Search Results - (Author, Cooperation:C. R. Harwood)
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1Latest Papers from Table of Contents or Articles in PressP. Nicolas ; U. Mader ; E. Dervyn ; T. Rochat ; A. Leduc ; N. Pigeonneau ; E. Bidnenko ; E. Marchadier ; M. Hoebeke ; S. Aymerich ; D. Becher ; P. Bisicchia ; E. Botella ; O. Delumeau ; G. Doherty ; E. L. Denham ; M. J. Fogg ; V. Fromion ; A. Goelzer ; A. Hansen ; E. Hartig ; C. R. Harwood ; G. Homuth ; H. Jarmer ; M. Jules ; E. Klipp ; L. Le Chat ; F. Lecointe ; P. Lewis ; W. Liebermeister ; A. March ; R. A. Mars ; P. Nannapaneni ; D. Noone ; S. Pohl ; B. Rinn ; F. Rugheimer ; P. K. Sappa ; F. Samson ; M. Schaffer ; B. Schwikowski ; L. Steil ; J. Stulke ; T. Wiegert ; K. M. Devine ; A. J. Wilkinson ; J. M. van Dijl ; M. Hecker ; U. Volker ; P. Bessieres ; P. Noirot
American Association for the Advancement of Science (AAAS)
Published 2012Staff ViewPublication Date: 2012-03-03Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyComputer ScienceMedicineNatural Sciences in GeneralPhysicsKeywords: Adaptation, Physiological ; Algorithms ; Bacillus subtilis/*genetics/*physiology ; Binding Sites ; Gene Expression Profiling ; *Gene Expression Regulation, Bacterial ; Gene Regulatory Networks ; Oligonucleotide Array Sequence Analysis ; *Promoter Regions, Genetic ; RNA, Antisense/genetics/metabolism ; RNA, Bacterial/genetics/metabolism ; RNA, Messenger/genetics/metabolism ; Regulon ; Sigma Factor/metabolism ; Terminator Regions, Genetic ; *Transcription, Genetic ; *TranscriptomePublished by: -
2Latest Papers from Table of Contents or Articles in PressJ. M. Buescher ; W. Liebermeister ; M. Jules ; M. Uhr ; J. Muntel ; E. Botella ; B. Hessling ; R. J. Kleijn ; L. Le Chat ; F. Lecointe ; U. Mader ; P. Nicolas ; S. Piersma ; F. Rugheimer ; D. Becher ; P. Bessieres ; E. Bidnenko ; E. L. Denham ; E. Dervyn ; K. M. Devine ; G. Doherty ; S. Drulhe ; L. Felicori ; M. J. Fogg ; A. Goelzer ; A. Hansen ; C. R. Harwood ; M. Hecker ; S. Hubner ; C. Hultschig ; H. Jarmer ; E. Klipp ; A. Leduc ; P. Lewis ; F. Molina ; P. Noirot ; S. Peres ; N. Pigeonneau ; S. Pohl ; S. Rasmussen ; B. Rinn ; M. Schaffer ; J. Schnidder ; B. Schwikowski ; J. M. Van Dijl ; P. Veiga ; S. Walsh ; A. J. Wilkinson ; J. Stelling ; S. Aymerich ; U. Sauer
American Association for the Advancement of Science (AAAS)
Published 2012Staff ViewPublication Date: 2012-03-03Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyComputer ScienceMedicineNatural Sciences in GeneralPhysicsKeywords: *Adaptation, Physiological ; Algorithms ; Bacillus subtilis/*genetics/*metabolism ; Bacterial Proteins/metabolism ; Computer Simulation ; Data Interpretation, Statistical ; Gene Expression Regulation, Bacterial ; *Gene Regulatory Networks ; Genome, Bacterial ; Glucose/*metabolism ; Malates/*metabolism ; Metabolic Networks and Pathways/*genetics ; Metabolome ; Metabolomics ; Models, Biological ; Operon ; Promoter Regions, Genetic ; Transcription Factors/metabolism ; Transcription, GeneticPublished by: -
3Articles: DFG German National LicensesLewis, P. J. ; Nwoguh, C. E. ; Barer, M. R. ; Harwood, C. R. ; Errington, J.
Oxford, UK : Blackwell Publishing Ltd
Published 1994Staff ViewISSN: 1365-2958Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: BiologyMedicineNotes: A rapid and sensitive method for detection of cell- and compartment-specific gene expression in individual cells of both Gram-negative and Gram-positive microorganisms is described. The method combines the use of gene fusions to lacZ, and a fluorogenic β-galactosidase substrate, fluorescein-di-(β-d-galactopyranoside), with digitized video microscopy. All of the reporter constructs tested were successfully detected. Secondary staining of the cells with a nucleic acid-specific dye, propidium iodide, allowed cells devoid of nucleic acid to be identified, while cell nucleoid shape and the morphological stage of development could be correlated with the location of β-galactosidase activity. The double-staining procedure was used to show that gene expression can be induced in non-culturable cells of Salmonella enteritidis produced by carbon/nitrogen starvation. The resolution was sufficient to distinguish between cells at different morphological stages of sporulation in Bacillus subtilis. This highly sensitive and rapid method may have many other applications in basic and applied microbiology.Type of Medium: Electronic ResourceURL: -
4Articles: DFG German National LicensesStaff View
ISSN: 1476-4687Source: Nature Archives 1869 - 2009Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsNotes: [Auszug] Liquid cultures of strains of E. coli K-12 producing sex pili due to a derepressed sex factor are commonly granular in appearance5-6. Therefore, when very large clumps of bacteria visible to the naked eye were seen with a particular strain, M878, carrying the derepressed I-like sex factor, \drd\6 ...Type of Medium: Electronic ResourceURL: -
5Articles: DFG German National LicensesStaff View
ISSN: 1432-0614Source: Springer Online Journal Archives 1860-2000Topics: BiologyProcess Engineering, Biotechnology, Nutrition TechnologyNotes: Abstract The secretion of proteins from Bacillus subtilis was studied under physiologically well-defined conditions in continuous cultures at a range of specific growth rates. The kinetics of secretion was analysed by using pulse-chase and immunoprecipitation techniques that allowed both processing and release to be monitored. Growth conditions were selected that were known to lead to significant changes in the anionic polymer composition of the cell wall. Under magnesium limitation only low levels of native proteins were released into the growth medium. In contrast, much higher amounts of released protein were observed under phosphate limitation. Although synthesis of native secretory proteins appeared to be highly regulated, only minor changes in the secretion of heterologous proteins were detected. Comparable kinetics of protein release of cells grown under different conditions indicated similar cell wall permeabilities. The large changes in the amounts of released proteins were not reflected in the production of chaperones and components required for protein secretion. The data suggest that the capacity of the secretion machinery is not a major limiting step in the export of native secretory proteins.Type of Medium: Electronic ResourceURL: -
6Articles: DFG German National LicensesStaff View
ISSN: 1617-4623Source: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Summary In cultures of Escherichia coli K-12 or Salmonella typhimurium LT2 carrying the extra-chromosomal plasmid, colicin factor E2-P9, the incidence of cells forming colicin varied spontaneously between a minimum of ca. 0.01% in the exponential phase of growth and a maximum of ca. 75% in the stationary phase. The number of copies of the plasmid per chromosome remained constant throughout, judging from direct measurement of plasmid DNA and from transduction frequencies. The changes observed therefore reflect changes in the frequency with which each copy is expressed. Individual copies of the colicin factor in a given cell are probably expressed simultaneously, not independently. Colicin producing cells, although killed, do not disintegrate during several hours of incubation.Type of Medium: Electronic ResourceURL: