Search Results - (Author, Cooperation:D. Pot)
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1F. Denoeud ; L. Carretero-Paulet ; A. Dereeper ; G. Droc ; R. Guyot ; M. Pietrella ; C. Zheng ; A. Alberti ; F. Anthony ; G. Aprea ; J. M. Aury ; P. Bento ; M. Bernard ; S. Bocs ; C. Campa ; A. Cenci ; M. C. Combes ; D. Crouzillat ; C. Da Silva ; L. Daddiego ; F. De Bellis ; S. Dussert ; O. Garsmeur ; T. Gayraud ; V. Guignon ; K. Jahn ; V. Jamilloux ; T. Joet ; K. Labadie ; T. Lan ; J. Leclercq ; M. Lepelley ; T. Leroy ; L. T. Li ; P. Librado ; L. Lopez ; A. Munoz ; B. Noel ; A. Pallavicini ; G. Perrotta ; V. Poncet ; D. Pot ; Priyono ; M. Rigoreau ; M. Rouard ; J. Rozas ; C. Tranchant-Dubreuil ; R. VanBuren ; Q. Zhang ; A. C. Andrade ; X. Argout ; B. Bertrand ; A. de Kochko ; G. Graziosi ; R. J. Henry ; Jayarama ; R. Ming ; C. Nagai ; S. Rounsley ; D. Sankoff ; G. Giuliano ; V. A. Albert ; P. Wincker ; P. Lashermes
American Association for the Advancement of Science (AAAS)
Published 2014Staff ViewPublication Date: 2014-09-06Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyComputer ScienceMedicineNatural Sciences in GeneralPhysicsKeywords: Caffeine/biosynthesis/*genetics ; Coffea/classification/*genetics ; *Evolution, Molecular ; *Genome, Plant ; Methyltransferases/genetics/*physiology ; Phylogeny ; Plant Proteins/genetics/*physiologyPublished by: -
2Brendel, O. ; Pot, D. ; Plomion, C. ; Rozenberg, P. ; Guehl, J.-M.
Oxford, UK : Blackwell Science Ltd
Published 2002Staff ViewISSN: 1365-3040Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: BiologyNotes: Classical quantitative genetics and quantitative trait dissection analysis (QTL) approaches were used in order to investigate the genetic determinism of wood cellulose carbon isotope composition (δ13C, a time integrated estimate of water use efficiency) and of diameter growth and their relationship on adult trees (15 years) of a forest tree species (maritime pine). A half diallel experimental set-up was used to (1) estimate heritabilities for δ13C and ring width and (2) to decompose the phenotypic δ13C/growth correlation into its genetic and environmental components. Considerable variation was found for δ13C (range of over 3‰) and for ring width (range of over 5 mm) and significant heritabilities (narrow sense 0·17/0·19 for δ13C and ring width, respectively, 100% additivity). The significant phenotypic correlation between δ13C and ring width was not determined by the genetic component, but was attributable to environmental components. Using a genetic linkage map of a full-sib family, four significant and four suggestive QTLs were detected for δ13C, the first for δ13C in a forest tree species, as far as known to the authors. Two significant and four suggestive QTLs were found for ring width. No co-location of QTLs was found between δ13C and growth.Type of Medium: Electronic ResourceURL: -
3CASASOLI, M ; POT, D. ; PLOMION, C. ; MONTEVERDI, M. C. ; BARRENECHE, T. ; LAUTERI, M. ; VILLANI, F.
Oxford, UK : Blackwell Science Ltd
Published 2004Staff ViewISSN: 1365-3040Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: BiologyNotes: A QTL analysis for three different adaptive traits was performed in an F1 progeny of Castanea sativa Mill. The female and male parents originated from two Turkish chestnut populations adapted to a drought and humid environment, respectively. QTLs for bud flush, growth and carbon isotope discrimination were detected over a 3-year period. Bud set was also recorded in the last year of measurement. Thirty-five individual QTLs were detected for phenology, 28 for growth and 17 for carbon isotope discrimination, most of them explaining a low to moderate proportion of the total phenotypic variance. QTLs were distributed throughout the whole genome. Temporally stable QTLs were identified for all the traits analysed, with phenology showing the higher proportion of stable QTLs. Interesting phenotypic correlations and co-localizations among QTLs for different adaptive traits were observed, allowing the formulation of an hypothesis about the genetic adaptation of the female parent to drought.Type of Medium: Electronic ResourceURL: -
4Costa, P. ; Pot, D. ; Dubos, C. ; Frigerio, J. M. ; Pionneau, C. ; Bodenes, C. ; Bertocchi, E. ; Cervera, M. -T. ; Remington, D. L. ; Plomion, C.
Springer
Published 2000Staff ViewISSN: 1432-2242Keywords: Key words Pinus pinaster ; AFLP ; RAPD ; Protein ; Linkage map ; QTLSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract TheAFLP (amplified fragment length polymorphism) technique was adapted to carry out genetic analysis in maritime pine, a species characterized by a large genome size (24 pg/C). A genetic linkage map was constructed for one F1 individual based on 239 AFLP and 127 RAPD (randomly amplified polymorphic DNA) markers. Markers were scored on megagametophytes (1n) from 200 germinated F2 seedlings. Polymorphism rate, labour time and cost of both AFLP and RAPD techniques were compared. The AFLP technique was found to be twice as fast and three-times less costly per marker than the RAPD technique. Thirteen linkage groups were identified with a LOD score ≥6 covering 1873 cM, which provided 93.4% of genome coverage. Proteins were extracted from needles (2n) of the F2 progeny and revealed by 2-DE (two-dimensional electrophoresis). Thirty one segregating proteins were mapped using a QTL detection strategy based on the quantification of protein accumulation. Two framework maps of the same F1 individual are now available. The first map (Plomion et al. 1996) uses RAPD markers and the second map, presented in this study, uses mostly AFLP markers. Although the total genetic length of both maps was almost identical, differences among homologous groups were observed.Type of Medium: Electronic ResourceURL: -
5Plomion, C. ; Hurme, P. ; Frigerio, J-M. ; Ridolfi, M. ; Pot, D. ; Pionneau, C. ; Avila, C. ; Gallardo, F. ; David, H. ; Neutelings, G. ; Campbell, M. ; Canovas, F.M. ; Savolainen, O. ; Bodénès, C. ; Kremer, A.
Springer
Published 1999Staff ViewISSN: 1572-9788Keywords: SSCP ; Pinus pinaster ; Pinus sylvestris ; linkage mapSource: Springer Online Journal Archives 1860-2000Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: Abstract This study demonstrates the feasibility of generating sequence- based markers in Pinus species, from data available in electronic databases. Nucleotide sequences from 23 partially or fully characterized cDNAs or genomic sequences of pines were used to design PCR primers for amplifying targeted fragments of genomic DNA from Maritime and Scots pine. Various template DNA and MgCl2 concentrations, annealing temperatures, and buffer compositions were used to optimize the PCR amplifications. The polymorphism of 16 sequences was then investigated in a tree-generation inbred pedigree of Maritime pine and in a two-generation pedigree of Scots pine, using single-stranded DNA conformation polymorphism (SSCP) on polymerase chain reaction (PCR) products. The level of polymorphism was shown to be independent of (1) fragment size, (2) the presence or absence of introns in the amplified product and (3) temperature during electrophoresis. Mendelian segregation was tested for 5 SSCP markers in each species. Chromosomal locations of five genes were identified by linkage analysis with previously mapped markers in a genetic map of Maritime pine. The use of SSCP is recommended for constructing a transcriptional map for comparative mapping studies among pines and to provide useful ‘candidate genes’ for characterizing quantitative trait loci.Type of Medium: Electronic ResourceURL: