Search Results - (Author, Cooperation:D. Monack)

2012-08-14

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Amino Acid Sequence ; Animals ; CARD Signaling Adaptor Proteins/chemistry/deficiency/genetics/*metabolism ; Calcium-Binding Proteins/chemistry/deficiency/genetics/*metabolism ; Caspase 1/metabolism ; Enzyme Activation ; Gene Knock-In Techniques ; Humans ; Immunity, Innate/immunology ; Inflammasomes/*metabolism ; Interleukin-1beta/immunology/secretion ; Macrophages/immunology ; Mice ; Molecular Sequence Data ; Phosphorylation ; Protein Conformation ; Protein Kinase C-delta/deficiency/genetics/metabolism ; Salmonella typhimurium/immunology ; Sequence Alignment

2018-06-08

American Association for the Advancement of Science (AAAS)

0036-8075

1095-9203

Biology

Chemistry and Pharmacology

Geosciences

Computer Science

Medicine

Natural Sciences in General

Physics

1365-2958

Blackwell Publishing Journal Backfiles 1879-2005

Biology

Medicine

The bvg locus contains two genes, bvgA and bvgS, which control the expression of the virulence-associated genes in Bordetella species by a system similar to the two-component systems used by a variety of bacterial species to respond to environmental stimuli. We determined the nucleotide sequence of the bvg loci of Bordetella parapertussis and Bordetella bronchiseptica and compared them with the previously determined sequence of Bordetella pertussis. The nucleotide and amino acid sequences of the bvg loci of these species are well conserved in those regions coding for the protein domains which have putative kinase and DNA-binding activities. In marked contrast, the region of BvgS that codes for the protein domain with putative sensor activity shows a high degree of variability. In total, we find 198 base-pair changes in the bvg loci of B. parapertussis and B. bronchiseptica relative to the bvg locus of B. pertussis. One hundred and seventy-three of these base-pair changes are identical in S. parapertussis and B. bronchiseptica. Tills confirms our previous observation that B. parapertussis and B. bronchiseptica are more related to each other than to B. pertussis. We have mapped the mutations that cause phase changes in B. bronchiseptica and we have found that in three cases these are due to spontaneous deletions in the bvgS gene.The wild-type bvg locus present on a multicopy plasmid cannot complement avirulent derivatives of B. bronchiseptica to wild-type levels, but it can do so when the bvgA gene on the plasmid is inactivated. This suggests that hyperexpression of bvgA down-regulates the bvg system.

Electronic Resource

1365-2958

Blackwell Publishing Journal Backfiles 1879-2005

Biology

Medicine

The genes encoding urease were cloned from Bordetella bronchiseptica and the 5.2 kb of DNA essential for expression analysed in a T7 RNA polymerase transcription-translation system. At least four poly-peptides with predicted molecular weights of 69 000, 26 000, 12 200 and 11 000 were found. Partial DNA sequence of the gene encoding the 69 000 Da polypeptide revealed high amino acid identity to the α-subunit of Proteus mirabilis urease, UreC and jack bean urease. A stable, unmarked deletion was constructed in this gene to create a urease-negative mutant of B. bronchiseptica. To assess colonization in a guinea-pig model, the urease-negative strain was inoculated with the urease-positive parental strain in a mixed infection. The urease-negative strain out competed the urease-positive strain in the trachea, lungs and caecum. We demonstrate that urease is not essential for B. bronchiseptica colonization of the guinea-pig respiratory and digestive tracts.

Electronic Resource

1365-2958

Blackwell Publishing Journal Backfiles 1879-2005

Biology

Medicine

Tn5 mutants of B. pertussis. Comparison of various functions of the vir loci of B. bronchiseptica and B. pertussis revealed some interesting differences in the coordinate regulation of virulence factors.

Electronic Resource