Search Results - (Author, Cooperation:D. G. Myszka)

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  1. 1
    Staff View
    Publication Date:
    2012-12-14
    Publisher:
    Nature Publishing Group (NPG)
    Print ISSN:
    0028-0836
    Electronic ISSN:
    1476-4687
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Animals ; Bacterial Toxins/*metabolism ; CCR5 Receptor Antagonists ; Cell Death ; Cells, Cultured ; Dendritic Cells/cytology/immunology/metabolism ; Exotoxins/*metabolism ; Female ; Humans ; Immune Evasion ; Immunologic Memory ; Jurkat Cells ; Mice ; Myeloid Cells/cytology/immunology/metabolism ; Receptors, CCR5/*metabolism ; Staphylococcus aureus/immunology/*pathogenicity ; T-Lymphocytes/cytology/immunology/metabolism
    Published by:
    http://www.nature.com/

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  2. 2
    Staff View
    ISSN:
    1572-8943
    Keywords:
    affinity chromatography ; biopharmaceutics ; conformational epitope ; differential scanning calorimetry ; ELISA ; gp120 ; multidomain protein ; OKT4a ; protein stability ; sCD4
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Chemistry and Pharmacology
    Notes:
    Abstract Differential scanning calorimetry (DSC) was used, in conjunction with two functional assays that monitor binding, in a storage stability study on a protein of pharmaceutical interest, the soluble form of the T-lymphocyte multidomain surface receptor, sCD4. DSC monitored structural changes in binding and non-binding domains. ELISA, using the monoclonal antibody OKT4a, and frontal elution affinity chromatography, using the HIV surface glycoprotein, gp120, monitored function of the binding domain. The stability of sCD4 in a solution formulation was followed for up to 30 days at five differentpHs ranging from 5.0 to 7.9 and five different temperatures ranging from −70
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/BF01992848

    Articles: DFG German National Licenses