Search Results - (Author, Cooperation:D. M. Livingston)
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1Latest Papers from Table of Contents or Articles in PressS. Jirawatnotai ; Y. Hu ; W. Michowski ; J. E. Elias ; L. Becks ; F. Bienvenu ; A. Zagozdzon ; T. Goswami ; Y. E. Wang ; A. B. Clark ; T. A. Kunkel ; T. van Harn ; B. Xia ; M. Correll ; J. Quackenbush ; D. M. Livingston ; S. P. Gygi ; P. Sicinski
Nature Publishing Group (NPG)
Published 2011Staff ViewPublication Date: 2011-06-10Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Animals ; Cell Line, Tumor ; Comet Assay ; Cyclin D1/deficiency/*metabolism ; DNA Damage/radiation effects ; *DNA Repair/radiation effects ; HeLa Cells ; Humans ; Mice ; Neoplasms/genetics/*metabolism/pathology ; Protein Binding/radiation effects ; *Protein Interaction Mapping ; Rad51 Recombinase/*metabolism ; Radiation, Ionizing ; Recombination, Genetic/genetics ; Retinoblastoma Protein/deficiencyPublished by: -
2Articles: DFG German National LicensesStaff View
ISSN: 1432-0983Keywords: Key words Yeast ; Mutations ; Suppressors ; RAD52Source: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract We screened for rad52 suppressors against temperature-sensitive (ts), missense, nonsense, and deletion rad52 mutations. Except for the deletion strain all mutants yielded suppressor candidates, indicating that suppressors completely bypassing the need for RAD52 are rare. Characterization of seven, recessive extragenic suppressors from our screen and two previously identified suppressors revealed that nearly all exhibit allele specificity. The allele specificity is positional in that suppressors that suppress a ts mutation in the C-terminal third of the coding region do not suppress three ts mutations in the N-terminal third. Conversly, suppressors against one of the three N-terminal mutations suppress more than one of these mutations but not the C-terminal mutation.Type of Medium: Electronic ResourceURL: -
3Articles: DFG German National LicensesStaff View
ISSN: 1432-0983Keywords: Key words Yeast ; Mutations ; RAD52 ; RAD51Source: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract We have characterized two rad52 mutations that are cold-sensitive for growth on MMS agar. The mutations change residues 61 and 69, respectively, in the 504 amino-acid open reading frame. Neither mutation has a profound effect on mitotic crossing-over or on gene conversion. One has a severe deficiency in sporulation at all temperatures, while the other has a partial deficiency and reduced spore viability. Both mutants are retarded in growth on MMS agar by a high-copy plasmid expressing RAD51.Type of Medium: Electronic ResourceURL: -
4Articles: DFG German National LicensesStaff View
ISSN: 1572-9931Source: Springer Online Journal Archives 1860-2000Topics: BiologyMedicineNotes: Abstract Microinjection of physiologic quantities of macromolecules into tissue culture cells can facilitate the study of the biological effects of such macromolecules. In this communication, we describe a chemical technique which can be used to microinject proteins into monolayers of intact cells. Protein is loaded into erthrocyte ghosts, and the ghosts are then fused to the monolayer with polyethylene glycol 1000. Receipient cells can be injected with an efficiency of greater than 90% and contain an average of 3.8×106 microinjected molecules per cell. This technique circumvents certain problems encountered in virus-induced microinjection.Type of Medium: Electronic ResourceURL: