Search Results - (Author, Cooperation:C. M. Robinson)
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1C. M. Robinson ; J. K. Pfeiffer
American Association for the Advancement of Science (AAAS)
Published 2014Staff ViewPublication Date: 2014-11-08Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyComputer ScienceMedicineNatural Sciences in GeneralPhysicsKeywords: Animals ; B-Lymphocytes/*virology ; Caliciviridae Infections/*immunology ; Enterobacteriaceae/*physiology ; Gastroenteritis/*immunology ; Humans ; Intestines/*microbiology ; Norovirus/*physiology ; *Virus ReplicationPublished by: -
2Staff View
ISSN: 1432-1955Source: Springer Online Journal Archives 1860-2000Topics: BiologyMedicineNotes: Abstract Genetically modified Escherichia coli expressing the Taenia ovis fusion protein GST-45W(B/X) as inclusion bodies were grown in volumes ranging up to 1000 l. Bacteria were inactivated by heat or chemical treatment without affecting immunogenicity. The fusion protein was recovered in a highly immunogenic form from washed inclusion bodies and from urea-solubilised inclusion bodies. The fusion protein was found to be stable in solution after storage at 4°C for up to 2 years. Vaccines formulated with fusion protein from urea-soluble inclusion bodies gave consistently high protection (89–100%) against challenge infection. The methods described enabled the production of sufficient vaccine for large field trials. These trials generated the data required for product registration and manufacture of a vaccine to prevent T. ovis infection in sheep.Type of Medium: Electronic ResourceURL: