Search Results - (Author, Cooperation:D. Lim)

2018-05-19

BMJ Publishing

2044-6055

Medicine

Public health, Open access, Global health

2018-05-16

American Chemical Society (ACS)

Chemistry and Pharmacology

2018-03-09

Oxford University Press

0143-3334

1460-2180

Medicine

2013-07-28

American Association for the Advancement of Science (AAAS)

0036-8075

1095-9203

Biology

Chemistry and Pharmacology

Computer Science

Medicine

Natural Sciences in General

Physics

Amino Acid Motifs ; Amino Acids/metabolism ; Animals ; Cell Line ; Culture Media ; Humans ; Mice ; Multiprotein Complexes ; Naphthyridines/pharmacology ; Peptides/chemistry/*metabolism ; Phosphorylation ; Proteins/antagonists & inhibitors/*chemistry/*metabolism ; Sirolimus/*pharmacology ; TOR Serine-Threonine Kinases/antagonists & inhibitors/*chemistry/*metabolism

2013-04-13

American Association for the Advancement of Science (AAAS)

0036-8075

1095-9203

Biology

Chemistry and Pharmacology

Computer Science

Medicine

Natural Sciences in General

Physics

Anaphase ; Cell Cycle Proteins/chemistry/*metabolism ; Deoxyribonucleases/chemistry/*metabolism ; Enzyme Activation ; GTP-Binding Proteins/*metabolism ; *Mitosis ; Phosphoproteins/chemistry/*metabolism ; Phosphorylation ; Protein Conformation ; Protein-Serine-Threonine Kinases/*metabolism ; Saccharomyces cerevisiae/cytology/*metabolism ; Saccharomyces cerevisiae Proteins/chemistry/*metabolism ; Signal Transduction ; tRNA Methyltransferases/chemistry/*metabolism

2011-06-11

American Association for the Advancement of Science (AAAS)

0036-8075

1095-9203

Biology

Chemistry and Pharmacology

Computer Science

Medicine

Natural Sciences in General

Physics

Animals ; Cell Line ; GRB10 Adaptor Protein/*metabolism ; Humans ; Insulin/metabolism ; Intercellular Signaling Peptides and Proteins/*metabolism ; Mass Spectrometry ; Mice ; Multiprotein Complexes ; Naphthyridines/pharmacology ; Phosphoproteins/metabolism ; Phosphorylation ; Proteins/*metabolism ; Proteome/metabolism ; *Signal Transduction ; Sirolimus/pharmacology ; TOR Serine-Threonine Kinases/*metabolism

2018-03-06

Cold Spring Harbor Laboratory Press

1549-5469

Biology

Medicine

2018-01-25

BMJ Publishing Group

0022-2593

1468-6244

Medicine

Genetic screening / counselling

1540-8183

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Electronic Resource

1474-8673

Blackwell Publishing Journal Backfiles 1879-2005

Chemistry and Pharmacology

Medicine

1 The present study was designed to investigate the secretion of catecholamines (CA) evoked by stimulation of cholinergic receptors and membrane depolarization from the isolated perfused adrenal gland of spontaneously hypertensive rats (SHR) and normotensive Wistar–Kyoto rats (WKYR) at adult age. 2 The wet weight of adrenal gland in SHR was greater than that in WKYR. The CA releasing responses evoked by acetylcholine (5.32 × 10−3 m), and high potassium (5.6 × 10−2 m), a membrane depolarizer, were significantly lower in WKYR than in SHR. 3 The secretory responses of CA evoked by DMPP (10−4 m for 2 min), a selective agonist of neuronal nicotinic receptors, and McN-A-343 (10−4 m for 2 min), a selective agonist of neuronal muscarinic receptors, were also significantly lower in WKYR than in SHR. 4 The CA release evoked by Bay-K-8644 (10−5 m), a dihydropyridine-sensitive Ca2+ channel activator, and cyclopiazonic acid (10−5 m), a selective inhibitor of Ca2+-ATPase in the endoplasmic reticulum, were also significantly greater in SHR than WKYR. 5 Taken together, these experimental results demonstrate that the CA secretion evoked by stimulation of cholinergic (nicotinic and muscarinic) receptors as well as membrane depolarization is enhanced more greatly in the perfused adrenal glands of SHR than in those of WKYR. It is suggested that the augmented CA release in SHR compared with WKYR was involved in essential hypertensive pathogenesis.

Electronic Resource

1471-4159

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Abstract: The effects of acute and chronic administration of diisopropylfluorophosphate (DFP) to rats on acetylcholinesterase (AChE) activity (in striatum, medulla, diencephalon, cortex, and medulla) and muscarinic, dopamine (DA), and γ-aminobutyric acid (GABA) receptor characteristics (in striatum) were investigated. After a single injection of (acute exposure to) DFP, striatal region was found to have the highest degree of AChE inhibition. After daily DFP injections (chronic treatment), all brain regions had the same degree of AChE inhibition, which remained at a steady level despite the regression of the DFP-induced cholinergic overactivity. Acute administration of DFP increased the number of DA and GABA receptors without affecting the muscarinic receptor characteristics. Whereas chronic administration of DFP for either 4 or 14 days reduced the number of muscarinic sites without affecting their affinity, the DFP treatment caused increase in the number of DA and GABA receptors only after 14 days of treatment; however, the increase was considerably lower than that observed after the acute treatment. The in vitro addition of DFP to striatal membranes did not affect DA, GABA, or muscarinic receptors. The results indicate an involvement of GABAergic and dopaminergic systems in the actions of DFP. It is suggested that the GABAergic and dopaminergic involvement may be a part of a compensatory inhibitory process to counteract the excessive cholinergic activity produced by DFP.

Electronic Resource

1077-3118

AIP Digital Archive

Physics

Data are presented demonstrating that the lateral wet oxidation of Al(Ga)As layer is strongly influenced by its thicknesses and heterointerface structures as well as Al compositions. The oxidation length decreases rapidly with decreasing AlAs thickness in the range of 〈80 nm and oxidation nearly stops at a thickness of ∼11 nm. Also, the oxidation rate of AlxGa1−xAs decreases quickly with decreasing Al composition, providing a high degree of oxidation selectivity. AlGaAs layers on both sides of AlAs layer reduce the lateral oxidation rate which is enhanced by the stress induced by oxidized AlAs. © 1996 American Institute of Physics.

Electronic Resource

1471-4159

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Aspirin has been shown to protect against glutamate neurotoxicity via the nuclear factor κB pathway. Some studies have implicated the atypical protein kinase C (PKC) zeta (ζ) isoform in cell protection, but the mechanism involved remains unclear. We show here that aspirin exerts at least some of its effects through PKCζ, decreasing the NMDA-induced activation, cleavage and nuclear translocation of this molecule. Aspirin (acetylsalicylic acid) directly inhibited the protein kinase activity of PKCζ, whereas salicylic acid did not. This direct effect of aspirin on purified human PKCζ is consistent with PKCζ inhibition preventing the NMDA-induced death of cortical neurones. Caspase-3 inhibition blocked the cleavage and nuclear translocation of PKCζ, whereas caspase-1-inhibition did not. Thus, PKCζ (protein kinase Mζ) regulates nuclear events essential for the initiation of the apoptotic pathway. Aspirin protects cells against NMDA-induced apoptosis by means of a novel mechanism targeting PKCζ, a key molecule in inflammatory responses and neurodegeneration.

Electronic Resource

1077-3118

AIP Digital Archive

Physics

The enhanced diffusion of impurities that is seen following ion implantation is rapidly quenched, hence, the name transient enhanced diffusion (TED). The quenching of TED is associated with the annealing of implant damage, either by the diffusion of point defects to the bulk or to the surface. It is variously assumed that either the surface or the bulk is the predominant annealing site. In this work, we explore these assumptions by observing the reduction of TED in a buried marker, when the surface is etched to bring it closer to implanted damage. The results show a considerable reduction in TED with surface etching, demonstrating that the surface plays a key role in annealing implant damage. Numerical modeling allows extraction of the surface recombination length of interstitials at 800 °C. Only a value of 0.1 μm is consistent with the data. All but a very small fraction of implanted interstitials are recombined at the surface with this value. © 1995 American Institute of Physics.

Electronic Resource

1077-3118

AIP Digital Archive

Physics

The kinetics of disilane adsorption and hydrogen desorption during low-temperature, ultrahigh vacuum chemical vapor deposition on Si(001) is investigated in situ in real time by monitoring the instantaneous hydrogen coverage using optical second-harmonic generation. A simple two-site adsorption model and first-order desorption are used to establish a reactive sticking coefficient and to predict the Si(001) epitaxial growth rate. The reactive sticking coefficient is temperature independent between 740 and 920 K and equal to 0.04±0.01. Predicted growth rates are in agreement with published growth rates obtained from gas-source molecular-beam epitaxy of Si(001). © 1997 American Institute of Physics.

Electronic Resource

1077-3118

AIP Digital Archive

Physics

The reactions of atomic hydrogen with boron-doped Si(100) were studied using temperature programmed desorption (TPD). In addition to adsorbing at surface sites, hydrogen penetrates into boron-doped Si(100) samples and gets trapped by forming subsurface boron–hydrogen complexes. H2-TPD spectra, taken after exposure to atomic hydrogen, showed, in addition to the well known dihydride (680 K) and monohydride (795 K) desorption features, two peaks at 600 and 630 K due to decomposition of subsurface boron–hydrogen complexes. Increasing total hydrogen uptake with increasing dosing temperature (1.7 ML at 300 K, 4.2 ML at 500 K), suggests an activation barrier for subsurface hydrogen uptake. A quantitative correlation between boron concentration and subsurface hydrogen uptake is shown. © 1997 American Institute of Physics.

Electronic Resource

1077-3118

AIP Digital Archive

Physics

We study Se δ-doped GaAs grown by low-pressure metalorganic chemical vapor deposition using hydrogen selenide as a doping precursor. The results of capacitance–voltage measurements show that the very sharp doping profile can be obtained by Se δ-doping on an Al-rich surface and Se segregation is also reduced by making an Al-rich surface after δ-doping. It is essential to utilize the δ-doping sequence which does not have a post-δ-doping purge step to minimize the Se evaporation. © 1996 American Institute of Physics.

Electronic Resource

1077-3118

AIP Digital Archive

Physics

The effect of bulk boron incorporation on the second-harmonic generation (SHG) spectrum of Si(001) films grown epitaxially by chemical vapor deposition is studied as a function of doping level and temperature. At room temperature, boron doping (NA∼1018 cm−3) strongly enhances and blueshifts the E1 resonance of the second-harmonic generation spectra to 3.4 eV. Surface hydrogen termination reverses this effect. The observed doping and temperature dependence are modeled as electric-field-induced SHG in the bulk depletion region. The results suggest applications of SHG as an in situ, noninvasive probe of electrically active dopants. © 2000 American Institute of Physics.

Electronic Resource

1365-2222

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Background Hamsters are popular household pets and anaphylaxis after their bites have described. However, the putative allergen has not been identified.Objective This study was conducted to identify the allergen causing dwarf hamster (Phodopus sungoris) bite-induced anaphylaxis.Methods Two children with hamster bite-induced anaphylaxis were enrolled. They both had negative results to skin testing and specific IgE to hamster epithelium. However, they were both allergic to Dermatophagoides pteronyssinus (Der p). Identification of the putative IgE-binding allergens from the hamster saliva was performed using immunoblot analysis.Results A specific IgE-binding component at 21 kD in the hamster saliva was identified. ELISA inhibition tests showed partial inhibition with Der p.Conclusions The putative allergen from the hamster saliva causing dwarf hamster-induced anaphylaxis was identified. Possible cross-reactivity with Der p was demonstrated. Further studies will be needed to identify the exact nature and function of this allergen.

Electronic Resource

1365-2958

Blackwell Publishing Journal Backfiles 1879-2005

Biology

Medicine

Reverse transcriptase, discovered in 1970 in retroviruses, has until recently been found only in eukaryotic organisms. Recently it was shown to occur in two groups of bacteria: myxobacteria and Escherichia coli. The gene for reverse transcriptase is part of a chromosomal genetic element that codes for the production of a branched DNA-RNA compound. In this compound a single-stranded DNA is connected to RNA at a specific G residue by a 2′-5’phosphodiester linkage. The precursor for the DNA-RNA compound is a folded messenger RNA, in which the specific G residue is the initiation point for reverse transcription. In the final DNA-RNA compound, the portion of the RNA transcribed by reverse transcriptase is eliminated by RNase H. The DNA-RNA compound is present in several hundred copies per cell. Its biological function is unknown at present.

Electronic Resource