Search Results - (Author, Cooperation:D. A. Gordenin)
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1Latest Papers from Table of Contents or Articles in PressM. S. Lawrence ; P. Stojanov ; P. Polak ; G. V. Kryukov ; K. Cibulskis ; A. Sivachenko ; S. L. Carter ; C. Stewart ; C. H. Mermel ; S. A. Roberts ; A. Kiezun ; P. S. Hammerman ; A. McKenna ; Y. Drier ; L. Zou ; A. H. Ramos ; T. J. Pugh ; N. Stransky ; E. Helman ; J. Kim ; C. Sougnez ; L. Ambrogio ; E. Nickerson ; E. Shefler ; M. L. Cortes ; D. Auclair ; G. Saksena ; D. Voet ; M. Noble ; D. DiCara ; P. Lin ; L. Lichtenstein ; D. I. Heiman ; T. Fennell ; M. Imielinski ; B. Hernandez ; E. Hodis ; S. Baca ; A. M. Dulak ; J. Lohr ; D. A. Landau ; C. J. Wu ; J. Melendez-Zajgla ; A. Hidalgo-Miranda ; A. Koren ; S. A. McCarroll ; J. Mora ; R. S. Lee ; B. Crompton ; R. Onofrio ; M. Parkin ; W. Winckler ; K. Ardlie ; S. B. Gabriel ; C. W. Roberts ; J. A. Biegel ; K. Stegmaier ; A. J. Bass ; L. A. Garraway ; M. Meyerson ; T. R. Golub ; D. A. Gordenin ; S. Sunyaev ; E. S. Lander ; G. Getz
Nature Publishing Group (NPG)
Published 2013Staff ViewPublication Date: 2013-06-19Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Artifacts ; DNA Replication Timing ; Exome/genetics ; False Positive Reactions ; Gene Expression ; *Genetic Heterogeneity ; Genome, Human/genetics ; Humans ; Lung Neoplasms/genetics ; Mutation/*genetics ; Mutation Rate ; Neoplasms/classification/*genetics/pathology ; Neoplasms, Squamous Cell/genetics ; Oncogenes/*genetics ; Reproducibility of Results ; Sample SizePublished by: -
2Articles: DFG German National LicensesChernoff, Y. O. ; Kidgotko, O. V. ; Demberelijn, O. ; Luchnikova, I. L. ; Soldatov, S. P. ; Glazer, V. M. ; Gordenin, D. A.
Springer
Published 1984Staff ViewISSN: 1432-0983Keywords: Mitotic recombination ; Conversion of deletions ; Intragenic crossingoverSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Summary We have made a large scale analysis of prototrophic products of spontaneous and induced mitotic recombination within LYS2 gene of the yeast Saccharomyces. The mutant alleles staying in heterozygote with the wild type allele were uncovered and analysed. Among thirteen lys2 mutations used in the study three had reduced frequencies of mitotic gene conversion. These rarely converting mutations gave a remarkably high proportion of reciprocal events (up to 38%) in pairwise combinations, never seen for any other pair of alleles studied. Two of these mutations are the deletions of large parts of LYS2 gene. The results suggest that mispairing in the region of deletion blocks the hybrid DNA migration and leads to the reduced conversion ability of deletions. Comparison of uncovered alleles ratio in all allele combinations tested lead us to another hypothesis about bidirectional migration of hybrid DNA.Type of Medium: Electronic ResourceURL: -
3Articles: DFG German National LicensesKarpova, T. S. ; Gordenin, D. A. ; Kozina, T. N. ; Andrianova, V. M. ; Larionov, V. L. ; Inge-Vechtomov, S. G.
Springer
Published 1984Staff ViewISSN: 1432-0983Keywords: Spheroplast and lithium chloride transformation ; Mitotic homozygotization ; Polyploid transformantsSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Summary Spheroplast fusion induced by polyethylene glycol often occurs during transformation of yeast spheroplasts. It leads to the appearance of polyploid transformants. We have developed a method of discriminating between haploid and polyploid transformants, based on qualitative comparison of the frequency of mitotic homozygotization for a recessive marker, lys2, in diploid and polyploid hybrids of transformants crossed with a tester haploid bearing lys2. We have used this method to compare the frequency of appearance of polyploids during transformations of spheroplasts and of intact cells using lithium chloride in the strain DC5. Polyploids constitute from 15% to 74% of transformants after transformation of spheroplasts. In lithium chloride transformation polyploids arose in only one of four experiments and constituted 9% of transformants.Type of Medium: Electronic ResourceURL: -
4Articles: DFG German National LicensesGordenin, D. A. ; Trofimova, M. V. ; Shaburova, O. N. ; Pavlov, Y. I. ; Chernoff, Y. O. ; Chekuolene, Y. V. ; Proscyavichus, Y. Y. ; Sasnauskas, K. V. ; Janulaitis, A. A.
Springer
Published 1988Staff ViewISSN: 1617-4623Keywords: Tn5 ; Transposon excision ; YeastSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Tn5 vector. Nine insertions of Tn5 into the structural part of the yeast LYS2 gene situated in a shuttle epsiomal plasmid were selected. All the plasmids with a Tn5 insertion were used to transform yeast strains carrying a deletion of the entire LYS2 gene or a deletion of the part of LYS2 overlapping the point of insertion. All insertions inactivated the LYS2 gene and were able to revert with low (about 10-8) frequencies to lysine prototrophy. Restriction analysis of revertant plasmids revealed them to be indistinguishable from the original plasmid without Tn5 insertion. DNA sequencing of the regions containing the points of insertions, made for two revertants, proved that Tn5 excision was completely precise.Type of Medium: Electronic ResourceURL: -
5Articles: DFG German National LicensesGordenin, D. A. ; Proscyavichus, Y. Y. ; Malkova, A. L. ; Trofimova, M. V. ; Peterzen, A.
New York, NY [u.a.] : Wiley-Blackwell
Published 1991Staff ViewISSN: 0749-503XKeywords: Transposon Tn5 excision ; recombination ; LYS2 gene ; Life and Medical Sciences ; GeneticsSource: Wiley InterScience Backfile Collection 1832-2000Topics: BiologyNotes: Five complementing recessive mutations that exhibit increased bacterial transposon Tn5 precise excision in yeast Saccharomyces cerevisiae were obtained by ethylmethanesulfonate treatment. One of these mutations (tex1) was submitted to extensive genetic analysis. tex1 is a recessive temperature-sensitive mutation resulting in a 20-100-fold increase in Tn5 excision. It also has increased frequencies of ochre mutation reversion, of forward mutation to canavanine resistance, and loss of chromosome III or its right arm. The possible mechanism of tex1 effects is discussed.Additional Material: 6 Ill.Type of Medium: Electronic ResourceURL: