional units corresponding to the modular organization of the phage genome. Only the repressor gene cI was transcribed in the lysogenic state. After repressor thermoinactivation, the cI transcription ceased, and transcription occurred in the same order as the genes (modules) were located on the D3112 phage genetic map (from left to right): cip1(a negative regulator) and early genes A and B (controlling the replication–transposition of the phage), nonessential genome region, genes C and the locus marked with the ts47 mutation (positive regulators of late gene transcription), the genes of the head morphogenesis, gene c91(a positive regulator responsible for the lysogenic state), and the genes of the tail morphogenesis. Similarities between nonhomologous TPs D3112 of P. aeruginosa and Mu of Escherichia coli with respect to genetic organization and transcription regulation are discussed.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  4. 4
    Bidnenko, E. M. ; Akhverdyan, V. Z. ; Krylov, V. N.
    Springer
    Published 2000
    Staff View
    ISSN:
    1608-3369
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Notes:
    Abstract At nonpermissive temperature (42°C) thets47 mutation causes substantial abnormalities in the late phase of the phage intracellular development. In these conditions DNA of the D3112 phage is detected both in a free form and integrated into bacterial chromosome. The transcription kinetics in the ts47 mutant at 42°C was indistinguishable from that typical to other early gene mutants (A, B, and C): specifically, the preservation of the first transcription peak along with low activity of late transcription were observed. Similarly to the C gene, the ts47 mutation-carrying locus is involved in regulating the transcription of the D3112 transposable phage late genes. It is suggested that the mechanism underlying the action of thets47 mutation differs from that of the C gene product. One of the possible explanations is based on the fact that the product of the ts47 locus affects the activity of cellular RNA polymerase via providing more effective recognition of the phage promoters by the RNA polymerase modified with the phage protein C.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses